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Articles by Jay Prakash Verma
Total Records ( 5 ) for Jay Prakash Verma
  Janardan Yadav , Jay Prakash Verma and Kavindra Nath Tiwari
  Aspergillus niger strain BHUAS01, Penicillium citrinum strain BHUPC01 and Trichoderma harzianum was tested for phosphate solubilizing potential and plant hormones production (indole acetic acid). The fungal strains were tested for their in-vitro phosphate solubilizing potential using Pikovskaya broth containing tricalcium phosphate (TCP) as the sole phosphorus source. All the cultures were found to solubilize TCP but with varying potential. The Aspergillus niger showed maximum amount of soluble phosphate (328 μg mL-1), followed by P. citrinum (301 μg mL-1) and T. harzianum (287 μg mL-1) after 6 days of incubation at 28±2°C. Indole Acetic Acid (IAA) production was estimated maximum in A. niger (85 μg mL-1) followed by T. harzianum (68 μg mL-1) and P. citrinum (52 μg mL-1) after 3 days of incubation at 28+2°C. T. harzianum is a well known biological controlling agent against several soil borne phytopathogens. It showed antagonistic against Fusarium oxysporum and Rhizoctonia solani. Under laboratory conditions, coinoculation of T. harzianum and A. niger showed significant increase in chickpea (Cicer arietinum) growth parameters including shoot length, root length and dry weight of shoot and root followed by single inoculation of T. harzianum and coinoculation of A. niger and P. citrinum over control. The study explores high P-solubilizing potential of A. niger and T. harzianum, which can be exploited for the solubilization of fixed phosphates present in the soil and produce IAA, thereby enhancing soil fertility and plant growth. Coinoculation of T. harzianum and A. niger could be effective biofertilizer and biocontroling agent for chickpea production.
  Lavakush , Janardan Yadav and Jay Prakash Verma
  The Plant Growth Promoting Rhizobacteria (PGPR) can substantially reduce the chemical inputs in agriculture. Furthermore, the use of indigenous PGPR can be an added advantage since it can easily acclimatize to the natural conditions and enhanced the plant–microbe interactions. The objective of my research is screening, characterization and selection of effective PGPR for rice production. Eight efficient PGPR isolates were selected and identified as Pseudomonas aeruginosa strain BHUJY12 (HQ-236532), Pseudomonas putida strain BHUJY14 (HQ-236533), P. aeruginosa strain BHUJY16 (HQ-236535), Pseudomonas sp. strain BHUJY19 (HQ-236519), P. aeruginosa strain BHUJY22 (HQ-236541), P. putida strain BHUJY23 (HQ-236542), P. aeruginosa strain BHUJY24 (HQ-236543) and P. aeruginosa strain BHUJY25 (HQ-236544). The bacterial strains were recorded range from 115.94 to 228.71 μg mL-1 phosphate solubilization in Pikovskaya broth medium. Also pH of the medium was ranged from 6.77 to 4.65 and SI index of different isolates ranged from 2.28 to 4.23 on solid agar plate at 6 days of incubation. Indole-3-acetic acid (IAA) production was varied range from 12.19 to 22.91 μg mL-1 at 6 days incubation. PGPR strains were also showed the growth inhibition of Rhizoctonia solani at 3 and 6 days incubation. Among all the strains, BHUJY23 strain (Pseudomonas putida) was found maximum significant phosphorus solubilization and IAA production followed by strain BHUJY16. Therefore, the P. putida strain BHUJY23 was effective PGPR for rice production under Indo-Gangetic plain of Eastern Uttar Pradesh.
  Jay Prakash Verma , Janardan Yadav , Kavindra Nath Tiwari , Lavakush and Vimal Singh
  Rhizospheric soils of crop plants have more flora and fauna due to availability of more organic compound, macronutrient and micronutrient. Rhizobacteria that exert beneficial effects on plant growth and development are referred to as Plant Growth Promoting Rhizobacteria (PGPR). Plant growth promoting rhizobacteria is a group of free living soil bacteria, which have ability to promote growth and yield of crop plant by direct and indirect mechanism. PGPR is generally two type, one is colonies inside plant cells that called intracellular PGPR (iPGPR) and other colonies out side plant in rhizosphere that called extracellular PGPR (ePGPR). This review generally focused on direct and indirect mechanism of PGPR. Direct mechanism of plant growth promotion may involve the synthesis of substances by the bacterium or facilitation of the uptake of nutrients from the environment. The indirect mechanism of plant growth occurs when PGPR lessen or prevent the deleterious effects of plant pathogens on plants by production of inhibitory substances or by increasing the natural resistance of the host. The search for PGPR and investigation of their mode of action are increasing at a rapid use as commercial biofertilizers. The mode of action and practical application of PGPR in crop production are the major focus of this review.
  Jay Prakash Verma , Vimal Singh and Janardan Yadav
  The aim of this study was to find the effect of copper as a micronutrient for enhanced plant growth, protein content and antioxidant enzyme activity of mungbean (Vigna radiata) under the influence of different concentrations of copper. The effect of various copper sulphate solution has insignificant effect on the percent germination of mungbean while plumule and radicle length decreased with increase in copper concentration (50, 200, 500 and 1000 μM copper sulphate solution). Total protein content in root was significantly higher (127.7%) than shoot (83.7%) as compared to control. Peroxidase activity, in case of shoot, increases with increase in copper sulphate concentration i.e., (1.423 of control to 1.713, 2.29, 2.52, 2.88 and 3.02 OD/min/mg protein in different copper sulphate solutions treatments after 72 h) while for the root copper sulphate solution has negative effect on the peroxidase activity i.e., (11.41 of control to 11.19, 10.85, 10.04, 9.73 and 9.40 OD/min/mg protein in different copper sulphate solutions treatments after 72 h). The present experiment revealed that copper is an essential micronutrient which promoted the seedlings growth at less than 50 μM concentration. Higher concentration significantly decreases shoot and root length. The protein concentration in both shoot and root of the seedling was recorded to be enhanced with increase in the concentration of copper sulphate as compared to control. POD activity in root decreased at higher levels of copper concentration while POD activity in shoot increased with increasing concentration.
  Janardan Yadav , Jay Prakash Verma and Kavindra Nath Tiwari
  Plant Growth Promoting Fungus (PGPF) isolated from rhizosphere of chickpea and identified as Aspergillus niger strain BHUAS01 was tested for its tricalcium phosphate solubilizing ability at different sources of carbon viz., glucose, sucrose, glycerol and mannitol in Pikovskaya broth. Also, A. niger was analyzed solubilization of tricalcium phosphate (TCP) in broth media at different salinity viz., 1% NaCl, 1% KCl and 1% CaCl2 at varying range of reaction (pH) under in vitro condition. Among the carbon sources, Aspergillus niger was found to solubilize maximum tricalcium phosphate (512 μg mL-1) at glucose as carbon source and minimum activity (348 μg mL-1) of phosphate solubilization at sucrose as carbon. Aspergillus niger showed maximum significant solubilization of tricalcium phosphate in Pikovaskaya broth containing carbon source glucose followed by glycerol, maltose and sucrose at 21 days of incubation. Further the effect of different salinity (1% NaCl, 1% KCl and 1% CaCl2) was tested at different pH (6.0, 7.0 and 8.0) under in vitro condition. A. niger strain BHUAS01 was showed maximum significant solubilization of tricalcium phosphate (495 μg mL-1) in presence of 1% CaCl2 in modified Pikovskaya broth at pH 8.0 than other salt concentration. This finding can provide great benefit in the maintaining the available phosphates for crops in saline and alkaline soils. A large fraction of land arid and semiarid regions is affected by salinity in India.
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