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Articles by Jose Rino
Total Records ( 2 ) for Jose Rino
  Jose Rino , Joana M. P. Desterro , Teresa R. Pacheco , Theodorus W. J. Gadella Jr. and Maria Carmo-Fonseca
  Splicing factors SF1 and U2AF associate cooperatively with pre-mRNA and play a crucial role in 3’ splice site recognition during early steps of spliceosome assembly. Formation of the active spliceosome subsequently displaces SF1 in a remodeling process that stabilizes the association of U2 snRNP with pre-mRNA. Fluorescence microscopy shows SF1 and U2AF distributed throughout the nucleoplasm, where transcription occurs, with additional concentration in nuclear speckles, where splicing factors accumulate when not engaged in splicing. Fluorescence recovery after photobleaching analysis in live cells shows that the mobilities of SF1 and the two subunits of U2AF (U2AF65 and U2AF35) are correlated with the abilities of these proteins to interact with each other. Direct binding of SF1 to U2AF65 was demonstrated by fluorescence resonance energy transfer in both the nucleoplasm and nuclear speckles. This interaction persisted after transcription inhibition, suggesting that SF1 associates with U2AF in a splicing-independent manner. We propose that SF1 and U2AF form extraspliceosomal complexes before and after taking part in the assembly of catalytic spliceosomes.
  Jose Rino , Jose Braga , Ricardo Henriques and Maria Carmo-Fonseca
  How we see organisms and cells depends on the tools at our disposal. For over 150 years, biologists were forced to rely on fixed, dehydrated and stained specimens in order to guess how the living cells could function. It all changed abruptly during the last two decades when the rapid development of novel imaging techniques revolutionized the way scientists look at the structures of life alive.
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