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Articles by Jian Luo
Total Records ( 3 ) for Jian Luo
  Erick Cardenas , Wei-Min Wu , Mary Beth Leigh , Jack Carley , Sue Carroll , Terry Gentry , Jian Luo , David Watson , Baohua Gu , Matthew Ginder-Vogel , Peter K. Kitanidis , Philip M. Jardine , Jizhong Zhou , Craig S. Criddle , Terence L. Marsh and James M. Tiedje
  Microbial enumeration, 16S rRNA gene clone libraries, and chemical analysis were used to evaluate the in situ biological reduction and immobilization of uranium(VI) in a long-term experiment (more than 2 years) conducted at a highly uranium-contaminated site (up to 60 mg/liter and 800 mg/kg solids) of the U.S. Department of Energy in Oak Ridge, TN. Bioreduction was achieved by conditioning groundwater above ground and then stimulating growth of denitrifying, Fe(III)-reducing, and sulfate-reducing bacteria in situ through weekly injection of ethanol into the subsurface. After nearly 2 years of intermittent injection of ethanol, aqueous U levels fell below the U.S. Environmental Protection Agency maximum contaminant level for drinking water and groundwater (<30 µg/liter or 0.126 µM). Sediment microbial communities from the treatment zone were compared with those from a control well without biostimulation. Most-probable-number estimations indicated that microorganisms implicated in bioremediation accumulated in the sediments of the treatment zone but were either absent or in very low numbers in an untreated control area. Organisms belonging to genera known to include U(VI) reducers were detected, including Desulfovibrio, Geobacter, Anaeromyxobacter, Desulfosporosinus, and Acidovorax spp. The predominant sulfate-reducing bacterial species were Desulfovibrio spp., while the iron reducers were represented by Ferribacterium spp. and Geothrix spp. Diversity-based clustering revealed differences between treated and untreated zones and also within samples of the treated area. Spatial differences in community structure within the treatment zone were likely related to the hydraulic pathway and to electron donor metabolism during biostimulation.
  Jian Luo , Liu Yu , Dafang Zhang , Zhen Xia and Wei Chen
  Efficient, accurate, real-time and stable link quality estimation is essential to guarantee the upper layer protocol performance in wireless sensor network. This study expounds a new LQI-based (Based on Link Quality Indicator) link quality estimation mechanism for wireless sensor networks, which takes the lost packets and error packets into account. Based on real platform, we carry out a large number of experiments. After being analyzed the collected data, a new link evaluation model between LQI and PRR (Packet Received Ratio) is established. Experiment results have validated the correctness of the LQI-PRR model and the results also show that compared with traditional assessment methods, the mechanism proposed in this study improves the accuracy of the assessment and is more useful. At last, we analyze BER (Bit Error Ratio) and draw a relationship between LQI-PER (Packet Error Ratio), LQI-BER in order to get a further understands of the link quality.
  Huiping Song , Jian Luo , Weijia Luo , Jinsheng Weng , Zhiqiang Wang , Baoxing Li , Dali Li and Mingyao Liu
  G-protein-coupled receptors (GPCRs), one of the most versatile groups of cell surface receptors, can recognize specific ligands from neural, hormonal, and paracrine organs and regulate cell growth, proliferation, and differentiation. Gpr48/LGR4 is a recently identified orphan GPCR with unknown functions. To reveal the functions of Gpr48 in vivo, we generated Gpr48-/- mice and found that Gpr48-/- fetuses displayed transient anemia during midgestation and abnormal definitive erythropoiesis. The dramatic decrease of definitive erythroid precursors (Ter119pos population) in Gpr48-/- fetal liver at E13.5 was confirmed by histological analysis and blood smear assays. Real-time PCR analyses showed that in Gpr48-/- mice both adult hemoglobin α and β chains were decreased while embryonic hemoglobin chains (ζ, βH1, and εy) were increased, providing another evidence for the impairment of definitive erythropoiesis. Furthermore, proliferation was suppressed in Gpr48-/- fetal liver with decreased c-Myc and cyclin D1 expression, whereas apoptosis was unaffected. ATF4, a key transcription factor in erythropoiesis, was down-regulated in Gpr48-/- fetal livers during midgestation stage through the cAMP-PKA-CREB pathway, suggesting that Gpr48 regulated definitive erythropoiesis through ATF4-mediated definitive erythropoiesis.
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