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Articles by J.T. Shu
Total Records ( 3 ) for J.T. Shu
  W.B. Bao , J.T. Shu , H.H. Musa and G.H. Chen
  Genetic distance and gene flow of all pairwises of 14 Chinese indigenous chicken breeds and one jungle fowl and their relations with geographical distances were evaluated in the present study. The pairwise FST/(1-FST) of 15 Chinese chicken breeds was evaluated by 29 microsatellite loci. The results showed that the number of alleles per locus was ranged from 2-25 and the average of expected heterozygosity and PIC of all loci were 0.6683 and 0.50, respectively. The average of genetic differentiation among population measured as FST value, was 16.4% (p< 0.001), all loci were contributed significantly (p< 0.001) to this differentiation. Red jungle fowl and Gushi chickens were observed distant genetic relationship with other breeds, whereas Huainan Partridge and Tibetan chickens were observed close relationship with other breeds. The geographical elements may own the close relationship for particular population pairs. However, the equation FST/ (1-FST) = -0.0162 + 0.0313ln (d) and the result of Mantelís test (p = 0.054) did not provide enough support for a significant correlation between the genetic and geographical pairwise distances.
  H.H. Musa , J.H. Cheng , W.B. Bao , J.T. Shu , B.C. Li and G.H. Chen
  Tissues specimens include (liver, kidney, small intestine and abdominal fat) were excised from lean (Rugao) and fat (Anka) chickens, both breeds were raised under the same conditions. The expression of LDL receptor mRNA in various tissues of genetically fat and lean chickens were determined by semi-quantitative RT-PCR. The level of gene expression was determined as the ratio of integrated peak area for each individual gene PCR product relative to that of the co-amplified ß-actin internal standard. The results shows that the expression of low density lipoprotein receptor mRNA was significantly (p< 0.05) different between abdominal fat and liver tissues, while they were non significantly different between the other tissues. The levels of LDLR1 mRNA expression in intestine, kidney and abdominal fat tissues were none significantly different between genetically fat and lean, while it was significantly (p< 0.05) different in liver. In addition, the levels of LDLR5 mRNA expression in various tissues were non significantly different between genetically fat and lean chickens.
  H.H. Musa , G.H. Chen , K.H. Wang , B.C. Li , D.M. Mekki , J.T. Shu and H.P. Ju
  Serum cholesterol, lipoprotein concentration and carcass characteristics data were taken in 30 fat and 40 lean chickens. Breeds were differed significantly (p<0.001) in carcass traits and non significantly (p>0.05) in carcass portions. Color density and pH were also differed significantly (p<0.05), whereas water holding capacity (WHC) and shear force value were non significant (p>0.05). Both breeds showed positive correlation for carcass traits, while abdominal fat was negatively related with breast muscle in lean breed. Significant difference (p<0.05) were observed in cholesterol and LDL and non significant (p>0.05) in triglyceride, VLDL and HDL level. Cholesterol and triglyceride were respectively related with LDL and VLDL. HDL was observed negatively related with triglyceride and cholesterol. Sex was effect significantly (p<0.05) in triglycerides and VLDL level and non significantly (p>0.05) in cholesterol, HDL and LDL level in fat breed. However, lean breed observed non significant difference (p>0.05) for lipoprotein concentrations. Correlation of lipoprotein concentrations and carcass trait were determined. In both breeds lipoprotein concentrations were negatively related with carcass traits. LDL was positively related with breast muscle and abdominal fat weight and triglyceride with live weight in fat breed. In lean breed cholesterol and LDL were presented positive correlation with all carcass traits.
 
 
 
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