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Articles by Jia Yao
Total Records ( 6 ) for Jia Yao
  Jianbo Yang , Jia Yao , Kun Yang , Zichun Hua and Jie Yang
  The gene sequence of Decorsin which is extracted from a kind of North American leeches was synthesized. Two recombinant proteins, Annexin V plus Decorsin (AnnV-D39) and Annexin V plus the carboxyl terminal 27 amino acid residues of Decorsin(AnnV-D27), were constructed. And a 10 amino acids linker peptide of GGGGSGGGGS was inserted between Annexin V and Decorsin in AnnV-D39. Using pET-28(a+) as an expressing vector, both two recombinant proteins were expressed in E. Coli BL21(DE3) with high efficiency as inclusion bodies. The expression products were purified by DEAE-Cellulose 52 and Sepharose CL-4B chromatography under denaturing condition. Platelet Aggregation Assay (PAA) shows that AnnV-D39 has good anti-platelet aggregation activity. However, AnnV-D27 shows no such activities in any PAA test. AnnV-D39 shows good anti-platelet aggregation activity as a new antagonist of fibrinogen receptor, while Annv-D27 needs re-modification.
  Jia Yao , Kun Liao , Qiu-Ling Li , Chang-Fa Wang , Hong-Mei Wang , Song-Jia Lai and Ji-Feng Zhong
  High ambient temperature has largely reduced the milk production due to the weak heat tolerance ability of Holstein. One potential mechanism for the destructive effect on organism is that the heat stress stimulated excessive cellular toxicants. The glutathione S-transferase Pi (GSTP1) has been proposed to play an important role to inactivate toxic metabolites in human malignant tumors. In this study, researchers evaluated the effect of heat stress on GSTP1 mRNA expression in Holstein using semi-quantitative RT-PCR method. With the liver tissue exception, the GSTP1 gene showed relatively high expression level in heart, spleen and kidney tissues in cool ambient temperature. After heat stress treatment, the GSTP1 mRNA expression increased significantly in all studied tissues. We sequenced the 3705 bp fragment containing complete sequence of GSTP1 gene among 15 cows and detected 31 variations. The nonsynonymous variation of G18C (p.M6I) was further scanned in 106 Holsteins using RFLP method to analyze the association of genotypes with heat tolerance ability. However, we did not detect statistical difference of heat tolerance ability among genotypes. To the knowledge, this is the first report to study GSTP1 mRNA expression under heat stress, SNPs distribution and association of genotypes with heat tolerance ability in Holstein. The significantly elevated expression of GSTP1 would suggest the positive role to resist heat stress, especially in liver tissue.
  Jia Yao , Ming Zhang , Yu-Mei Feng , Si-Yi Chen , Wei Wang , Jie Wang and Song-Jia Lai
  Interferon-tau (IFN-τ) secreted by blastular trophectoderm was thought to be the primary signal for pregnancy recognition during bovine early pregnancy period after pregnancy recognition, progesterone dominated pregnancy maintaining. Moreover, Matrix Metalloproteinases-2 (MMP-2) and Tissue Inhibitor of Metalloproteinases-2 (TIMP-2) was important for embryo implantation. The objectives of studies were evaluated whether IFN-τ and progesterone regulated MMP-2 and TIMP-2 expression in cultured bovine Endometrial Cells (bECs). In the studies, bECs were cultured in DMEM-Ham’s F12 medium with free-serum. These cultured cells were divided into 4 groups and cultured in four types culture medium: culture medium without IFN-τ and progesterone (Co group); culture medium including 100 ng mL-1 IFN-τ (IN group); culture medium including 200 nmol L-1 progesterone (Pr group); culture medium including 100 ng mL-1 IFN-τ and 200 nmol L-1 progesterone (I+P group). Cultured bECs were harvested and extracted total mRNA after being cultured for 1, 3, 6 and 12 h and then expression level of MMP-2 and TIMP-2 were assessed with fluorescence quantitative PCR. The results showed that MMP-2 expression level in Pr, IN and I+P group were extremely significantly higher than in Co group (p<0.01). MMP-2 expression level in IN and I+P group was significantly higher than in Pr group (p<0.05). MMP-2 expression level was no statistical difference between IN group and I+P group (p>0.05); TIMP-2 expression level in Pr, IN and I+P group was extremely significantly lower than in Co group (p<0.01) and TIMP-2 expression level was no statistical difference among Pr, IN and I+P group (p>0.05). The conclusion indicated progesterone and IFN-τ induced MMP-2 expression in vitro cultured bECs whereas, progesterone and IFN-τ suppressed TIMP-2 expression in vitro cultured bECs.
  Ying-He Qin , Wei Wang , Jia Yao , Jian Wang , Xiao-Mao Guo and Song-Jia Lai
  Meat flavor, one of the most important characteristic of meat quality in farm animals is significantly determined by the Inosine Monophosphate Acid (IMP) content. However, the genetic regulation underlying IMP synthesis has largely remained unknown at the present. In this study, researchers cloned the AMPD1 gene in rabbit and investigated the mRNA expression in longissimus dorsi muscle at 40, 70 and 90 days old, respectively using real-time PCR method. The association between mRNA expression and IMP content was subsequently studied among 48 individuals from two breeds of Tianfu black rabbit (n = 24) and Harbin albino rabbit (n = 24). Total 899 bp fragment containing entire coding region was obtained for rabbit AMPD1 gene. There is no significant difference of IMP content between the two breeds rabbits in the three studied ages (p>0.05). The slaughter age has significant effect on the IMP content with the highest value at 90 days old (3.41±0.29) and the lowest values at 40 days old (2.50±0.39). Researchers detected the negative correlation between IMP content and AMPD1 mRNA expression (r = -0.916, p<0.05). The AMPD1 gene have not significant effects on IMP contents. The results suggested the potential function of AMPD1 to determine the IMP content in rabbit which is significant to conduct the MAS for improving the meat quality.
  Wei Li , Ya-Hu Gao , Min Chang , Ya-Li Peng , Jia Yao , Ren-Wen Han and Rui Wang
  Neuropeptide S (NPS), a recently identified bioactive peptide, was reported to regulate arousal, anxiety, motoring and feeding behaviors. NPS precursor and NPS receptor mRNA were found in the amygdala, the ventral tegmental area (VTA) and the substantia nigra, the area thought to modulate rewarding properties of drugs. In the present study, we examined the influence of NPS on the rewarding action of morphine, using the unbiased conditioned place preference (CPP) paradigm. Morphine (1, 3 and 6nmol, i.c.v.) induced a significant place preference. For testing the effect of NPS on the acquisition of morphine CPP, mice were given the combination of NPS and morphine on the conditioning days, and without drug treatment on the followed test day. To study the effect of NPS on the expression of morphine CPP, mice received the treatment of saline/morphine on the conditioning days, and NPS on the test day, 15min before the placement in the CPP apparatus. Our results showed that NPS (0.3–10nmol) alone neither induced place preference nor aversion, however, NPS (1 and 3nmol) blocked the acquisition of CPP induced by 3nmol morphine, and acquisition of 6nmol morphine-induced CPP was also reduced by NPS (6 and 10nmol). Moreover, the expression of CPP induced by 6nmol morphine was also inhibited by NPS (0.1, 1 and 10nmol). These results revealed the involvement of NPS in rewarding activities of morphine, and demonstrated the interaction between NPS system and opioid system for the first time.
  Jia Yao and Sandra M. Bajjalieh
  Synaptic vesicle protein 2 (SV2) is required for normal calcium-regulated secretion of hormones and neurotransmitters. Neurons lacking the two most widely expressed isoforms, SV2A and SV2B, have a reduced readily releasable pool of synaptic vesicles, indicating that SV2 contributes to vesicle priming. The presence of putative ATP-binding sites in SV2 suggested that SV2 might be an ATP-binding protein. To explore this, we examined the binding of the photoaffinity reagent 8-azido-ATP[γ] biotin to purified, recombinant SV2 in the presence and absence of other nucleotides. Our results indicate that SV2A and SV2B bind nucleotides, with the highest affinity for adenine-containing nucleotides. SV2A contains two binding sites located in the cytoplasmic domains preceding the first and seventh transmembrane domains. These results suggest that SV2-mediated vesicle priming could be regulated by adenine nucleotides, which might provide a link between cellular energy levels and regulated secretion.
 
 
 
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