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Articles by Jain Neelam
Total Records ( 3 ) for Jain Neelam
  Oke Kingsley Oyediran , Aseri Gajendar Kumar and Jain Neelam
  Background and Objective: Arbuscular mycorrhizal fungi (AMF) symbiosis is a major survival strategy for plants growing under environmental stresses. Present study aimed at investigating diversity and drivers of AMF in rhizosphere of tomato at arid and semi-arid regions of Indian desert. Materials and Methods: Soil and feeder root samples were collected from 24 tomato farms in 6 districts of arid and semi-arid regions of the desert during 2017 cropping season. Spores were analyzed using morphological and molecular (Illumina Miseq sequencing platform) methods. Results: About 18 species viz. 8 of Glomus, 3 each of Acaulospora and Sclerocystis, 2 each of Scutellospora and Gigaspora were isolated. Glomus mossae, G. intraradices and G. fasciculatum had highest frequency of occurrence (100%) followed by Gigaspora albida, G. margarita and A. bireculata (83% each), while other species ranged between 33-66%. Spore population showed strong positive correlations with root colonization, organic carbon and rainfall, fairly positive correlation with sand, pH, nitrogen and potassium, weak correlations with temperature, silt, clay and electrical conductivity and negatively significant correlation with phosphorous. Conclusion: Glomus species were dominant AMF, spore population and root colonization were higher and lower in arid and semi-arid districts respectively, while major drivers of AMF diversity were edapho-climatic factors.
  Oke Kingsley Oyediran , Aseri Gajender Kumar and Jain Neelam
  Background and Objective: The search for sustainable means of reducing deleterious effects of chemical fertilizers in crop productivity is a global concern. Thus, the present study was carried out from 2015-2017 to investigate the potentials of in vitro developed bio-film bio-fertilizers and organic manure for reducing chemical fertilizer in okra (Abelmoschus esculentus (L.) Moench) production at Tala village (Jaipur district), Rajasthan state, India. Materials and Methods: Treatments were prepared from organic manure (OMT), arbuscular mycorrhizal fungi-Azotobacter bio-filmed bio-fertilizers (BFT), bio-filmed bio-fertilizers+organic manure (OBT), 65% recommended rate chemical fertilizer (CHT) and an untreated (UT) control in three replicates on randomized block design layout. Results: The cumulative effect of OBT treatment produced the highest values for all the parameters examined. Followed by the BFT and OMT treatments which were similar. The CHT treatment was next and the untreated control (UT) treatment had the lowest values for all parameters analyzed. Conclusion: There is the possibility to customize combinations of bio-filmed bio-fertilizers and organic manure for optimizing economics of production by reducing chemical fertilizer application.
  Oke Kingsley Oyediran , Aseri Gajender Kumar and Jain Neelam
  Background and Objective: Efficacy of rhizosphere microorganisms to synthesis phytohormone is a key survival strategy for plants growing under environmentally stressed conditions. Thus, this study aimed at investigating potentials of four indigenous rhizobacteria of vegetables at semi-arid agro-zone of Rajasthan desert, India, to synthesize Indole Acetic Acid (IAA), cytokinin (CK) and Gibberellic Acids (GA3). Materials and Methods: Isolates (Azospirillum brasiliense (IR25), Azotobacter chrocoocum (IR28), Pseudomonas stutzeri (IP32) and Pseudomonas putida (IP35)) were screened for plant growth-promoting attributes. HPLC, TLC and spectrophotometry were used for phytohormone characterization, recovery and quantification at 72, 96 and 120 h time intervals. Results: All isolates possessed substantive growth-promoting traits and produced pink, fluorescent and green colors confirming synthesis of IAA, CK and GA3, respectively. At 96 h time interval, highest IAA was recorded by IR25 followed by IP35 which was slightly higher than IR28 and IP32. Maximum CK was found in IR25 followed by IR28 while IP35 recorded slightly higher concentration than IP32. GA3 recorded by IR25 was highest, IP35 was next with higher value than IR28 and IP32. Conclusion: All isolates produced significantly high phytohormones that can be transformed into effective bio-formulations for use in sustainable vegetable production at semi-arid agro-zone of Rajasthan desert.
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