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Articles by Jing Wang
Total Records ( 16 ) for Jing Wang
  Jing Wang , Li-Jun Shi , Peng Wang , Zhan-Zhong Zhao , Miao-Miao Gong , Gang Li , Wei-Feng Yuan and Hong-Fei Zhu
  In order to investigate the new strains circulating in Beijing, 24 positive samples of CPVs were isolated from Beijing of China between 2009 and 2011 and the sequences of the main capsid protein VP2 were obtained. These sequences were analyzed in comparison with the sequences of 29 other strains of CPV from GenBank. Two types of CPV, including CPV-2 and CPV-2a were detected and CPV-2a (with Ala297 mutation) was predominant in Beijing and 4 strains CPV-2a types were found to carry the amino acid substitution Ala440. The unique Ile324 mutation in the VP2 of Beijing CPV isolate was detected as compared with a Tyr324 in the VP2 of the reference CPV strains. A phylogenetic tree was constructed from the VP2 genes. These results showed that most of the isolated Beijing strains classified in a cluster Chinese field isolates which were distinct from American and Italian isolates.
  Yongzhen Huang , Enping Zhang , Jing Wang , Yongtao Huai , Xianyong Lan , Liang Ma , Zhuanjian Li , Gang Ren , Fuying Chen , Chuzhao Lei , Juqiang Wang and Hong Chen
  It is known that the SREBP1c gene is an important gene responsible for adipogenesis and regulation of the expression of genes controlling fatty acid biosynthesis. Its expression levels increase in parallel with obesity. Therefore, the present study focused on screening the genetic variation within bovine SREBP1c gene and analyzing its effect on growth traits in 1035 individuals belonging to four Chinese cattle breeds (QC, NY, JX, CH) using PCR-SSCP, DNA sequencing, and forced PCR-RFLP methods. The results revealed two novel mutations: NC_007317: g. 10781 C > A (457aa), 10914 G > A (502aa). Association analysis with growth traits in the Nangyang breed indicated that: The SNPs in the bovine SREBP1c gene had significant effects on body weight and average daily gain at birth, 6 and 12 months old (P < 0.05 or P < 0.01). Therefore, these results suggest that the SREBP1c gene is a strong candidate gene that affects growth traits in cattle.
  Jiapeng Lin , Liqin Wang , Yuncheng Zhao , Min Hou , Jing Wang and Juncheng Huang
  Oocyte quality directly affects production efficiency of modern assisted reproductive technologies such as the in vitro maturation, fertilization, cultivation of cattle oocytes and sperm injection, embryo transfer and animal cloning. Thus, maternal genes (GDF9, Zar1, Mater and DNMT1) are selected to analyze the relationship between mRNA expressions of these genes and quality of ovine oocyte. This study collects ovine oocytes at GV stage and 12-30 h of in vitro maturation (IVM) respectively, as well as ovine oocytes with the first polar body emitted and not emitted. With the application of quantitative RT-PCR technique, mRNA expression levels of GDF9, Zar1, Mater and DNMT1 of oocytes in the above test groups are tested. It is found that: mRNA relative expression levels of GDF9, Zar1 and Mater at GV stage are the highest (p<0.05), while expression of DNMT1 reaches the highest level at 12 h (p<0.05). With the maturity of oocytes, all gene expression levels gradually decline significantly between 18 and 24 h (p<0.05), while the expression levels of four genes present an upward trend at 30 h (p<0.05). Expression of 4 genes in non-polar body oocytes are all higher than those in polar body oocytes (p<0.05). In the natural bare oocytes, expression of each gene is lower than cumulus-oocytes complex. Thus, the test indicates that GDF9, Zar1, Mater and DNMT1 can be the molecular indicators to determine the quality of ovine oocytes.
  Jing Wang
  This study draws from established bases of research in marketing and Information Systems (IS) to develop an integrated model of user loyalty antecedents to Social Networking (SN) sites. The findings suggest that two general (satisfaction and switching costs) and two context specific (trusting beliefs and habit) variables account for substantial proportion of the variance in user loyalty. Based on the findings, strategies to help SN site develop user loyalty are proposed.
  Chunhu Zhao , Lingyun Dai , Jing Wang , Yu Jian , Zujun Mei , Xufeng Pei , Xiaoqing Xiong , Wensheng Yuan and Fengxue Wu
  Background and Objective: Hypertrophic scar (HTS) is a fibroproliferative disorder which develops from thermal or traumatic injury. The present experiment was intended to evaluate the beneficial effect (anti-scarring) of alpha-lipoic acid (ALA) against post burn hyperscarring rabbit ear model. Materials and Methods: Rabbits were separated into four groups as negative control- HTS group rabbits were induced with full thickness burn wound using a 7 mm red hot plate (100°C). While, the HTS induced rabbits were treated topically with 5, 10 and 20 % ALA, respectively from the 8th day until 28 days. Data were analyzed using one-way ANOVA (analysis of variance) with a significance level of 5%. Results: The contents of collagen (type I and III), as well as glycosaminoglycans (hydroxyproline and hexosamine),were significantly decreased upon treatment with a different dose of ALA. All the irregular/abnormal histological changes were reverted to normal (regular collagen fibres and lesser fibroblast) on addition with ALA in dose concentration fashion. Topical application of ALA for 21 days considerably lowered the scar elevation index (SEI). Moreover, treatment with ALA could markedly up regulate or down-regulate the protein expressions of matrix metalloproteinase-1(MMP-1) and transforming growth factor beta-1 (TGF-β1), respectively. Conclusion: The topical application of ALA could drastically lower the scarring area after a thermal wound in a dosedependent manner. Hence, ALA can be recommended for treating various injury scar especially post-burn hypertrophic scar with standard anti-scarring agents.
  Jing Wang , Liping Zhang , Jianping Wu , Jianlei Jia and Qiang Ding
  Association between lambing performance (single lamb and twins) and reproductive hormones has been explored. Twenty four ewes divided into 4 groups of each 6 individuals were sampled according to the number of lambs in two breeds (Mongolian ewes and Poll Dorset ewes) blood samples from each individual ewe were collected at the three periods: estrus (day 0) estrus (day 11) and post-partum anoestrus. All the seven hormones concentration of the Poll Dorset ewes was higher than that of the Mongolian ewes in the three periods. And the hormones secretion shown different patterns in two breeds. Five hormones concentrations of ewes with twins were higher than that of ewes with single lamb, the reverse situation found in MT and T concentration even a few of them were statistically significant (p<0.05) at the three periods. Finally, the highly positive and negative significant correlations between hormones were detected, this relationship also shown the differences in two breeds.
  Xilong Kang , Xiaolin Sun , Qiuchun Li , Jing Wang , Zhiming Pan and Xinan Jiao
  Toll-Like Receptor 5 (TLR5), a member of the TLR family, specifically recognizes flagellin which contributes to the motility of bacterial pathogens. Therefore, TLR5 plays a crucial role in host defense against flagellated bacteria. In the current study, researchers cloned the TLR5 gene from Jiangquhai pigs (pTLR5). The pTLR5 open reading frame was fused with a FLAG tag by cloning amplified pTLR5 into the pcDNA3.1-FLAG vector. Flp-In-293 cells expressing pTLR5-FLAG (designated pTLR5-Flp-In-293) were obtained using the Flp recombinase system. β-galactosidase activity and zeocin resistance were lost in the pTLR5-Flp-In-293 cells but hygromycin B resistance was acquired. Reverse Transcription-Polymerase Chain Reaction (RT-PCR) and western blot analysis confirmed that pTLR5-FLAG was expressed in pTLR5-Flp-In-293 cells at both the transcriptional and protein levels. Indirect immunofluorescence assay showed that the pTLR5-FLAG protein was located in the cell membrane of pTLR5-Flp-In-293 cells. Stability analysis by fluorescence-activated cell sorting showed that compared with Flp-In-293 cells, the 1st, 10th and 20th generations of the recombinant cell line expressed consistent levels of pTLR5-FLAG protein. Recombinant pTLR5-Flp-In-293 cells stimulated with flagellin from S. typhimurium expressed high levels of IL-8 which indicated that pTLR5 protein expressed in the pTLR5-Flp-In-293 cell line was a functional TLR5 homolog.
  Xinting Zheng , Kemei Peng , Juan Tang , Wei Wang , Ke Xiao , Daiyun Zhu , Shun Lu , Keli Yang , Jing Wang , Pengpeng Sun and Min Chen
  The aim of this study was to determine the effects of supplemental drinking boron on morphology of ostrich chicks’ cerebrum. Twenty four hatched ostrich chicks were divided into six group (I-VI) and supplemented by the water with 0, 40, 80, 160, 320 and 640 mg L-1 boron, respectively for 45 days. Cerebrums were obtained and weighed after dissection then measured the transverse diameter, longitudinal diameter and height of cerebral hemisphere immediately. Paraffin embedded sections of cerebral tissues (4 μm thick) were stained with HE, Nissl’s and argentaffin and then micro photographed. It showed significant increase (p<0.05 or p<0.01) in each anatomy index of group IV in comparison with the other groups while group VI showed significant decrease. Histology study showed that neurons of the cerebrum of group I and II were alike, nerve fibers passed horizontally within the cortex. Neurons of group III were varied in size and shape and with abundance of nerve fibers passed horizontally within the cortex tighter. Whereas neurons of group IV had more types than the rest of groups and were well arranged from the edge to inside by size, the nerve fibers were rich and interweaved. In contrast, neurons of group V and VI were monomorphic with less neurite and nerve fibers were tenuous and sparse. Findings showed that 80-160 mg L-1 supplemental drinking boron promotes cerebrum development, neurons differentiation, neurite formation and nerve fibers elongation of the cerebrum of 0-45 days old ostrich chicks.
  Aihua Yin and Jing Wang
  The single machine total weighted tardiness scheduling problem has been discussed for many years and several effective constructive algorithms have been presented in literatures. The methods for solving this problem are applied among manufacture and logistics fields. This study proposes a quite new constructive algorithm, the Mixed Dispatch Rule (MDR) for solving the problem effectively and efficiently. What the mixed dispatch rule differs from the other dispatch rules is that it takes advantage of not only the jobs’ characters values, such as, process time, due date and the weight but also the values of the objective function for different choices of some job. In fact, in according with the process order of the jobs, at any moment, the status of the unprocessed jobs may be different, i.e., some of them are delayed but others aren’t. So, the characters of these two sorts of jobs are quite different and combining those characters with the objective function’s value can obtain effective dispatch rule. The computing experiment is based on those instances in the classic OR-Library and the computational results show that the algorithm, MDR, is effective and efficient.
  Jing Wang and Mitchell A. Lazar
  The nuclear receptor Rev-erbα is a potent transcriptional repressor that regulates circadian rhythm and metabolism. Here we demonstrate a dissociation between Rev-erbα mRNA and protein levels that profoundly influences adipocyte differentiation. During adipogenesis, Rev-erbα gene expression initially declines and subsequently increases. Remarkably, Rev-erbα protein levels are nearly the opposite, increasing early in adipogenesis and then markedly decreasing in adipocytes. The Rev-erbα protein is necessary for the early mitotic events that are required for adipogenesis. The subsequent reduction in Rev-erbα protein, due to increased degradation via the 26S proteasome, is also required for adipocyte differentiation because Rev-erbα represses the expression of PPARγ2, the master transcriptional regulator of adipogenesis. Thus, opposite to what might be predicted from Rev-erbα gene expression, Rev-erbα protein levels must rise and then fall for adipocyte differentiation to occur.
  Jian Li , Jing Wang , Weiwei Jiang , Xiaowei Dong , Li Pei and Shuisheng Jian
  A stable and low costless tunable erbium doped fiber ring laser using fiber Bragg grating-assisted add-drop filter is proposed and demonstrated. A stable laser output is obtained with a 4 nm tuning range. The power fluctuation, full-width at half maximum and SMSR are measured to be less than 0.50 dB, smaller than 0.015 nm and better than 55 dB in this tuning range.
  Yu-Fu CHEN , Yan-Sui LIU , Jing WANG , Jian-Ping YAN and Xu-Dong GUO
  China has experienced dramatic land use changes over recent decades, with marked environmental and socio-economic consequences. Hengshan County, located in the aeolian-loessial area of Northwest China, was investigated to illustrate land use changes and their implications for environmental and long-term rural economic development. The farmland in Hengshan County significantly decreased during 1990–2003, whereas forest land and grassland increased. The conversion rates of farmland, orchard land, forest land and construction land varied markedly among different periods: 1990–1995, 1995–2000 and 2000–2003. Conversion of orchard land, grassland and construction land was dominant in 1990–1995, whereas the conversion of farmland to forest land mainly occurred in 2000–2003. The results suggested a profound transition in institutional policy and political economy of land management, including implementation of integrated soil erosion control projects, adoption of a market-oriented economy and the ‘Grain-for-Green’ policy, during this period in China. To achieve long-term sustainable land use in Hengshan County, efforts should aim at increasing off-farm income of rural families as well as establishing land-economizing mechanisms to promote land productivity, in addition to conservation measures.
  Jing Wang , Mei-Ying Su , Jin-Qing Qi and Ling-Qian Chang
  Nanometer zirconia (ZrO2) with grain size 20 nm was used to make thick film humidity sensor on silicon substrate. The impedance of the sensor changed from 106 Ω to 102 Ω when the relative humidity (RH) varied from 11% to 98%. The curve of impedance vs frequency showed good linearity when the measured frequency was in between 100 Hz and 1 kHz. The temperature influenced the impedance of the sensor. Complex impedance (Nyquist) diagrams of the sensor at different relative humidities and temperatures were drawn. An equivalent circuit with resistors and capacitors was built to explain the conduction process of the sensor. In low RH range, the conduction process was dominated mainly by conduction and polarization of the grains of nanometer zirconia, while in high RH range, by decomposition and polarization of the absorbed water.
  Jian Liang , Jing Wang , Asim Azfer , Wenjun Song , Gail Tromp , Pappachan E. Kolattukudy and Mingui Fu
  Activated macrophages play an important role in many inflammatory diseases. However, the molecular mechanisms controlling macrophage activation are not completely understood. Here we report that a novel CCCH-zinc finger protein family, MCPIP1, 2, 3, and 4, encoded by four genes, Zc3h12a, Zc3h12b, Zc3h12c, and Zc3h12d, respectively, regulates macrophage activation. Northern blot analysis revealed that the expression of MCPIP1 and MCPIP3 was highly induced in macrophages in response to treatment with lipopolysaccharide (LPS). Although not affecting cell surface marker expression and phagocytotic function, overexpression of MCPIP1 significantly blunted LPS-induced inflammatory cytokine and Formula production as well as their gene expression. Conversely, short interfering RNA-mediated reduction in MCPIP1 augmented LPS-induced inflammatory gene expression. Further studies demonstrated that MCPIP1 did not directly affect the mRNA stability of tumor necrosis factor α and monocyte chemoattractant protein 1 (MCP-1) but strongly inhibited LPS-induced tumor necrosis factor α and inducible nitric-oxide synthase promoter activation. Moreover, we found that forced expression of MCPIP1 significantly inhibited LPS-induced nuclear factor-κB activation. These results identify MCP-induced proteins, a novel CCCH-zinc finger protein family, as negative regulators in macrophage activation and may implicate them in host immunity and inflammatory diseases.
  Shigekazu Tabata , Kimiko Kuroki , Jing Wang , Mizuho Kajikawa , Ikuo Shiratori , Daisuke Kohda , Hisashi Arase and Katsumi Maenaka
  Paired Ig-like type 2 receptors (PILRs) are one of the paired receptor families, which consist of two functionally opposite members, inhibitory (PILRα) and activating (PILRβ) receptors. PILRs are widely expressed in immune cells and recognize the sialylated O-glycosylated ligand CD99, which is expressed on activated T cells, to regulate immune responses. To date, their biophysical properties have not yet been examined. Here we report the affinity, kinetic, and thermodynamic analyses of PILR-CD99 interactions using surface plasmon resonance (SPR) together with site-directed mutagenesis. The SPR analysis clearly demonstrated that inhibitory PILRα can bind to CD99 with low affinity (Kd ~ 2.2 µM), but activating PILRβ binds with ~40 times lower affinity (Kd ~ 85 µM). In addition to our previous mutagenesis study (Wang, J., Shiratori, I., Saito, T., Lanier, L. L., and Arase, H. (2008) J. Immunol. 180, 1686–1693), the SPR analysis showed that PILRα can bind to each Ala mutant of the two CD99 O-glycosylated sites (Thr-45 and Thr-50) with similar binding affinity to wild-type CD99. This indicated that both residues act as independent and equivalent PILRα binding sites, consistent with the highly flexible structure of CD99. On the other hand, it is further confirmed that PILRβ can bind the T50A mutant, but not the T45A mutant, indicating a recognition difference between PILRα and PILRβ. Kinetic studies demonstrated that the PILR-CD99 interactions show fast dissociation rates, typical of cell-cell recognition receptors. Thermodynamic analyses revealed that the PILRα-CD99 interaction is enthalpically driven with a large entropy loss (–TΔS = 8.9 kcal·mol–1), suggesting the reduction of flexibility upon complex formation. This is in contrast to the entropically driven binding of selectins to sugar-modified ligands involved in leukocyte rolling and infiltration, which may reflect their functional differences.
  Shujie Zhao , Sudhakar Ammanamanchi , Michael Brattain , Lin Cao , Amalraj Thangasamy , Jing Wang and James W. Freeman
  Transforming growth factorβ (TGF-β) signals through Smad-dependent and Smad-independent pathways. However, Smad signaling is altered by allelic deletion or intragenic mutation of the Smad4 gene in more than half of pancreatic ductal adenocarcinomas. We show here that loss of Smad4-dependent signaling leads to aberrant expression of RON, a phosphotyrosine kinase receptor, and that signaling by RON cooperates with Smad4-independent TGF-β signaling to promote cell motility and invasion. Restoring Smad4 expression in a pancreatic ductal adenocarcinoma cell line that is deficient in Smad4 repressed RON expression. Conversely, small interference RNA knock down of Smad4 or blocking TGF-β signaling with a TGF-β type I receptor kinase inhibitor in Smad4-intact cell lines induced RON expression. TGF-β-induced motility and invasion were inhibited in cells that express Smad4 and that have low levels of RON compared with isogenically matched cells that were deficient in Smad4. Furthermore, knocking down RON expression in Smad4-deficient cells suppressed TGF-β-mediated motility and invasion. We further determined that Smad4-dependent signaling regulated RON expression at the transcriptional level by real-time reverse transcription PCR and RON promoter luciferase reporter assays. Functional inactivation by site-directed mutations of two Smad binding sites on the RON promoter inhibited TGF-β-mediated repression of RON promoter activity. These studies indicate that loss of Smad4 contributes to aberrant RON expression and that cross-talk of Smad4-independent TGF-β signaling and the RON pathway promotes an invasive phenotype.
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