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Articles by Jian-Hua Wang
Total Records ( 3 ) for Jian-Hua Wang
  Chun-Hui Zhang , Yong-Lu Liu and Jian-Hua Wang
  A total of 51 pathogenic Escherichia coli isolates recovered from fowls and pigs were tested for production of Extended Spectrum β-Lactamases (ESBLs) by the Double Disc Synergy Test (DDST). For nine Enterobacteriaceaes tested for ESBLs by double-disk method we designed and synthetized two primer: TEM-1and SHV-1 studied ESBLs gene type with amplified, sequenced and analyzed by PCR; the Minimal Inhibitory Concentrations (MICs) of Cetiofur, Ceftriaxone, Amoxicillin, Ampicillin to the nine Enterobacteriaceae producing ESBLs were carried out with two fold dilution method. The ESBLs producing bacteria amplified TEM type ESBLs fully but no SHV type ESBLs, the Minimal Inhibitory Concentrations (MICs) of Cetiofur, Ceftriaxone, Amoxicillin and Ampicillin were 10-80 μg mL-1, 40-80 μg mL-1, >128 μg mL-1, >128 μg mL-1, respectively. This study reveals that E. coli isolated from chickens and swines in henan are resistant to the 3rd Cephalosporin Seriously and produce ESBL in large proportions.
  Chun-Hui Zhang , Xiao-Gen Zhang , Yong-Shu Shen and Jian-Hua Wang
  The multi-drug resistance of bacteria is a serious threat to the treatment of clinical infectious diseases; Escherichia coli (E. coli) possess plasmid-encoded Extended Spectrum β-Lactamases (ESBLs) that confer multi-drug resistance. In order to overcome this kind of resistance, some experiments were done in this study. In this study for the clinic pathogenic E. coli, ESBLs genetype were detected with PCR and for the induced E. coli, the plasmid elimination effects of several plasmid-curing agents to ESBLs were investigated using SDS as a control. Results showed that the bacteria producing ESBLs were amplified TEM type ESBLs fully, two strains were amplified both TEM type ESBLs and SHV type ESBLs, only one strain was amplified both TEM type ESBLs and CTX type ESBLs. And to the induced E. coli C83845-y, the elimination rate of chlorpromazine, hydrochloride, diazepam, norfloxacin and ciprofloxacin was 14.53, 13.50, 14.59, 8.43 and 8.23%, respectively, the elimination rate of SDS was 11.05%, blank control was 0.05% to induce E. coli C83907-y, the elimination rate of chlorpromazine, hydrochloride, diazepam, norfloxacin and ciprofloxacin was 11.25, 12.23, 13.51, 9.91 and 9.03%, respectively, the elimination rate of SDS was 10.72%, blank control was 0.05%. These results indicated that ESBLs-positive E. coli in Henan have transmitted resistant gene plasmids among different bacteria, the tricyclic-psychotropic drugs were more effective at eliminating the ESBL-resistant plasmid. This study provides insight into strategies for overcoming the resistance conferred by ESBLs.
  Guo-Dong Yang , Dan-Ju Kang , Yan-Hong Li , Jin-Cheng Li , Yan Wang , Xiang-Ya Kong , Qin-Fan Li and Jian-Hua Wang
  Endophytic Undifilum oxytropis found within locoweeds produces the poisonous alkaloid swainsonine which is responsible for locoism in grazing animals. A modified enzymatic assay for swainsonine in the mycelia of Undifilum fungi was developed. The standard equation was Y = 0.5169X- 0.1129 (regression coefficient r = 0.9985). The calibration graph of swainsonine was linear in the range 0.05-0.5 μg mL-1. The method was validated for linearity, precision and accuracy. The modified method has been applied to explore the swainsonine accumulation properties in the endophytic Undifilum fungi in liquid media. Under the described culture condition, the mycelia weight reached a maximum value on the 20th day whereas the peak of swainsonine biosynthesis took place on the 28th day. The result indicated that the increment of mycelium weight and the swainsonine accumulation in the endophytic Undifilum fungi is not completely synchronous.
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