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| Articles
by
Jin Dai |
Total Records (
2 ) for
Jin Dai |
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Jin Shen
,
Yanwei Mao
,
Yimin Zhang
,
Xiao Song
,
Jin Dai
,
Peng Li
,
Rongrong Liang
,
Lixian Zhu
and
Xin Luo
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Differential proteomics in electrical stimulated M. longissimus of Chinese yellow crossbred
bulls (ES: 42 V, 50 Hz, 0.7 A for 40 sec) were carried out using two-dimensional electrophoresis technology.
Twelve protein spots had reduced expression at 1 and 3 days post Electrical Stimulation (ES) of samples which
were divided into nine categories: desmin, troponin T alpha isoform, myosin binding protein H, creatine kinase
(two), triosephosphateisomerase (two), peroxiredoxin-6 (two), phosphatidylethanolamine-binding protein,
histone H3.3-like isoform 2, methyltransferase. Biological function analysis of these proteins indicated ES
affected tenderness via four pathways: glycolytic metabolic pathway, calpains pathway, lysosomal pathway
and oxidative stress pathway. Correspondingly, we concluded that ES could accelerate the decline of pH and
the release of lysosomal enzymes, activate the calpain system and accelerate oxidation and apoptosis. All these
combined effects accelerate the meat tenderization process. Results provide a relatively comprehensive
understanding of the influences that ES exerted on beef tenderness improvement from the perspective of
proteins changing. Furthermore, the results indicate that more attention should be paid on the contribution of
lysosomal to beef tenderness and the effects of oxidative stress on beef tenderness point out the further
research direction and related experiments have been designed to verify such specific mechanism. |
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Wei Chen
,
Ting Huang
,
Jin Dai
,
Wenting Liu
,
Julong Cheng
and
Yunfeng Wu
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Tobacco mosaic virus (TMV) and Potato virus Y (PVY) are two destructive plant viruses which cause significant economic losses to tobacco. Breeding resistant cultivars is one of the most important strategies to control these viruses. In this study, the responses to TMV and PVY of 22 tobacco cultivars were evaluated based on resistance index and virus titre obtained from ELISA in both greenhouses and fields during five years (2007-2011). Nicotiana glutinosa resistant to TMV and Virgin A Mutant resistant to PVY were used as resistant checks; NC89 susceptible to both TMV and PVY was used as the susceptible check. The results showed that there were significant differences in disease index and ELISA value among the 22 tobacco cultivars after inoculation. Specifically, 2 cultivars (Coker86 and Jiyan5) were identified to be resistant or moderate resistant to TMV and 3 cultivars (Liaoyan8, Jinxing6007 and Qinyan98) to PVY, while the other cultivars were susceptible or moderate susceptible to TMV or PVY. The resistant cultivars may be used as resistant resources to TMV and PVY in future breeding projects. |
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