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Articles by Jaime A. Teixeira da Silva
Total Records ( 4 ) for Jaime A. Teixeira da Silva
  Jaime A. Teixeira da Silva
  This study provides detailed reproducible methods for the transformation of Dendranthema X grandiflora Shuhou-no-chikara (standard) and Lineker (spray) stem internode Thin Cell Layers (TCLs) and conventional stem internode explants, using intron-containing and intron-less plasmids. Discussed methodologies and results have used GUS reporter, nptII selector genes, as well as pac1/2,5-A/RNaseL genes coding for virus/viroid resistance. Transformation efficiencies are reported at the kanamycin selection (callus formation and plantlet rooting), GUS, PCR, Southern and Western levels. Notes on regeneration improvement through the use of TCLs, as well as the effective use of sonication for both regeneration and generation of transformants, stimulation of Agroinfection and elimination of Agrobacterium, are included. Protocols are provided for the use of scanning electron microscopy to confirm developmental processes, as well as flow cytometry to check for cell competence and division stages. The difficulties still experienced in the genetic transformation of chrysanthemum globally by researchers require a detailed protocol that exposes the subtleties in methodology. This study provides that information source.
  Jaime A. Teixeira da Silva
  Cut flowers are the big revenue creators of fresh commodities. In this review, the current status of postharvest technology applied to cut flowers and foliage is discussed. Also included are considerations of the physical, biochemical and genetic mechanisms underlying some of the processes central to cut flower and foliage deterioration, such as abscission, senescence and programmed cell death. Moreover, through examples, solutions to increasing longevity through improvement of cultural practices and sanitation and through genetic engineering are covered, providing practical solutions to the global cut flower market.
  N.A. Siddique , Jaime A. Teixeira da Silva and M.A. Bari
  Folk medicines are complemented with other systems of traditional medicines. An account of about 200 hundred plants used in medicine among certain herbal doctors of Bangladesh is given; details of local name, scientific name, method of application or administration and dosage are given. These records include several uses which do not seem to be known or recorded in literature. It is discussed that indigenous knowledge assists in the wider application and acceptance of traditional medicine. This study makes it amply clear that the wild medicinal plants in Bangladesh folk life are used to meet all kinds of requirements. Our grandfathers and grandmothers are the carrier of indigenous knowledge regarding medicinal plants but today we are not conserving this knowledge due to decrease indigenous people. So it is now important to preserve this indigenous knowledge.
  N.A. Siddique , Jaime A. Teixeira da Silva , M. Anisuzzaman and M.A. Bari
  A procedure for rapid in vitro propagation of the aromatic and medicinal plants Aristolochia indica Linn and Hemidesmus indicus R.B (Family: Aristolochiaceae and Asclepiadaceae respectively) from axillary shoots is described. The highest percentage of callus induction was 92% on MS medium supplemented with 1.0 mg L-1 α-naphthalene acetic acid (NAA) and 1.0 mg L-1 6-benzyladenine (BA) for A. indica while 98% was achieved on MS medium supplemented with 0.5 mg L-1 NAA and 2.0 mg L-1 Kinetin (Kn) for H. indicus. The colour of the calli was mostly light to dark green. Development of adventitious shoots occurred when calli were subcultured on MS medium supplemented with BA and Kn alone or in BA combined with NAA and IAA, or NAA, IAA and BAP combined with Kn. The highest percentage (90%) of shoot regeneration in A. indica was obtained on MS medium fortified with 1.0 mg L-1 BA and 2.5 mg L-1 NAA, but a 98% rate on MS medium supplemented with 2.5 mg L-1 Kn and 1.0 mg L-1 NAA for H. indicus. Regenerated shoots rooted best on MS medium containing 2.5 mg L-1 Kn and 2.0 mg L-1 IBA. Plantlets were transferred to pots containing sand and soil mixture and acclimatized in a culture room. Finally rooted plants were transferred to soil.
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