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Articles by Jamsari Jamsari
Total Records ( 6 ) for Jamsari Jamsari
  Siti Nur Aisyah , Sulastri Sulastri , Retmi Retmi , Rahmi Henda Yani , Elly Syafriani , Lily Syukriani , Fatchiyah Fatchiyah , Amri Bakhtiar and Jamsari Jamsari
  Background and Objective: Plant-associated bacteria, such as Phyllobacterium play a significant role in protecting plant from pathogenic fungal infection. These Phyllobacterium are known to be existed in such a microbial community with various microbes thus leading to elevated disease suppression. The aim of this study was to assess the fungal suppression activity of indigenous Phyllobacterium isolates and its strain/species compatibility with rhizobacteria. Materials and Methods: Two indigenous Phyllobacterium isolates were identified using 16S rRNA gene sequence and its antifungal activities were tested against several phytopathogenic fungus. Further antagonistic assay was performed to compare the efficacy of cell culture and cell-free supernatants. Its compatibility was assayed by performing the antifungal assay using the combination of these Phyllobacterium isolates with rhizobacteria ones. Data were statistically analyzed using one-way analysis of variance and the significance was further processed using Duncan’s new multiple range test with a p<0.05. Results: Both isolates (UBCF_01 and UBCF_13), identified as Serratia plymuthica, exhibited higher suppression activity against Colletotrichum gloeosporioides compared to Fusarium oxysporum and Sclerotium rolfsii. Both isolates revealed opposite trend in their activities resulted from cultured cells and cell-free supernatants. Furthermore, the better suppression efficacy of the culture supernatants was resulted from single cultured cells, instead of co-culture. However, both isolates displayed quite poor compatibility with rhizobacteria isolates. Conclusion: These indigenous Phyllobacterium showed promising ability to be used as biocontrol agents for anthracnose. The application of its culture supernatants offered the less hazardous option of biological control implementation. However, their poor compatibility, even with the same species (rhizospheric UBCR_12) might be occurred due to habitat differences.
  Dessy Arisanty , Yanwirasti , Jamsari Jamsari , Wirsma A. Harahap and Daan Khambri
  Background and Objective: miR-10b is one of oncogenic miRNA first described in promoting metastases in Breast Cancer. The aim of the study was to investigate the expression of miR-10b and the expression of metastases-induced genes in BC (BC) and fibroadenoma (FA) in West Sumatra. Materials and Method: LNATM primer enhancer set was used to identify the miR-10b expression as a relative quantification. miR-16 was used as an endogenous control with a relative median expression of miR-10b at 2–ΔΔCt. The expression of metastases-related genes was performed by an absolute quantification method. Results: The statistical differences between miR-10b and the expression of genes were determined by t-student test to interpret the expression of BC and FA tissue (p<0.005). The expression of miR-10b in BC was lower than FA (endogenous control). Relative median of miR-10b expression in BC was 8.51 times lower than FA. Low expression of miR-10b in BC was associated with tissue grading. The expression of metastases-related gene; RhoC, TIMP2 and MMP2 were lower in BC than FA. miR-10b expression differed between BC and FA. Conclusion: RhoC, TIMP2 and MMP2 expression caused cancer cell whether to be metastasized or not. miR-10b is a potential marker to predict BC cell which start to be aggressive.
  Siti Nur Aisyah , Hafid Harnas , Sulastri Sulastri , Retmi Retmi , Helmi Fuaddi , Fatchiyah Fatchiyah , Amri Bakhtiar and Jamsari Jamsari
  Background and Objective: A new rhizobacteria isolate of Serratia plymuthica (strain UBCR_12) exhibited a promising potential as a biocontrol agent for anthracnose causing agent Colletotrichum gloeosporioides. The aim of this study was to characterize its antagonistic activity and explore the factors contributing to a higher inhibition activity. Materials and Methods: The antifungal effect of UBCR_12 against C. gloeosporioides was assayed under various pH values and nutritional sources. Culture supernatant obtained from UBCR_12 and C. gloeosporioides co-culture was also tested for its inhibitory activity. In addition, the antagonistic range of this isolate was examined against Sclerotium rolfsii and Fusarium oxysporum. Statistical analysis was done using one way analysis of variance and further processed using Fisher’s Least Significant Difference (LSD) test with a p<0.05. Results: The UBCR_12 induced inhibition was shown to be stable over time at pH 7, while peptone addition led to a faster induction (2 days after treatment) and glucose treatment to a higher activity. Of all these modifications, preliminary co-culture experiments with fungal cells resulted in the best antagonistic activity of UBCR_12 culture supernatant of about 30.66%. This isolate also showed a wide range of antagonistic activity due to its high suppression against S. rolfsii and F. oxysporum from soybean. Conclusion: Both environmental and biotic manipulations contributed an elevated inhibition rate of UBCR_12 against C. gloeosporioides. A proportional combination of the factors stimulating antagonistic activity of this strain is recommended to be utilized for the development of this strain as an antianthracnose. The enhanced antifungal effects of UBCR_12 resulted under each type of modification were varied indicating the difference of cell responses. It suggests that certain antifungal mechanism could be generated by modifying the environmental factor required for its induction. In addition, the application of cell-free culture supernatant provides an alternative solution in the utilization of biocontrol agents. For large scale application, it could minimize the risk of population outbreaks and harmful effects due to the living cells application.
  Susmiati , Nur Indrawaty Lipoeto , Ingrid S. Surono and Jamsari Jamsari
  Background and Objective: There are pros and cons surrounding the relationship between fat mass obesity associated (FTO) rs9939609 variants and the occurrence of obesity with regards to ethnicity and race. The Minangkabau ethnicity is unique compared to other ethnicities in Indonesia regarding its dietary pattern, in that this ethnic diet is high in fat intake and low in fibre intake. This study aimed to investigate the relationship between FTO rs9939609 variants and eating habits and food preferences among adolescent girls of Minangkabau ethnicity. Methodology: This study was a case control study and 275 adolescent girls (130 obese and 145 normal) were included. Tetra-primer amplification refractory mutation system-polymerase chain reaction (tetra-ARMS-PCR) was employed to examine genetic variants of FTO rs9939609. Eating habits were determined using an eating habits questionnaire and body mass index (BMI) was computed using the BMI Z-score (WHO). Results: This study revealed a significant relationship between genetic variants of FTO rs9939609 (TT, TA and AA genotypes) and higher BMI (p = 0.01). Those with the A allele were found to consume more fried food and have a lower intake of fruit (p<0.05) than those with the TT genotype. In the obese group, subjects with the A allele did not have a preference for a fruit-vegetable diet (p<0.05). Based on cooking method, subjects with the A allele preferred to eat less meat curry than those with TA and TT genotypes (p = 0.01). Conclusion: The genetic variants of FTO rs9939609 are associated with obesity, eating behaviour and food preferences in adolescents of Minangkabau ethnicity.
  Jamsari Jamsari , Istino Ferita , Ade Noverta , Eko Dharma Husada , Friedrich W. Herberg , Wolfgang Nellen and Lily Syukriani
  Background and Objective: In order to elucidate pathogenic determining factor of pepper yellow leaf curl virus isolated from a lowland chili pepper cultivation field in West Sumatra, Indonesia, a whole genome analysis via primer walking strategy was conducted. Materials and Methods: The whole DNA A-like genome sequence of PepYLCIpsV was elucidated via five steps of primer walking, started with universal primers PAR1c715 and PAL1v1978 as the start point. Results: Whole genome comparison analysis identified only one single base insertion/deletion event located in the common region from a previously described isolate collected from the upland location (PepYLCItdV). The BLAST comparison on the nucleotide level showed 90.6% maximal homology with the existing DNA A-like genome from many pepper yellow leaf curl viruses deposited in public database so far. Further detail comparison each of six Open Reading Frames (ORFs) between PepYLCIpsV and PpepYLCItdV indicated that V1 and V2 displayed 94-95% homology, respectively, while C2 and C3 had homology in range of 99 and 97%, respectively. Interestingly C1 and C4 showed homology only 79 and 68%, respectively and the Common Region (CR) shared only 74% similarity. Conclusion: The C1 and CR could be the determining factors for the pathogenicity, therefore, characterizing of these two regions in the population could be used for management and controlling of the virus. The pathogenic isolate PepYLCIpsV (KT809345) appears to be derived by recombination from two isolates originating from different regions and hosts. Its existence seems to be more ancient than its currently mild dominant counterpart PepYLCItdV (KT809346).
  Siti Nur Aisyah , Jefri Maldoni , Irma Sulastri , Weni Suryati , Yuli Marlisa , Lissa Herliana , Lily Syukriani , Renfiyeni Renfiyeni and Jamsari Jamsari
  Background and Objective: Assessment of the plant growth promoting (PGP) properties of a bacteria in vitro required the adjustment of appropriate culture condition. This study was aimed to optimize the culture condition required to stimulate two PGP traits (antifungal activity and indole-3-acetic acid (IAA) production) of phylloplane Serratia plymuthica strain UBCF_13. Materials and Methods: Evaluation of UBCF_13 antifungal activity against F. oxysporum f. sp. glycine was conducted under various modifications (pH and addition of exogenous carbons, Nitrogens as well as metals). This strain IAA production was optimized under various culture durations and L-tryptophan (Trp) concentrations. The resulted IAA were then inoculated to several plant’s seed to evaluate its effect on plant root and shoot growth. Data were analyzed statistically and the significance was further assessed using Duncan’s new multiple range test with a p<0.05. Results: Application of 50 μL cells-free supernatants (CFS) exhibited the highest fungal suppression under pH 5 (23.02%), (NH4)2 SO4 addition (28.25%) and CaCl2 addition (27.14%). The highest IAA concentration (116.09 μg mL1) was obtained from the 48 h culture containing 0.2% L-Trp. The IAA-containing CFS of UBCF_13 stimulated longer growth in root and shoot of Solanaceous plants (chili, tomato and eggplant). Conclusion: Antifungal activity of UBCF_13 was predicted to be dependent on acidic condition and the availability of inorganic nitrogen as well as calcium, while its IAA could be produced in short time with the low amount of Trp.
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