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Articles by Jay C. Means
Total Records ( 2 ) for Jay C. Means
  Worlanyo E. Gato and Jay C. Means
  To assess DNA damage from exposure to 2-Aminoanthracene (2AA), Single Cell Gel Electrophoresis (SGCE) was employed to examine single strand breaks in blood of Fisher-344 rats. Although subcellular injury due to 2-AA exposure has been noted in the past, there is yet to be a direct demonstration of genetic alterations due to 2-AA intoxication. In the current study, alkaline comet assay was used to evaluate the type of DNA damage that is on-going in the blood samples of F-344 animals. The animals were fed control (0 mg kg-1), LD (50 mg kg-1), MD (75 mg kg-1) and HD (100 mg kg-1) 2-AA diet for two and four weeks. At the end of each exposure period (14 or 28 days), rats were euthanized with CO2 and blood was collected by cardiac puncture. Twenty micro liters of whole blood samples were added to 1 mL of Hanks Balanced Salt Solution (HBSS without Ca2+ and Mg2+) and subsequently snapped frozen in liquid nitrogen. Following comet assay analysis, single strand break were assessed via tail moment, tail length, tail intensity and head intensity scoring of comets along with cell viability. There seems to be an apparent dose response. That is 50, 75 and 100 mg kg-1 diet 2-AA rats when compared with the control demonstrated significant (p<0.05) damage as measured by tail length and tail moment values. Head and tail intensity values for intoxicated animals were also significant at 2 and 4 weeks relative to the controls, although there were no dramatic shifts between exposure time.
  Worlanyo E. Gato and Jay C. Means
  To understand the mechanism of arylamine toxicity, the effect of 2-Aminoanthracene (2-AA) on the pancreas of Fisher-344 rats was investigated. Twenty four post-weaning 3-4 week old F-344 male rats were exposed to 0 mg kg-1 diet (control), 50 mg kg-1 diet (low dose), 75 mg kg-1 diet (medium dose) and 100 mg kg-1 diet (high dose) 2-AA for 14 and 28 days. This was followed by analysis of the pancreas for global gene expression changes by Affymetrix Microarray GeneChips. More Gene Ontology (GO) categories were found to be significantly altered for the 14 day study than the 28 day study. In the 14 day study, 45, 57 and 237 cellular component, molecular function and biological process gene ontology categories were found to be significant, respectively. Similarly, 7, 5 and 25 cellular component, molecular function and biological process GO categories were altered, respectively. Some of these GO categories include; modification-dependent protein catabolic process, phospholipid biosynthetic process and glucose. VisANT was employed to analyze the dataset and to explore the biological network relationship between differentially expressed genes. This software was employed to analyze the dataset and to explore the relationships between differentially expressed genes. Three mRNA transcripts were identified from the network plot to have at least 50 links with other genes. These include SLC2A4 (glucose transporting gene), MAPK3 (a signal transduction pathway protein, mitogen activated protein kinase 3 and RAD23B (DNA repair protein and ubiquitin-like containing protein). The current study identified altered gene expression profiles associated with pancreatic carcinoma, pancreatitis and/or type 2 diabetes. This may be useful to identify and develop biomarkers as a diagnostic tool associated with the onset of these pathologies.
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