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Articles by Jing Li
Total Records ( 14 ) for Jing Li
  Xiaobo Zheng , Lijuan Qing , Zhiwan Zheng , Jiao Li , Rong Xiao , Libin Wang , Yinzhu Yao , Boqing Wu , Huihao Xu , Maosen Xu , Jing Li , Qun Wu and Liang Ge
  Leydig cells were isolated from the testis of 3 week old Rongchang piglets. Its activity, purity and function were qualified before being cultured in vitro. Then, they were respectively treated with 0.125, 0.25, 0.5, 1 and 2 μmol L-1 c-jun ASODNs and the expression of c-jun mRNA were detected by FQ-PCR. To study the effect of c-jun on proliferation of elemental status leydig cells in piglets, the leydig cells had been treatedwith c-jun ASODNs and the growth inhibition ratio of leydig cells were detected by MTT method and cell cycle phase distribution were examined by flow cytometer analysis. The FQ-PCR assays results indicated that c-jun mRNA expression decreased with the rise of c-jun ASODNs concentration. Only 0.125 μmol L-1 c-jun ASODNs could reduce the expression of c-jun gene significantly (p<0.01). And the expression no longer went down when the concentration was up to 1 μmol L-1. Through MTT analysis, it is found that c-junASODNs could inhibit the growth of leydig cells significantly (p<0.01). There is a positive correlation between c-jun ASODNs concentration and growth inhibition ratio of leydig cells (r = 0.8966).
  Chunli Chen , Yonglong Lu , Xiang Zhang , Jing Geng , Tieyu Wang , Yajuan Shi , Wenyou Hu and Jing Li
  The current state of concentrations of perfluorooctane sulfonate (PFOS) and perfluorooctanoic acid (PFOA) in China is presented. While products that are known to degrade to either PFOS or PFOA have been used in China, concentrations in environmental media have been reported to be relatively low across China. Greater concentrations of PFOS and PFOA were observed in southern and eastern China than in other areas of China. Concentrations of PFOS and PFOA were relatively great in the Huangpu River, with concentrations of 20.5 ng l-1 and 1590 ng l-1, respectively. Surface waters of Dongguan and Shanghai were more contaminated by PFOS and PFOA than that of other cities. Dongguan was the only city in China in which PFOS value in surface water exceeded the water quality criterion, while PFOA concentration in Shanghai was 152 ng l-1. Similar to other contaminants, point-source pollution was also the common pattern of PFOS and PFOA contamination. Concentrations of PFOS in human blood in China were relatively greater in China than other countries, with drinking water contamination given as the most likely source. Concentrations of PFOS in human blood have increased from the 1980s to the 2000s, while such a trend was not observed for PFOA.
  Jing Li , Yonglong Lu , Wentao Jiao , Tieyu Wang , Wei Luo and John P. Giesy
  Tianjin Bin Hai New Area (BHNA) is the third largest economic zone in China. This is an older industrial area that has been developing rapidly but with many historic sources of contamination. The concentrations of 16 individual polycyclic aromatic hydrocarbons (PAHs) listed for priority control by the US EPA were quantified in 105 surface soil samples by the use of gas chromatography mass spectrometry (GC/MS). Multivariate statistical methods were used to characterise the mixtures and make comparisons in the patterns among areas. Concentrations varied among areas, ranging from moderate to relatively high. The coefficients of determination between concentrations of individual PAHs and total concentration of PAH (∑ PAH) increased with the number of rings. The first three factors from the principal component analysis (PCA) explained 81.6% of the total variation, while ten higher molecular weight (HMW) PAHs` loadings for factor 1 accounted for 51.0%. Based on the patterns of relative loading proportions of individual PAHs calculated by PCA, the primary sources were coal combustion, traffic emissions including gasoline, diesel oil, and the burning of natural gas. The spatial distribution of concentrations of fluorene had a poor spatial autocorrelation in the study area. The sites with the greatest potential risk due to exposure to PAHs were mainly distributed around the chemical industry parks.
  Wei Xiong , Yubing Yu and Jing Li
  The change of mechanical design requirements is throughout the whole life cycle, if not handled timely and effective, will affect the component quality and mechanical product development speed. Firstly, the traditional quality function deployment is extended to Dynamic Quality Function Deployment (DQFD) in order to adapt to the changing requirements, meanwhile the author builds three-dimensional house of quality as its core tool. According to the vagueness and uncertainty of customer requirements, the author provides the systematic analytical framework of mechanical design requirements based on DQFD combining with grey system theory especially GM (1, N) and calculate the importance of requirements and the relationship between requirement and technology by means of grey relational algorithm and then converted to sequence of technical characteristics importance. Through the application in the design and development of mechanical product in the future, it will be proved to reduce the cost of information collection and improve the accuracy of information processing and improve the quality and credibility of mechanical product and component.
  Yunsheng Zhang , Jing Li , Xiaoxiang Hu and Ning Li
  H3K27 methylation plays an important role in the regulation of gene expression and chromatin stability and its expression is dynamically regulated by both specific methylases and demethylases. During development, the regulation of these enzymes is fine-tuned to co-determine the appropriate level of H3K27 methylation thus ensuring correct gene expression. However, it is not completely clear how this regulation is realized. Here, we found that Akt (Akt1 and Akt2), H3K27 methylases (Ezh1 and Ezh2) and demethylases (Jmjd3 and Utx) displayed varying levels of RNA and protein expression in mouse cell lines. Ezh2 was upregulated (p<0.05) at the protein level in an IGF-1-treated C2C12 cell line. The overexpression of p-Akt through myr-Akt vector transfection greatly downregulated the mRNA levels of Ezh1, Jmjd3 and Utx (p<0.01) but not Ezh2 (p>0.05). Therefore, the data suggest that the expression of both H3K27 methylase and demethylase genes is involved in the activation of the IGF-1/PI3K/Akt pathway.
  Jing Li , Lihua Zhao , Jing Gao and Lirong Ran
  This study aims to conduct a preliminary exploration on the application value and prognosis of the insulin therapy in the burn shock resuscitation by observing the impact of insulin on the shock resuscitation fluid requirement, urine output and organ function of patients with major burns during the shock stage. Fifty eight patients with major burns including 31 males and 27 females admitted to the Burn Department of the hospital during the period from February, 2012 to December, 2012 were randomly arranged in an insulin treatment group and a control group, each consisting of 29 patients. The initial resuscitation fluid replacement was calculated based on the Ruijin formula, i.e., the total volumes of the colloid (plasma) and crystal (lactated Ringer’s solution) were calculated on a 1.5 mL/kg/1% TBSA basis and the ratio of crystal to colloid was between 1:1 and 2:1. The shock resuscitation indicators and platelet changes were tested. The unit colloid volume of the treatment group in the first 24 h was 0.98±0.29 mL/kg/1% TBSA which was significantly lower than (1.16±0.32) mL/kg/1% TBSA in the control group. Meanwhile, the unit urine outputs during the shock stage for the treatment group and the control group were 1.90±0.68 and 1.37±0.61 mL/kg/h, respectively. The treatment group showed significantly higher unit urine output than the control group. The differences between the two groups were statistically significant (p<0.05). The TBIL, DBIL, BUN and Cr levels between the patients in the two groups had no significant difference one day after burn. Since day 2 after burn, the treatment group showed lower levels of these indicators than the control group at various time points and the difference had statistical significance (p<0.05) at most time points. Platelet counting of the control group was higher than the treatment group on day 3, 6, 9 and 12. The platelet counting between the two groups showed statistical significance (p<0.05). The APACHE II score of the control group (6.49±2.18) was significantly lower than that of the treatment group (16.25±3.81) in the first 24 h after the admission. The APACHE II scores of the two groups had statistical significance (p<0.05). The application of insulin during the shock stage can reduce the colloid/crystal fluid requirement for resuscitation and increase the urine output of the patient with major burns. This therapy can also extenuate tissue damage and protect organ function. The study shows potential promise of the application of insulin in the shock resuscitation from severe burns.
  Lei Meng , Jing Li , Zhengang Sun , Xuefang Zheng , Hui Chen , Dapeng Dong , Yanyu Zhu , Yan Zhao and Jing Zhang
  Two new divalent metal aminodiphosphonates with a layered structure, Mn2[(HL)(H2O)F]·H2O (1) and Cd3.5[(HL)L] (2) (H4L = CH3CH2CH2N(CH2PO3H2)2), have been hydrothermally synthesized and characterized by single-crystal X-ray diffraction as well as with infrared spectroscopy, elemental analysis and thermogravimetric analysis. In 1, two MnO4F2 and two MnO5F polyhedra are interconnected via edge-sharing into a tetramer, and such tetramers are bridged by the diphosphonate ligands into a Mn(II) phosphonate layer in the ab-plane. The structure of 2 also features a 2D layered structure, in which the CdO5N and CdO6 polyhedra are interconnected into a 1D chain. The chains are then cross-linked via phosphonate oxygen atoms to form Cd(II) phosphonate layers in the ab-plane.
  Zhang Peng Li , Yong Heng Xing , Yuan Hong Zhang , Guang Hua Zhou , Chun Guang Wang , Jing Li , Xiao Qing Zeng , Mao Fa Ge and Shu Yun Niu
  Two new monomeric complexes of oxovanadium(IV), [VO(pzH)(HMPA)2]2 · 4H2O (1) and VO(OH)(dmpzH)2(C6H5COO) (2) (H2MPA = 5-methyl-1H-pyrazole-3-carboxylic acid, pzH = pyrazole, dmpzH = 3,5-dimethyl pyrazole), have been synthesized from reaction of VOSO4 · nH2O with respective ligands. The compounds were characterized by elemental analysis, IR spectra and X-ray diffraction. Structural analysis of 1 and 2 gave the following parameters: 1, monoclinic, P2(1)/n, a = 11.1729(13) Å:, b = 23.965(3) Å:, c = 13.5591(15) Å:, β = 99.969(2)°, V = 3575.8(7) Å:3, Z = 4; 2, orthorhombic, Pbcn, a = 13.197(4) Å:, b = 15.898(5) Å:, c = 18.192(6) Å:, V = 3817(2) Å:3, Z = 8. In each complex the vanadium is a distorted tetragonal bipyramid, which is typical for oxovanadium(IV) complexes. In both complexes, inter- and intra-molecular hydrogen bonds are also discussed. Complex 1 exists as an infinite chain along the b-axis by intermolecular hydrogen bonds. In addition, thermal analysis and quantum chemistry calculations were performed for analyzing the stability of the complexes.
  Jing Li , Jin-Zhou Li , Heng-Qiang Zhang , Yong Zhang and Jing-Qi Li
  A new complex, [Ni(PMFP)2(C2H5OH)2] (HPMFP = 1-phenyl-3-methyl-4-(2-furoyl)-5-pyrazolone), has been synthesized and characterized by elemental analysis, IR, UV, and fluorescence spectra, thermal analysis, and X-ray single crystal diffraction. Its crystal structure is in an orthorhombic system, space group Pbca with cell parameters: a = 15.2269(14) , b = 9.3399(9) , c = 22.794(2) , and Z = 4, S = 1.019. The Ni lies at an inversion center and has a slightly distorted octahedral coordination environment with four oxygens of the pyrazolone rings in the equatorial plane and two ethanols in axial positions. The compound displays O-H···N and weak C(6)-H(6)···O(4) hydrogen bonds. The fluorescent emission is at 539 nm.
  Ying Yan , Hengli Zhang , Yang Liu , Jing Li , Pengfei Sha , Jingliang He , Huaijin Zhang and Jianguo Xin
  A 20.2 W laser-diode-end-pumped Nd:YVO4 slab laser with a hybrid resonator at 1342 nm was demonstrated. The slope efficiency and optical-to-optical conversation efficiency were 30% and 23%, respectively. At output power of 16 W, the M2 factor in stable direction was 2.3, and in the unstable direction was 1.2.
  Qiang Liu , Jing Li , Xiao-Xia Shen , Rui-Guang Xing , Jie Yang , Zhengang Liu and Bo Zhou
  Hantzsch 1,4-dihydropyridine (HEH), a well-known model compound of coenzyme NAD(P)H was found as an efficient reducing agent in hydrogenation of unactivated olefins catalyzed by Pd/C. α,β-Unsaturated ketones also underwent hydrogenation, affording the corresponding saturated ketones selectively.
  Jing Li , L. Andy Chen , Courtney M. Townsend , Jr. and B. Mark Evers
  Neurotensin (NT) is a gut peptide that plays an important role in gastrointestinal secretion, motility, and growth as well as the proliferation of NT receptor-positive cancers. Protein kinase D (PKD) family members (PKD1, 2, and 3) have been identified as important regulators of secretory transport at the trans-Golgi network. Previously, we showed that PKD1 contributes to stimulated NT secretion; however, the mechanisms are not entirely clear. Here, we show that Kidins220, which is a substrate of PKD proteins in neuroendocrine cells, is localized in the ends of the processes of BON cells, similar to the expression pattern of NT vesicles, and translocates to the membrane and large vesicle-like structures formed in response to phorbol 12-myristate 13-acetate treatment. The short hairpin RNA targeting Kidins220 inhibits NT secretion in parental BON cells or BON cells stably expressing the gastrin-releasing peptide receptor treated with either phorbol 12-myristate 13-acetate or bombesin, respectively. Furthermore, we demonstrate that endogenous PKD1, PKD2, and Kidins220 co-exist with NT-containing vesicles. Overexpression of the kinase-dead PKD1 abrogates Kidins220 expression and NT vesicle formation. Our data establish a physiological link between the PKD/Kidins220 pathway and NT-containing vesicles and suggest the role of this pathway in the regulation of hormone secretion. Because NT is an important gut hormone that affects secretion, inflammation, and both normal and tumor cell growth, our findings identify a novel signaling pathway that may be amenable to drug targeting for clinical applications.

  Mohsen Rajabi , Erik de Leeuw , Marzena Pazgier , Jing Li , Jacek Lubkowski and Wuyuan Lu
  Mammalian α-defensins, expressed primarily in leukocytes and epithelia, play important roles in innate and adaptive immune responses to microbial infection. Six invariant cysteine residues forming three indispensable disulfide bonds and one Gly residue required structurally for an atypical β-bulge are totally conserved in the otherwise diverse sequences of all known mammalian α-defensins. In addition, a pair of oppositely charged residues (Arg/Glu), forming a salt bridge across a protruding loop in the molecule, is highly conserved. To investigate the structural and functional roles of the conserved Arg6–Glu14 salt bridge in human α-defensin 5 (HD5), we chemically prepared HD5 and its precursor proHD5 as well as their corresponding salt bridge-destabilizing analogs E14Q-HD5 and E57Q-proHD5. The Glu-to-Gln mutation, whereas significantly reducing the oxidative folding efficiency of HD5, had no effect on the folding of proHD5. Bovine trypsin productively and correctly processed proHD5 in vitro but spontaneously degraded E57Q-proHD5. Significantly, HD5 was resistant to trypsin treatment, whereas E14Q-HD5 was highly susceptible. Further, degradation of E14Q-HD5 by trypsin was initiated by the cleavage of the Arg13–Gln14 peptide bond in the loop region, a catastrophic proteolytic event resulting directly in quick digestion of the whole defensin molecule. The E14Q mutation did not alter the bactericidal activity of HD5 against Staphylococcus aureus but substantially enhanced the killing of Escherichia coli. By contrast, proHD5 and E57Q-proHD5 were largely inactive against both strains at the concentrations tested. Our results confirm that the primary function of the conserved salt bridge in HD5 is to ensure correct processing of proHD5 and subsequent stabilization of mature α-defensin in vivo.
  Jinhua Wang , Chunjing Bian , Jing Li , Fergus J. Couch , Kangjian Wu and Robert Chunhua Zhao
  Expression of the BRCA2 tumor suppressor gene is tightly linked to its roles in DNA damage repair and maintenance of chromosomal stability and genomic integrity. Three transcription factors that activate (USF, NF-κB, and Elf1) and a single factor that represses (SLUG) BRCA2 promoter activity have been reported. In addition, a 67-bp region (–582 to–516) associated with inhibition of promoter activity has been identified. However, it remains unclear how the 67-bp region contributes to regulation of BRCA2 expression. Here, we describe the affinity purification of a 120-kDa protein that binds to a silencer-binding region within the 67-bp repression region of the BRCA2 promoter. Mass spectrometry revealed the identity of the protein as poly-(ADP-ribose) polymerase-1 (Parp-1). Gel shift, antibody super-shift, and chromatin immunoprecipitation (ChIP) assays demonstrated that Parp-1 is associated with the BRCA2 promoter both in vitro and in vivo. Furthermore, Parp-1 inhibitors (either 3-AB or NU1025) and Parp-1 gene specific siRNA resulted in increased levels of endogenous BRCA2 expression. Inhibition of Parp-1 activity (by 3-AB) reduced histone 3 lysine 9 acetylation and blocked Parp-1 binding to the BRCA2 promoter. These results indicate that Parp-1 down-regulates BRCA2 expression through an interaction with a repression region of the BRCA2 promoter.
 
 
 
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