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Articles by Jin Woo Jun
Total Records ( 2 ) for Jin Woo Jun
  Jin Woo Jun , Yeon Soo Chung , Sae Kil Yun , Hyoun Joong Kim , Ji Young Chai and Se Chang Park
  Corynebacterium pseudotuberculosis and Corynebacterium ulcerans are recognized as a veterinary pathogen and causing considerable economic impact due to the inefficacy of antimicrobial therapy. Diphtheria toxin can cause myocardial and neurological damage and diphtheria toxin gene detection is considered as a suitable procedure for determining the toxigenicity of corynebacterial isolates. Because there are few studies examining Corynebacterium in duck, researchers evaluated the incidence, toxigenicity and antibiotic susceptibility of Corynebacterium strains from moribund ducks in South Korea. In addition, researchers performed Direct Genome Restriction Enzyme Analysis (DGREA) for the subtyping of Corynebacterium strains. A total of 15 C. pseudotuberculosis strains and 2 C. ulcerans strains were identified using the Vitek System®2, PCR amplification and 16S rRNA gene sequencing. All of the strains used in this study possessed the diphtheria toxin gene. Additionally, the results of antibiotic susceptibility testing showed that 15 of the 17 strains exhibited resistance to multiple antibiotics. In this study, DGREA yielded results that were dependent on the isolation area which could be useful for tracing the source of infection and indicate the potential application of this technique in disease prevention and disinfection.
  Jee Eun Han , Ji Hyung Kim , Jin Woo Jun , Hyoun Joong Kim , Sae Kil Yun , Ji Young Chai and Se Chang Park
  A total of 28 Aeromonas strains obtained from water and diseased fish in South Korea were examined for virulence gene screening. Strains were tested by multiplex PCR for the presence of 2 β-hemolysins genes and 3 Type III Secretion System (TTSS) genes. All A. hydrophila strains carried both aerA genes and ahh1 genes and 5 out of the 8 A. sobria strains carried asa1 genes for β-hemolysins. Additionally, 11 strains carried aopB, aopD or ascV genes for TTSS (aopB gene was detected in 7 A. hydrophila strains and 4 A. sobria strains, aopD gene was detected in 5 A. hydrophila strains and 1 A. sobria strains and ascV gene was detected in 4 A. hydrophila strains and 3 A. sobria strains). Interestingly, some sequence of TTSS genes from tested A. hydrophila showed strong genetic variation to those of the reported A. hydrophila, the aopB genes of strain (SNUFPC-A3) was 94% homologous to A. salmonicida (Accession No. AJ616218.2) those of strains (SNUFPC-A10 and CASAH2) were 98% homologous to A. sobria (Accession No. EF215451.1) and the aopD genes of strain (CASAH2) was 98 homologous to A. sobria (Accession No. EF215451.1).
 
 
 
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