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Articles by Jaswandi
Total Records ( 2 ) for Jaswandi
  Sri Melia , Endang Purwati , Yuherman , Jaswandi , Salam N. Aritonang and Mangatas Silaen
  Background and Objective: Listeria monocytogenes is an important pathogenic bacteria in various cases of poisoning in the food industry due to its ability to grow in cold temperatures and to survive in freezing temperatures. Lactic acid bacteria have important probiotic attributes including their antimicrobial effect against this pathogen. Therefore, this study aimed to isolate lactic acid bacteria from buffalo milk and characterize its antimicrobial activity against Listeria monocytogenes. Materials and Methods: Buffalo milk was collected from four districts in West Sumatera, Indonesia and its composition analysed. A total of 88 lactic acid bacteria strains were isolated and grown at De Man Rogosa Sharpe Agar (MRSA). The strains were identified based on morphology (shape, size and colour) and their biochemical characteristics (catalase test and the fermentation type) and then screened for antimicrobial activity against L. monocytogenes. The species were further identified based on 16S rRNA gene sequence analysis. Results: As a result of isolation and identification, 19 strains of lactic acid bacteria were screened against L. monocytogenes, but only three isolates (A 3.2, A 3.3 and TD 7.2) showed high inhibition against L. monocytogenes. They were identified using 16S rRNA gene sequence analysis. Conclusion: The BLAST results of the identification procedure showed that the isolated bacteria from buffalo milk belonged to Lactobacillus fermentum strain L 23 (A 3.3), Lactobacillus fermentum strain 6704 (TD 7.2) and Lactobacillus oris strain J-1 (A 3.2).
  Hermon , Jaswandi and F. Jhon
  Background and Objective: The aim of this experiment was to examine the supplementation of cassava leaves in a cow diet utilizing ammoniated rice straw and synchronized the release of N-protein and energy in the rumen to obtain the best digestion nutrients and the best urine allantoin concentration, plasma progesterone and BUN (blood urea nitrogen) level in the blood of the cow. Materials and Methods: By a randomized block design of a 2×3 factorial, two diet treatments, either without (R1) or with the cassava leaves supplementation (R2), were offered to twelve cows of different breeds, namely, the local cow (Pesisir cow) and Bali and Simmental cows. Results: There was no significant effect of the interaction between the breed and diet treatments on all of the parameters. Even so, the R2 diet showed a relatively higher nutrient digestion and feed efficiency. It not only had a relatively lower BUN level but also had a lower urine allantoin concentration compared to that of R1, whereas the BUN level of the Bali and Pesisir cows was higher than that of Simmental cow (p<0.05). The Simmental cow, however, showed a relatively higher feed efficiency and allantoin concentration in the urine and a relatively lower nutrient digestion and BUN level than those of the Pesisir and Bali cows. Conclusion: It is concluded that the supplement of 3.0% cassava leaves in the cow diet with ammoniated rice straw, having a synchrony index of 0.567, produced a good production performance and had no effect on the plasma progesterone level in the cow. Moreover, the highest plasma progesterone level was in the Bali cow followed by the Pesisir cow and Simmental cow.
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