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| Articles
by
Jonathan Lalsiamthara |
Total Records (
3 ) for
Jonathan Lalsiamthara |
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G. Patra
,
H. Prasad
,
Jonathan Lalsiamthara
,
J.L. Kataria
,
David Malsawmkima
and
H. Lalrinkima
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A survey was carried out to detect the overall incidence of
histomoniasis in broiler birds in different parts of Mizoram, India. Out of
4000 birds examined, 40 birds were found positive for Histomonas meleagridis
revealing 1% infection rate. Heterakis gallinarum was recovered from
the caeca of positive birds. Gross lesions were found in caeca and liver. Microscopic
examination of stained section of liver tissue revealed numerous circular amoeboid
Histomonas meleagridis together with cellular infiltration. The PCR selectively
amplified 209 BP of the small subunit ribosomal DNA sequence of Histomonas
meleagridis. |
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Gautam Patra
,
M. Ayub Ali
,
Kh. Victoria Chanu
,
Jonathan Lalsiamthara
,
J.L. Kataria
,
Snigdha Hazarika
,
David Malsawmkima
,
R. Ravindran
and
L. Inaotombi Devi
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An outbreak of coccidiosis in laboratory rats has been described in this study. Clinically, out of 50 rats, 30 were died after showing symptoms of anorexia, emaciation and diarrhea. Post mortem examination showed the large and small intestine distended with necrotic and sloughed off mucosal epithelial cells with intestinal content. The examination of the gut contents revealed numerous Eimeria oocysts. After sporulation, the oocysts were identified as those of Eimeria nieschulzi. The infection due to Eimeria nieschulzi is confirmed by Polymerase Chain Reaction (PCR) after amplifying partial (~1.6 kb) nuclear 18S rDNA from DNA of E. nieschulzi. The histopathological examination showed severe damage of the lamina propria of the intestinal mucosa with numerous coccidian developmental stages in the epithelium of small intestine. Biochemical analysis of serum from infested rats presented a significant increase in serum alanine aminotransferase (ALT) and aspartate aminotransferase (AST), glucose, total proteins, globulin and inorganic phosphorous while a decrease in alkaline phosphatase and blood urea nitrogen with insignificant changes in potassium, sodium, magnesium and chloride. |
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G. Patra
,
H. Prasad
,
Jonathan Lalsiamthara
,
J.L. Kataria
,
David Malsawmkima
and
H. Lalrinkima
|
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To obtain data on the incidence of lungworm infestation in piglets in different
parts of Mizoram of India a survey was conducted during the calendar year 2011-2012
based on clinical and postmortem findings and in addition some biochemical and
histopathological changes of affected lungs associated with Metastrongylus
elongates infection were performed. The percent prevalence of lung worm
infection in young piglets (upto 4-6 months old) was 40%. Clinically, infected
piglets were exhibiting chronic and paroxysmal coughing with generalized unthriftiness.
Histologically, tips of the lungs were found to be hard and become grayish or
whitish. Histopathological studies revealed pulmonary emphysema. The alveolar
cellular reaction of the affected lungs showed epithelialization of alveoli
with infiltration of round cells, eosinophils, giant cells, fibers and lungworm
eggs. Biochemical analysis of serum from infected piglets showed a significant
increase in serum Alanine Amino Transferase (ALT), serum glucose, inorganic
chloride, potassium and magnesium with a decrease in serum albumin, total protein
and alkaline phosphatase with insignificant changes in the serum sodium level.
The pork consumption in Mizoram is very high and pigs under four to six months
of age showed the highest mortality rate with respiratory signs in different
parts of this area during the study period. There was a serious herd problem
associated with hypoproteinaemia, hyperglycaemia and reduced growth rate. |
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