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Articles by Jing Fang
Total Records ( 2 ) for Jing Fang
  Xing Lv , Haibin Zhu , Xiang Gao , Xiaojian Song , Yuanhui Ma , Xiaoying Wang , Jing Fang and Jue Zhang
  The objective of this study is to perform mid-field (0.4T) Magnetic Resonant Imaging (MRI) on a distal forelimb of a healthy Mongolian yearling horse and obtain a detailed cross-sectional comparison between the MRI and anatomy images. T1-weighted, T2-weighted, PD-weighted and STIR sequence were performed to acquire images of the horse distal limb on the transverse plane. After imaging, the limb was frozen and cut into slices to get anatomy photos. Comparisons between the MR images and corresponding frozen cross-sections of the horse limb were made to identify the anatomic details of the horse limb. The results of this study indicate that mid-field MRI can be used to diagnose lesions in the equine distal forelimb at an early stage of lameness. Besides, this study also provides an atlas of gross anatomy of the carpus and fetlock in mid-field MRI which may benefit veterinary clinical evaluation and medical research.
  Zhicai Zuo , Hengmin Cui , Xi Peng , Junliang Deng , Jing Fang , Ya Wang and Zhihua Ren
  The objective of this study was to determine cytokine and chemokine microarray profiles in lung and Hilar Nodes (HN) from pigs infected with Actinobacillus Pleuropneumoniae (APP). Twenty pigs were randomly assigned to one of two groups: Control Group (CG) and inoculated with APP (TG). The infected-APP pigs’ lung exhibited significantly (p<0.05) greater levels of chemokines CCL2, CCL20, IL8 and slightly increase levels of chemokines CCL4, CCL5 and CXCL2 while significantly(p<0.05) decrease levels of chemokines CXCL10 and CXCL12. APP infection significantly (p<0.05 or 0.01) stimulated expression of cytokines IL-18, IL-6, TNF, GM-CSF, CASP3, CASP8 and significantly (p< 0.05 or 0.01) suppressed expression of cytokines CD40, IRF1 in lung. Cytokines in infected-APP pigs’ lung, IL-1A, IL-27, IRF3, IL-10 were slightly increased and CASP1, IRF7, IL-12B, IL-2 were slightly decreased. Relative cytokine and chemokine microarray data in HN indicated that APP infection significantly (p<0.05) stimulated expression of cytokine IL-6 and significantly (p<0.05 or 0.01) suppressed expression of cytokines CXCL12, CD40 and CASP1. In conclusion, 26 cytokine and chemokines mRNA expression levels in lung and HN obtained from infected-APP or control swines were elucidated in this study. This research provided evidence that the increased severity of lesions in the infected-APP swines was associated mainly with alterations of cytokine and chemokines microarray profiles, especialy in lung. The changes of all the cytokines in lung and HN can lead stem cells to produce granulocytes (neutrophils, eosinophils and basophils) and monocytes and also promoted neutrophil and macrophages to phagocytose bacterial and foreign antigen at the site of inflammation. Defense function of pig infection with APP was enhanced while immune function was weakened.
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