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Articles by Jun Chen
Total Records ( 9 ) for Jun Chen
  Wenting Han , Xuan Wang , Jun Qiao , Jun Chen and Shaoping Xue
  To restore the original power, economy and technical indicators of an overhauled diesel engine, the crank lengthening method was adopted and tested in the study. The crank of a Dongfanghong type 4125A diesel engine was extended by 0.75 mm, which is within the range tolerable to the structure. The internal combustion engine rig test method was adopted and a hydraulic dynamometer, comprehensive engine tester, smoke meter and additional instruments were employed to measure the indicators cylinder pressure, speed control characteristics, power, reliability and economy of the diesel engine before and after rebuilding for comparison. The results show that the rebuilding method in which the crank is lengthened increased the maximum power of the diesel engine by 2.32 kW, decreased fuel consumption by 5 g/(kW·h), decreased the exhaust gas temperature by 27°C, decreased the smoke density by 0.2 BSU and increased the mean pressure of various cylinders by 0.31 MPa. The results confirm that the crank lengthening method is effective at restoring the original power, economy and technical indicators of an overhauled diesel engine.
  Junhua He , Zhaozhi Zeng , Jun Chen , Jinquan Wang , Qizhu Chen and Huaben Bo
  Background and Objective: Hypocythemia is frequently developed in hematological and cancer patients. However its current therapies may induce the transmission of both viral and bacterial infection and lead to autoimmune pure red cell aplasia. The Chinese herbs exert their pharmacological effects through a multi-component and multi-target way coupled with its minimal side-effects. Danggui Buxue Tang (DBT) has been widely used for menopausal women as dietary supplements in China. This study aimed to evaluate the quality of Danggui Buxue Tang on the whole and the pharmacological effect from different levels. Materials and Methods: An HPLC method was established to develop chromatographic fingerprints of Danggui Buxue Tang from different areas. The fingerprints were analyzed with similarity evaluation system for chromatographic fingerprint of TCM. Comparison of peripheral blood count in different groups using HS-18 type fully automated hematology analyzer. Semi-solid medium was used to detect the number of erythroid progenitor cells. Erythroid cells numbers were detected by flow cytometry. The one-way analysis of variance (ANOVA) was used to determine statistical significance. Results: The experimental results showed that the fingerprints of the 6 samples of Danggui Buxue Tang had high similarity. So the mutual mode fingerprint for Danggui Buxue Tang was established and used for Danggui Buxue Tang quality control. However, only 17 common peaks in HPLC chromatogram were observed in 6 samples and the relative peak areas of common peaks were obviously different in different samples. Pharmacodynamic experiment showed that Danggui Buxue Tang could increase the number of peripheral reticulocyte, but had no effect on the number of peripheral red blood cells. Danggui Buxue Tang could increase the number of burst-forming unit-erythroid, colony forming unit-erythroid and erythroid cells in bone marrow. Conclusion: The fingerprint technique can be used for the quality evaluation and control of DBT. Danggui Buxue Tang can promote erythropoiesis in acute chemotherapy injured mice.
  Jun Chen , Yachun Wang , Yi Zhang , Dongxiao Sun and Yuan Zhang
  Bio-economic profit models were used on Chinese Holstein production system to evaluate economic values for production traits Milk Yield (MY), Fat Percentage (FP), Protein Percentage (PP)) growth traits Mature Live Weight (LW) and functional traits Age at First Calving (AFC), Calving Interval (CI), Production Lifetime (PL) and Somatic Cell Count (SCC) traits. Economic and production parameters were derived from large scale dairy farms located in Beijing in 2008 to characterize Chinese Holstein production system, which were described according to their growth stage and levels of feed and management environment. Revenue and cost were calculated according to different age group in dairy production. With a fixed number of cows per herd as a basis of evaluation, two milk payment systems were considered. Under payment system based on milk volume, estimated economic values were RMB 1.99 (MY, kg),-4.72 (PP, %), -8.00 (FP,%), 0 (SCC), -4.96 (AFC, days), 1.71 (CI, days), -2.73 (LW, kg) and 1.58 (PL, days), respectively. Under payment system based on milk composition and quality (fat percentage, Protein Percentage, SCC), changes in the economic values were only observed for PP (64.21), FP (41.24), SCC (-393.88), respectively. Sensitivity of economic values was studied, factors considered included the price of milk, price of beef, price of feed and other production variables. Economic values of MY, PP and FP increased significantly with higher price of milk and the beef price had no effect on the economic values of milk production traits. With higher feed price, the economic value for the grow traits and functional traits increased, but that for milk production traits decreased. In summary, evaluated economical value for different traits showed that production and functional traits would have a positive effect, while growth traits would have a negative effect on profitability of Chinese Holstein production system and price of milk and feed were identified as the main factors influencing profit. The bio-economic profit model constructed for Chinese Holstein breeding system could provide general ideas for breeding goals setting in Chinese dairy breeding system in the future.
  Huabin Zhu , Bo Lin , Jun Chen , Haisheng Hao , Xueming Zhao , Shujing Li , Weihua Du , Tong Qin , Yan Liu and Dong Wang
  A rapid and simple PCR sex identification of embryo is very important for bovine embryo transferring. Many sex identification methods using duplex PCR were established according to Sry gene. But the identification process was affected greatly by more primers interaction. In order to decrease the interference from more primers, researchers explored a simple and rapid PCR Method. The sequences of Amelogenin alleles located at both sex chromosomes were downloaded from GenBank. A pair of sex specific primers was designed to span the 63 bp longer insertion sequence in X chromosome. Bovine samples of blood, fibroblasts and demi-embryos were sexed with these primers. Two-temperature PCR cycling program was used in which the extension step was deleted while the denaturizing and annealing steps were shortened to 1 sec. The results shown ideal identification were obtained and observable amplification were also obtained using even single fibroblast. About 20 bovine embryos were identified by this PCR cycling program and 15 embryos (9 females and 6 males) were transferred. The sexing results were confirmed by the anatomically proven sex after parturition, respectively. The comparison of amplification results between blood samples of bovine and human shows the excellent, specificity to bovine. Thus, a simple, rapid and effective PCR sex identification method was established.
  Tracey Baas , Anjeanette Roberts , Thomas H. Teal , Leatrice Vogel , Jun Chen , Terrence M. Tumpey , Michael G. Katze and Kanta Subbarao
 
  Jun Chen , Mingzhu Liu and Shilan Chen
  In this study, a novel semi-interpenetrating polymer network (semi-IPN) hydrogel of kappa-carrageenan-g-poly(methacrylic acid)/poly(N,N-diethylacrylamide) (KC-g-PMAA/PDEA) was prepared using ammonium persulfate as an initiator and N,N,N´,N´-tetramethylethylenediamide as an accelerator at 25 °C. The structure of the hydrogels was characterized by Fourier transform infrared spectroscopy and scanning electron microscope. The effects of the feed components, temperature and pH on the swelling behavior of the hydrogels were studied. The results showed that the introduction of KC-g-PMAA did not change the lower critical solution temperature of the semi-IPN hydrogels, which was the same to the PDEA hydrogel (at 28 °C). With the increase of KC-g-PMAA content, the thermo- and pH-sensitivity of the hydrogel improved, which the swelling rate increased with the increasing content of KC-g-PMAA. The semi-IPN hydrogels showed a fast deswelling and swelling rate. The swelling dynamic transport of the hydrogels was analyzed based on the Fickian equation.
  Jun Chen , Jihong Liu , Tao Wang , Hengjun Xiao , Chunping Yin , Jun Yang , Xiaowen Chen and Zhangqun Ye
  The relaxation mechanisms of tetrandrine (Tet) on the rabbit corpus cavernosum tissue in vitro were investigated. Strips of rabbit corpus cavernosum were mounted in organ chambers. The effects of Tet were examined on isolated muscle strips pre-contracted with phenylephrine (PE) alone, in the presence of NW-nitro-L-arginine (LNNA, a nitric oxide synthase inhibitor), 1-H-[1,2,4]oxadiazolo[4,3-α]quinoxalin-1-one(ODQ, a guanylyl cyclase inhibitor), indomethacin (cyclooxygenase inhibitor), tetraethylammonium (TEA, Ca2+-activated K+ channel blocker), 4-aminopiridine (4-AP, voltage dependent K+ channel blocker) and glibenclamide (ATP sensitive K+channel blocker). The effects of Tet on KCl-induced contraction of isolated muscle strips were also investigated. The procedure of calcium absence-calcium addition was designed to observe the effect of Tet on the two components of the contractile responses to PE based on the source of Ca2+ (extracellular vs. intracellular). Corpus cavernosum strips showed relaxation in response to Tet (10-8 ~ 10-3 mol L-1) in a concentration-dependent manner with an IC50 of 3.73 x 10-5 mol L-1. However, they were not affected by LNNA, ODQ, indomethacin and K+-channel blockers. Tet (10 μmol L-1, 30 μmol L-1) concentration dependently reduced the maximal contraction response of isolated strips induced by KCl to (73.0 ± 3.8) and (41.5 ± 3.4)%, respectively (p < 0.01). In the procedure of calcium absence-calcium addition, Tet 100 μmol L-1 inhibited both intracellular calcium-dependent and extracellular calcium-dependent contraction induced by PE (20 μmol L-1) (p < 0.05). The inhibition ratios were (23.8 ± 7.1) and (40.7 ± 11.2)%, respectively. The results of the present study suggest that Tet possesses a relaxant effect on rabbit corpus cavernosum tissues, which is attributable to the inhibition of extracellular Ca2+ influx and the inhibition of release of intracellular-stored Ca2+, but not mediated by the release of nitric oxide, prostaglandins or by the activation of potassium channels.
  Jinxia Deng , Jun Chen , Ranbo Yu , Guirong Liu , Xianran Xing , Songbai Han , Yuntao Liu , Dongfeng Chen and Linfeng He
  Microwave dielectric powder Ba(Ca1/3Nb2/3)O3 with high B-site cation ordering was synthesized by the molten salt method. Neutron powder diffraction (NPD) and Raman scattering spectra were introduced to investigate the variable ordering degree during the sintering process. It was revealed that the as-synthesized Ba(Ca1/3Nb2/3)O3 powder had a nearly completely ordered structure, and the sintered Ba(Ca1/3Nb2/3)O3 presented a bit higher ordering degree based on the detailed quantitative NPD Rietveld full profile fitting. The complete Raman mode assignment for the Ba(Ca1/3Nb2/3)O3 was presented. The phonon bands could also confirm the order–disorder structural model and increasing ordering degree with the increasing sintering temperature. Final microwave dielectric measurements on sintered samples showed the present compound to tailor other dielectric materials for microwave applications with the dielectric properties of εr = 47.11, and Qf = 1389 GHz, and τf was about 113 ppm/°C.
  Elizabeth R. Sharlow , Karthik V. Giridhar , Courtney R. LaValle , Jun Chen , Stephanie Leimgruber , Rebecca Barrett , Karla Bravo-Altamirano , Peter Wipf , John S. Lazo and Q. Jane Wang
  Protein kinase D (PKD) is a novel family of serine/threonine kinases targeted by the second messenger diacylglycerol. It has been implicated in many important cellular processes and pathological conditions. However, further analysis of PKD in these processes is severely hampered by the lack of a PKD-specific inhibitor that can be readily applied to cells and in animal models. We now report the discovery of the first potent and selective cell-active small molecule inhibitor for PKD, benzoxoloazepinolone (CID755673). This inhibitor was identified from the National Institutes of Health small molecule repository library of 196,173 compounds using a human PKD1 (PKCµ)-based fluorescence polarization high throughput screening assay. CID755673 suppressed half of the PKD1 enzyme activity at 182 nM and exhibited selective PKD1 inhibition when compared with AKT, polo-like kinase 1 (PLK1), CDK activating kinase (CAK), CAMKIIα, and three different PKC isoforms. Moreover, it was not competitive with ATP for enzyme inhibition. In cell-based assays, CID755673 blocked phorbol ester-induced endogenous PKD1 activation in LNCaP cells in a concentration-dependent manner. Functionally, CID755673 inhibited the known biological actions of PKD1 including phorbol ester-induced class IIa histone deacetylase 5 nuclear exclusion, vesicular stomatitis virus glycoprotein transport from the Golgi to the plasma membrane, and the ilimaquinone-induced Golgi fragmentation. Moreover, CID755673 inhibited prostate cancer cell proliferation, cell migration, and invasion. In summary, our findings indicate that CID755673 is a potent and selective PKD1 inhibitor with valuable pharmacological and cell biological potential.
 
 
 
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