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Articles by Jaime A. Teixeira da Silva
Total Records ( 3 ) for Jaime A. Teixeira da Silva
  Jaime A. Teixeira da Silva , D.T.T. Giang and M. Tanaka
  To overcome various disadvantages of conventional culture vessels and to employ photoautotrophic micropropagation techniques and in order to reduce the cost of film culture vessels, a multiple-layered and very inexpensive OTP® film made of TPX (4-methyl-1-pentane polymer) and CPP (a polypropylene) with characteristics similar to the PFA (tetrafluoroethylene perfluoroakyl vinyl ether copolymer) film, has been used to make a novel disposable film culture vessel, termed the Vitron. In this study, Epidendrum (E. Rouge Magic x E. Joseph Lii Mother’s Day Koto) shoots at the three leaf stage were cultured under high CO2 enrichment at a low photosynthetic photon flux density in three film culture systems: Miracle Pack (MP) using PFA film, MP using OTP film and Vitron with sugar-free liquid modified Vacin and Went medium and rockwool multiblockTM as a substrate. The in vitro and ex vitro growth of Epidendrum plantlets cultured in the three film culture systems were significantly similar, producing normal and vigorous plantlets. The net photosynthetic rate of in vitro Epidendrum plantlets cultured in the three film culture systems were also significantly similar, suggesting that the novel Vitron film culture system can replace conventional culture vessels for Epidendrum photoautotrophic micropropagation. This study further emphasizes that Epidendrum plantlets can be well-rooted and acclimatized in vitro and transfer to a greenhouse does not require any additional acclimatization step, which is usually an additional economic and time-consuming step.
  Jaime A. Teixeira da Silva , Dam D.T. Giang and Michio Tanaka
  In order to reduce the time, cost and energy required to acclimatize Cymbidium and Musa in in vitro micropropagated plants, growth under 2 culture systems: Miracle Pack® with a) PFA film or b) OTP film were compared. The substrate was either rockwool or agar. In all cases 3000 ppm CO2 enrichment 24 h/day at a low PPFD (45 μ mol m-2 s-1) with sugar-free liquid medium was provided, i.e. photoautotrophic micropropagation. Either culture system was found to be suitable for micropropagation with higher fresh and dry shoot, root and total weights, with enhanced photosynthetic capacity (higher SPAD (measure of chlorophyll content) and photosynthetic rates).
  N.A. Siddique , Jaime A. Teixeira da Silva and M.A. Bari
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