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Articles by J.W. Tachie-Menson
Total Records ( 2 ) for J.W. Tachie-Menson
  J.N. Buah , J.W. Tachie-Menson , G. Addae and P. Asare
  The suitability of sugarcane juice for the in vitro culture of bananas and plantains was investigated in this study. Two concentrations of sugar cane juice 5, 10% and 30 g L-1 laboratory sucrose were used. There was also a control medium without carbon source. Explants were prepared from field grown sword suckers. The preparation of the explants involved, sterilization with 70% ethanol, followed by 1% Sodium hypochlorite+1% tween 20. The explants were cultured at 26°C, under 16 h photoperiod, light intensity of 3000 lux and a relative humidity of 60%. Plants that were cultured on 5% sugarcane juice had higher average fresh weight values of 16.6 g per plant compared to those that were cultured on the 30 g L-1 sucrose and 10% sugarcane juice which had fresh weight of 15.6 g per plant and 11.0 g per plant, respectively. Oniba had higher fresh weight on medium supplemented with 5% sugarcane juice compared to the two other cultivars. In terms of dry weight, ‘Oniaba’ again had the highest value of 7.2 g per plant which was obtained on medium supplemented with 5% sugarcane juice. Plants that were cultured on 5% sugarcane juice were better in terms of shoot height and number of shoots per plant than those cultured on 30 g L-1 sucrose and the differences between the 5% sugarcane juice and the other treatments were statistically different. The various carbon sources and their concentrations also affected the water potential of the media. Medium supplemented with 10% sugarcane juice had the lowest water potential of -0.8 MPa compared to the control medium that had a water potential of -0.1 MPa. Medium supplemented with 5% sugarcane juice and 30 g L-1 sucrose had in a medium water potential of -0.3 MPa. Five percent sugarcane juice was found to be a better substitute for laboratory grade sucrose for the in vitro propagation of Musa sp.
  J.N. Buah and J.W. Tachie-Menson
  The suitability of bud manipulation technique as an alternative to tissue culture in the regeneration of healthy planting material for farmers was investigated from May 2013 to August 2013, at the University of Cape Coast. The bud manipulation technique was carried out where the apical meristem of the corm was cut through to remove apical dominance effect. One hundred and twenty corms from sword suckers were used for each of the techniques, forty for each of the three cultivars (2 plantain cultivars and 1 banana cultivar). The tissue culture protocol was used for the in vitro culture. Generally, the explants produced more shoots in vitro compared to the bud manipulation technique. The highest number of shoot, 23 per explant under the in vitro technique was from the plantain cultivar Apantu whilst 15 shoots per explant were produced from the same cultivar, with the bud manipulation technique. The same trend was observed with the shoot height. Tissue cultured shoots had a maximum mean shoot height of 25 cm from Apantu whilst bud manipulation technique produced plantlets with maximum mean shoot height of 14 cm. The three cultivars performed better with tissue culture and of the three cultivars, Apantu had a better performance. Even though tissue culture produced more shoots compared to the bud manipulation technique, the number of shoots, shoot height and percentage survival of plantlets from the bud manipulation makes the bud manipulation technique a suitable alternative to tissue culture since it is farmer friendly and less expensive.
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