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by
Jakaria |
Total Records (
4 ) for
Jakaria |
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R.K. Rusli
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K.G. Wiryawan
,
T. Toharmat
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Jakaria
and
R. Mutia
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This research aims to study the effects of mangosteen pericarp meal (MPM) and vitamin E (VE) on the performance, blood profile, antioxidant enzyme and HSP 70 gene expression of laying hens in tropical environment when supplemented in their diets. In this study, 160 laying hens of Lohman strains were used; they were 24 weeks old and were observed for 11 weeks. They were arranged in a completely randomized design with four treatments and four replications (10 birds each). The treatments consisted of R0 (control diet), R1 (R0+1 g MPM/kg rations), R2 (R0+2 g MPM/kg rations) and R3 (R0+200 mg VE/kg rations). The data were analyzed using analysis of Variance (ANOVA); if there was any significant difference among the treatments, further tested was done using Duncan Multiple Range Test. The results showed that supplementation of MPM and VE in the diet of the hens significantly (p<0.01) increased the activity of the antioxidant enzyme superoxide dismutase (SOD) and decreased the thiobarbituric acid reactive substances (TBARS) values. Supplementing the hens’ diet with MPM and VE did not affect (p>0.05) their performance, blood profile and HSP70 gene expression. It is concluded that supplementing the diets of the laying hens with MPM and VE increased the activity of SOD and decreased TBARS values of yolk. |
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Maria Ulfah
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Dyah Perwitasari
,
Jakaria
,
Muladno
and
Achmad Farajallah
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Assessing the molecular characteristic of chickens is the powerful method to differentiate chicken breeds. This study analyses the matrilineal evolution of the Indonesian local chickens based on mitochondrial DNA D-loop region to reveal their genetic diversity and phylogenetic relationships. The estimated genetic diversity of Indonesian local chickens ranged from 0.00171 to 0.01800. Six (6) groups from 67 D-loop haplotypes of Indonesian chickens are observed in this study. The Indonesian local chickens can be differentiated from Saudi Arabian, ornamental and commercial line chickens. However, maintaining genetic variation of certain chicken population will be useful to conserve local genes for future utilization. |
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Isyana Khaerunnisa
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Muhammad Pramujo
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Irma Isnafia Arief
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Cahyo Budiman
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Asep Gunawan
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Jakaria
and
Cece Sumantri
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Myostatin, or growth and differentiation factor-8 (GDF-8), is a member of the Transforming Growth Factor (TGF)-β superfamily. This family functions as a negative regulator of skeletal muscle. Mutations in exon 2 have been reported to convert Thymine into Guanine (T4842G) that alters the amino acid leucine into arginine, which is associated with body weight in chickens. The objectives of this study were to identify the polymorphism of T4842G mutation in the myostatin gene in Indonesian chickens and evaluate their effects on carcass characteristics. The gene polymorphism was identified with the Polymerase Chain Reaction-Restriction Fragment Length Polymorphism (PCR-RFLP) method using the BsrI restriction enzyme. The effect of genotype on carcass and meat quality was analyzed using the SAS General Linear Model (GLM) procedure. Genotyping was performed on 332 chickens from 7 Indonesian chicken populations (Kampung, Merawang, Sentul, Cobb broiler, F1 crossbreed of Kampung x layer, F1 crossbreed of Kampung x Cobb broiler and F2 crossbreed of Kampung x Cobb broiler). The product of amplification was 247 bp. The myostatin|BsrI locus was polymorphic in all populations, producing two alleles (G and T) and three genotypes (GG, GT, TT). Results from the analysis of the allele and genotype frequency showed that the T allele had a higher frequency than the G allele in all populations, except for the F1 crossbreed of the Kampung x Cobb broiler chicken population, which had equal allele frequencies. A significant effect was found between genotype and carcass characteristics in the F2 crossbreed Kampung x Cobb broiler chickens. A SNP in the coding region of myostatin in exon 2 was associated with live weight, carcass weight, breast weight, thighs weight, drum sticks weight, wings weight, breast muscle weight, thighs muscle weight, drum sticks muscle weight and free water. Here, the association of myostatin|BsrI gene polymorphism with chicken carcass characteristics in Indonesian chickens has been demonstrated, providing evidence that myostatin might be an important candidate gene for chicken carcass characteristics. |
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Asep Gunawan
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Eva Siti Nurajizah
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Kasita Listyarini
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Ahmad Furqon
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Woki Bilyaro
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Cece Sumantri
,
Jakaria
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Syeda Hasina Akter
and
Muhammad Jasim Uddin
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Background and Objective: The stearoyl-CoA desaturase (SCD) gene encodes an enzyme involved in fatty acid (FA) biosynthesis. The aim of the present study was to investigate the association and expression of SCD as a candidate gene for FA composition in Indonesian crossbred chickens. Materials and Methods: Sixty-two Indonesian crossbred, unsexed chickens (F2 Kampung×broilers) were used. FA composition was measured at 12 weeks of age from thigh meat of crossbred chicken. Results: A single nucleotide polymorphism in coding region c.17492542 C>G of SCD was associated with FA composition, including both unsaturated [linoleic (C18:2n6c) and eicosadienoic (C20:2) acids] and saturated [lauric acid (C12:0)] forms. SCD mRNA expression analysis in liver revealed 6 chickens with extremely high and low FA compositions, of them, high FA birds (n = 3) had higher unsaturated and lower saturated FAs, while the low FA group (n = 3) had lower unsaturated and higher saturated FA levels. SCD expression was higher (p<0.05) in tissues collected from high FA chickens than low FA chickens. Conclusion:These results will improve the understanding of SCD function in FA composition and will shed light on SCD as a potential candidate in the selection of chickens with higher levels of unsaturated and lower levels of saturated FA. |
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