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Articles by Jean De Dieu Tamokou
Total Records ( 2 ) for Jean De Dieu Tamokou
  Leopold Havyarimana , Simplice Tatsimo Ndendoung , Jean de Dieu Tamokou , Alex de Theodore Atchade and Joseph Mbafor Tanyi
  Context: Millettia barteri (Benth.) Dunn (Fabaceae) is an African medicinal plant used in folk medicine to treat many diseases. This species, as well as other Mellettia species, has been of interest to researchers because of their wide range of traditional uses. Objective: Phytochemical, antimicrobial and 2,2-diphenyl-1-picryl-hydrazyl-hydrate (DPPH)-radical scavenging investigations of the hexane and EtOAc extracts of the stem bark of M. barteri were carried out here for the first time. Materials and methods: The isolation of compounds was done through silica gel column chromatography and their structures were established using spectroscopic analysis, especially, 1D NMR in conjunction with 2D experiments (COSY, HMQC and HMBC), and physical data compared with literature values. The broth micro dilution method was used for antimicrobial test while DPPH radical scavenging assay was used for antioxidant test. Results: Seven compounds, including two guanidine alkaloids: millaurine (2) and milletonine (7); one flavonoid: afzelin (6); four sterols: β-sitosterol (1), β-sitosterol glucoside (3), mixture of stigmasterol (4) and β-sitosterol (5) palmitates have been isolated from stem bark of hexane and ethyl acetate extracts of M. barteri. These extracts showed antimicrobial activity on the set of germs tested with minimum inhibitory concentration values varying from 64 to 512 μg/mL, as well as antioxidant activity (IC50 62.74 and 77.23 μg/mL). Compounds 2 and 7, tested for the first time, demonstrated antimicrobial and antioxidant activities. Discussion and conclusions: The present study clearly demonstrated that M. barteri and some of its isolates possess antimicrobial and antioxidant properties and may act as potential antioxidant for biological systems susceptible to free radical-mediated reactions.
  Raymond S. Mouokeu , Rosalie A.N. Ngono , Christopher Tume , Monique O. Kamtchueng , Guy S.S. Njateng , Dzoyem Jean Paul , Jean De Dieu Tamokou and Jules-Roger Kuiate
  Medicinal plants are rich sources of secondary metabolites which are claimed to possess immunomodulatory properties, justifying why the present study was designed to investigate the immunomodulatory potential of Crassocephalum bauchiense leaves extracts. Leaves were macerated successively in hexane, ethyl acetate and methanol. The ethyl acetate extract was further fractionated into six fractions (F1 to F6) using flash chromatography. The activities of the extract and its fractions were investigated on macrophage NO production model while their proliferative effects were evaluated on macrophages and peripheral blood lymphocytes by the thiazolyl blue (MTT) test. Extract and fractions significantly inhibited NO production by peritoneal macrophages. However, they enhanced macrophage and lymphocyte proliferation in vitro at lower concentrations (<64 or <128 μg mL-1). Fraction F2 significantly increased Delayed-type herpersensitivity reaction (DTH) in mice. This activity was potentiated when the fraction was co-administered with cyclophosphamide. The fraction did not show any activity on antibody production. The total leucocytes count (mainly lymphocytes) was higher in the extract-treated groups when compared to the controls. Fraction F2 significantly suppressed myelosuppression induced by cyclophosphamide as from 25 mg kg-1 of body weight (bw). Oral administration of the fraction caused lethal signs of toxicity from 16 mg kg-1 bw. The LD50 was estimated at 22 mg kg-1 bw. These results indicate that C. bauchiense ethyl acetate extract and its fraction F2 have relatively interesting immunostimulatory effects on lymphocytes. In associated with its kwon antimicrobial properties, one can conclude that this plant species is a candidate for anti-infective phytomedicine preparation.
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