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Articles by John Nsor-Atindana
Total Records ( 4 ) for John Nsor-Atindana
  Jauricque Ursulla Kongo-Dia-Moukala , John Nsor-Atindana and Hui Zhang
  Enzymatic hydrolysis of plant protein is used as a source of bioactive peptides. In the present study, defatted corn protein was treated with Flavourzyme to produce hydrolysate with hypocholesterolemic activity for potential application in functional food. Defatted corn protein was prepared by an alkaline method and used as a substrate for enzyme hydrolysis. The derived hydrolysate was assessed for their hypocholesterolemic activity in different in vitro assay systems, including cholesterol micellar solubility inhibition and bile acids binding capacities. The bile acids used were: sodium glycocholate, sodium cholate and sodium deoxycholate. To know much about the defatted corn hydrolysate, the solubility, hydrophobicity and effect of amino acids content of hydrolysate on hypocholesterolemic activity were investigated. Hydrolysis with Flavourzyme for 90 min yielded a DH of 12.25%. The results showed that defatted corn hydrolysate had effective hypocholesterolemic activity. Hydrolysate had 67.42, 19.01, 9.99 and 86.90% of cholesterol micellar solubility inhibition, sodium glycocholate, sodium cholate and sodium deoxycholate, respectively. The amino acid analysis revealed that the predominant hydrophobic amino acids residue were Leucine, Valine, Alanine, Proline, Glycin and Phenylalanine. This result suggested that defatted corn protein hydrolysate could exhibit a hypocholesterolemic activity.
  Daniel Mukunzi , John Nsor-Atindana , Zhang Xiaoming , Arthur Gahungu , Eric Karangwa and Godelieve Mukamurezi
  Moringa oleifera leaf samples from China and Rwanda were used for volatile profile analysis. Volatile compounds were analyzed using the Headspace-Solid Phase Microextraction (HS-SPME)-Gas Chromatography-Mass Spectrometry (GC-MS). A total of 93 volatiles consisting of aldehydes, alcohols, ketones, hydrocarbons, esters, terpenoids and acids were identified. While a total of 61 compounds were contained in the sample from China with acetic acid (12.54% of total volatiles) as the most abundant volatile compound, Rwandan sample contained 59 compounds having hexanoic acid (19.81% of total volatiles) as the most abundant one. Chinese sample showed a higher amount of fat (3.56%) and protein (29.54%) than Rwandan sample fat (3.48%) and protein (25.26%). The ash content was found to be higher in Rwandan sample than in Chinese sample 17.26% and 13.44% respectively. The total polyphenol and total flavonoid contents of Chinese and Rwandan samples were respectively as follow Chinese sample (24.65 mg GAE/g dry weight basis and 39.08 mg Rutin Eq/g dry weight basis) and Rwandan sample (30.01 mg GAE/g dry weight basis; 52.78 mg Rutin Eq/g dry weight basis).
  John Nsor-Atindana , Fang Zhong , Kebitsamang Joseph Mothibe , Mohamed Lamine Bangoura and Camel Lagnika
  This study evaluated the total Phenolic Compound Content (PC) and biological activity of Cocoa Bean Shell (CBS). Proximate compositions of the dry matter (CBS) were investigated, while the antimicrobial activity was of CBS phenolic extracts in 80% acetone, ethanol, methanol and water performed by paper disk diffusion and micro broth dilution methods against 4 bacterial strains. The results showed that CBS consisted of mainly dietary fiber (60%), followed by protein (16.93%), fat (6.87%), polyphenols (4.85%) and moisture (3.73%). The extracts inhibition zones diameter against the tested strains ranged from 16.1 to 9.19 (mm) and were all significantly higher (p<0.05) than the negative control. The minimum inhibitory concentration ranged from 0.78 TPmg/mL to 2.58 TPmg /mL. The relatively high levels of dietary fiber coupled with associated phenolics implies that this by-product might be of interest to the food industry, considering its potential application as a functional ingredient in confectionery, bakery or in the preparation of low-fat, high-fibre dietetic products.
  Mohamed Lamine Bangoura , John Nsor-Atindana , Zhou Hui Ming , Peng Wei , Kebitsamang Joseph Mothibe and Zhu Ke Xue
  The study evaluated physicochemical and content of Resistant Starch (RS) of starches isolated from defatted white foxtail millet and yellow foxtail millet by alkaline extraction. The characteristic of the isolated starches were compared with those of commercial corn starch (CCS). The extraction yielded 42.10% and 39.29% (dry basis) of starch respectively, for white and yellow foxtail millets. The amylose content was highest (35.80mg g-1) in the Yellow Foxtail Starch (YFS) followed by White Foxtail Starch (WFS) which recorded 34.92mg g-1 and CCS (33.98mg g-1) with significant difference (p<0.05). The amylopectin was found in trace amounts for both WFS and YFS, whereas highest value of carbohydrate was found in CCS (98.59 mg g-1) followed by WFS (98.03mg g-1) and YFS (82.79mg g-1). Microscopic examination showed that all starch granules had sizes ranging from 20 to 50 μm with variable irregular shapes. Millet starch indicated highest degree of syneresis and gel consistency. Swelling and solubility increased as temperature increased from 60 to 95°C. Pasting viscosities and maximum peaks were CCS (75.34°C, 3307cP), WFS (76.09°C, 3321cP) and YFS (76.10°C, 3322cP). The RS in raw defatted and hot boiled starches were (15.77-9.17%) in CCS, (13.35-7.46%) WFS and (14.56-8.24%) YFS, respectively and showed a significant difference (p<0.05) among samples.
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