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Articles by Jianfeng Sun
Total Records ( 3 ) for Jianfeng Sun
  Jianfeng Sun , Tianshu Yang , Xiongwei Zhao and Xinyue Li
  Objective: In this study the optimum processing conditions of instant shrimp of Litopenaeus vannamei was carried out. Methodology: The changes in texture properties, moisture content, salt content, amino acids and sensory evaluation were acted as analytic indexes, the effect of steaming time, formulation of flavoring liquid and the dipping time on product quality were investigated through single-factor test and orthogonal test. Results: After raw material cleaning, the materials were steamed 5 min, dipped in flavoring liquid for 1.5 h and then dried in infrared oven for 35 min. The best formulation of flavoring liquid was that the ratio between material and water is 1:3, salt 2%, sugar 3%, cooking wine 2%, white vinegar 0.2% and monosodium glutamate 0.1% for per 100 g water. The amino acid content of obtained instant shrimp is complete relatively and the total amount of amino acid is 349.527 mg g–1. Conclusion: The main results provide reference for the industrial production of instant shrimp.
  Shujing Meng , Zhili Xu , Xinpeng Dai , Jianfeng Sun and Zhuo Yang
  Background and Objective: Stalk of Lentinus edodes, which often discarded in large amounts after Lentinus edodes process is a kind of material that is rich in dietary fiber. The objective of study was to obtain a suitable way to extract Soluble Dietary Fiber (SDF) from the stalks of Lentinus edodes and provide theoretical basis for further processing of it. Methodology: According to single-factor and response surface methodologies, suitable conditions for extracting SDF were determined through enzymatic hydrolysis based on extraction yield of water-SDF and one-way-analysis of variance (ANOVA) followed by Least Significant Difference test (LSD) and Duncan’s multiple range test (Duncan) at p<0.05 were used to verify significant differences in evaluated parameters. Results: The optimal conditions for the SDF extraction rate are 52°C extraction temperature, 87 U g–1 enzyme concentration, 100 mesh number and 50 min extraction duration. On this condition, the SDF extraction rate is 14.33%. Water holding capacity and swelling property of SDF from stalks of Lentinus edodes measured 10 g/g and 0.93 mL g–1, respectively. Conclusion: It is concluded that stalks of Lentinus edodes serve as good sources of SDF. Reliable and feasible optimization of SDF extraction from stalks of Lentinus edodes can be achieved through response surface method.
  Jianlong Wang , Xiaozhe Zhou , Xiaowei Xing , Yanliang Chen , Gangwen Guo , Jianfeng Sun , Qingshan Zhang and Minren Shen
  Brain-Derived Neurotrophic Factor (BDNF) is a member of the neurotrophin family of growth factors and plays significant role in central nervous system and the peripheral nervous system for supporting the survival of existing neurons and encouraging the growth and differentiation of new neurons and synapses. Increasing study indicate that BDNF is associated with neurological disorders and psychiatric diseases. Beyond the importance of BDNF in nervous system, compelling evidence also demonstrate that it is also essential for body weight control and energy homeostasis. BDNF therefore, become the target in various diseases research such as Alzheimer’s disease, depressive disorder and obesity. In this study, researchers cloned the BDNF gene to p-EGFP N1 expression plasmid, meanwhile, researchers also transfected the p-EGFP N1-BDNF to Hela cell with lipofection. Fluorescence microscope and Western blot analysis showed BDNF could normally express in Hela cell. However, the physiological functions and pharmacological roles of the p-EGFP N1-BDNF need further validation in cellular level and animal model.
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