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Articles by Jalila Abu
Total Records ( 3 ) for Jalila Abu
  Yaqub Ahmed Geidam , Zunita Zakaria , Saleha Abdul Aziz , Siti Khairani Bejo , Jalila Abu and Sharina Omar
  Prevalence of multidrug resistant bacteria in apparently healthy chickens from 3 selected poultry farms in Selangor area of Malaysia was investigated. Conventional isolation techniques such as growth on selective media, gram staining and biochemical tests were utilised for the identification of the different bacterial isolates. Antimicrobial sensitivity test was monitored with the disc diffusion assay against 12 antimicrobial agents. A total of 96 Staphylococcus aureus, 48 E. coli, 7 Pasteurella sp. and 6 Salmonella sp. were isolated. All E. coli and Salmonella spp. isolates were multidrug resistant while 77.2% of Staphylococcus aureus and 71.5% of Pasteurella sp. isolates were multidrug resistant. The study further revealed highest resistance to tetracycline while cephalothin as the best drug of choice for treatment of infections caused by the isolates in the study area. Since not only chickens are at risk, this study recommends urgent intervention by regulatory agencies to limit the emergence and spread of these bacteria as well as prudent use of antibacterial agents among farmers in Malaysia.
  John Tang Yew Huat , Saleha Abdul Aziz , Jalila Abu , Farinazleen Mohamad Ghazali , Tuan Zainazor Tuan Chilek , Noorlis Ahmad , Afriani Sandra , Mitsuaki Nishibuchi and Son Radu
  The aim of this study was to investigate the occurrence of campylobacters in chicken at farms (close-house system and open-house system), slaughtering (conventional slaughterhouse and processing plant) and retail (wet market and supermarket). Campylobacter spp. was not found in cloacal swabs in chickens aged of 4 weeks in farms with close-house system. Campylobacter spp. was found in cloacal swabs (95.0%) in four weeks old chicken in farms with open-house systems. End-slaughtering samples from conventional slaughterhouse and processing plant were contaminated with Campylobacter spp. at 84.0% and 94.0%, respectively. Campylobacter contamination on wet market and supermarket samples with 78.0% and 92.0%, respectively. Close-house system at farm level was able to prevent or delay Campylobacter spp. colonization in chickens but contamination by Campylobacter spp. at retail level was still high. Therefore, monitoring of Campylobacter spp. in chicken products at retail level is crucial to reduce risk of human ingestion of Campylobacter spp. through chicken products.
  Richard The Swee Aun , Jalila Abu , Abdul Rahman Omar and Jayaraj Vijaya Kumaran
  This study was conducted to detect the presence of Bone Morphogenetic Protein 2 (BMP2) gene within the avian genome and cloned it into Pichia pastoris for future usage. DNA was extracted from feathers and preceded to BMP2 gene detection via Polymerase Chain Reaction (PCR). Agarose gel presented positive result for BMP2 gene amplification. Then BMP2 gene was then cloned into pCR2.1-TOPO plasmid and sent for sequencing to further confirm the presence of BMP2 gene. Followed by, inserted the BMP2 gene into Pichia Pink α-HC then allowed the BMP2 gene to express the BMP2 protein with Pichia pastoris expression System. SDS-PAGE results shown presence of the BMP2 protein and it was further confirm using Chromogenic Development method.
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