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Articles by Jie Qin
Total Records ( 3 ) for Jie Qin
  Hong-Quan Chen , Han-Qin Wei , Jie Qin and Hua Chen
  To understand prolactin gene variation and its association with egg productivity of goose breeds, the polymorphism of goose prolactin gene and its distribution patterns in different goose breeds were investigated. Six goose breeds selected were Wanxi, Rhine, White Roman, Landaise, Yangzhou and Sichuan. DNA samples of all goose breeds were extracted from goose wing’s blood. The polymorphisms in the 5’-untranslated region of prolactin gene were examined using BstYI enzyme digestion and their distribution patterns were analyzed in different goose breeds. The promoter efficiency of mutational gene was measured using luciferase reporter gene assays. The results showed that a mutation locus, G-10T, located at the bottom nucleotide 10th of the 5’-untranslated region. The G allele frequencies of the six breeds listed above were 0.7857, 0.9778, 0.9493, 0.9054, 0.1479 and 0.2117, respectively. The goose breeds with higher T allele frequencies had higher egg productivity than that with higher G allele frequencies in the practice. The efficiency of promoter with G allele enhanced 26.14% (p<0.01) than that with T allele. The results suggest that the egg productivity of goose breeds may be associated with G-10T locus of prolactin gene.
  Hai Jin , Hong-Quan Chen , Jie Qin , Yin-Jian Zhu , Hua Chen , Gong-Wei Chen , Ya-Nan Xie , Zhong-Ting Pan , Ming-Hui Jiao , Sheng-Qiang Huang and Ming-Xing Chu
  Adenosine Monophosphate activated Protein Kinase (AMPK) plays a key role in cellular metabolism and regulating energy and its γ3 subunit was encoded by the PRKAG3 gene which was regarded as the candidate gene for carcass quality trait in domestic animals and fowls. To understand PRKAG3 gene variation and its association with carcass quality of goat breeds, the polymorphism of goat PRKAG3 gene and its distribution patterns in different goat breeds were investigated. Five goat breeds selected were Anhui white goat, Matou, Boer, Xiangdong and Sanen dairy goat. DNA samples of all goat breeds were extracted from goat ear tissue. The polymorphisms in the 5’ regulatory region and exon 13 of PRKAG3 gene were examined using ApaI, Hpy188I, AciI and ClaI enzyme digestion, respectively and their distribution patterns were analyzed in different goat breeds. The results showed that two mutation loci in the 5’ regulatory region, C-525A and C-225T, located at -525 and -225 bp upstream of the start codon of the PRKAG3 gene and two mutation loci in the exon 13, T90C and C102T, located at 90 bp and 102 bp of the exon 13. The 525CC genotype frequencies of five goat breeds listed above were 0.8000, 0.9681, 0.6042, 0.7557 and 0.8889, respectively, while 225CC genotype frequencies corresponding to 0.7391, 0.9750, 0.8684, 0.9785 and 0.9344. The mutations at T90C and C102T didn’t cause the substitution of corresponding amino acids in the AMPK protein. The muscle fat grade and carcass fat grade are lower in goat breeds with higher 525CC225CC genotype frequency while higher in the breeds with higher 525CA225CT genotype frequency (p<0.05). The results suggest that the lipoidosis ability of goat breeds may be associated with C-525A and C-225T loci of PRKAG3 gene.
  Hong-Quan Chen , Jie Qin , Yin-Jian Zhu , Zhong-Ting Pan , Ya-Nan Xie , Ming-Hui Jiao , Gong-Wei Chen , Hua Chen and Ming-Xing Chu
  THRSP gene has been regarded as a candidate gene for lipogenesis in domestic animals. To understand the association of THRSP gene with habitats and fatty deposits in goat breeds, the polymorphisms of goat THRSP gene, the fat content of goat carcass and their association with the ecological factors were investigated. The Chinese indigenous goat breeds selected were Guishan, Yunling, Chuandong, Banjiao, Xiangdong, Matou and Anhui white goat. The polymorphisms in the 5’ regulatory region and exon 1 of THRSP gene were analyzed using PCR-RFLP. The longissimus Muscle Fat Grade (MFG) and Carcass Fat Grade (CFG) of all goat breeds were estimated. The observed data of ecological factors were provided by the meteorological observatory in the goat habitats which included Average Daily Rainfall (ADR), Elevation (E), average Daily Sunshine Duration (DSD), Latitude (LAT) and longitude. E/LAT, ADRxDSD and SCxEC were regarded as Space Coefficient (SC), Energy Coefficient (EC) and Ecological Index (EI), respectively. The results showed that three mutational sites, C-233T, G113A and A138G, were detected. Nine genotypes in all goat breeds were only found, of which the frequencies of TCAAGG, TTGAAG and TTAAGG genotype were lower than 0.05. The EC significantly related to CAG haplotype frequency (0.8859, p<0.01) and the associations of EI with TGA haplotype frequency was significant (-0.8377, p<0.025). In the four haplotype frequencies, the relationship between CAG haplotype frequency and MFG was significant (-0.8633, p<0.025). The results suggest that the polymorphism of goat THRSP gene may be associated with the ecological factors and affect the goat lipogenesis ability.
 
 
 
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