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Articles by J.S. Ruwaan
Total Records ( 2 ) for J.S. Ruwaan
  B.O. Omontese , P.I. Rekwot , H.J. Makun , J.A. Obidi , J.S. Ruwaan and N.P. Chiezey
  The efficiency of EAZI-Breed™ CIDR® and FGA-30® intravaginal sponges in synchronizing estrus was investigated in prepartum Yankasa Ewes. About 20 randomly cycling pre-partum Yankasa ewes aged between 1.5-2 years and weighing between 13-15 kg was used for this study. They were randomly assigned into two groups, Group A (FGA, n = 10) and Group B (CIDR®, n = 10) for 14 days. Natural mating by a fertile ram was performed following progestagen withdrawal for ewes detected to be on heat. Estrus response in Group A and B was 70 and 80%, respectively. The time to estrus onset following progestagen withdrawal for FGA-30 and CIDR (Mean±SEM) was 43.60±6.98 and 23.57±4.07 h, respectively. In Groups A and B, the duration of induced estrus was (46.65±3.08 and 53.90±5.87 h) while estrus cessation was (90.37±8.44 and 77.92±4.24 h) post withdrawal of the devices. The interval from withdrawal of progestagen to onset of estrus was (p<0.05) longer in FGA than in CIDR (43.60±6.98 vs. 23.57±4.07 h). However, the duration of induced estrus period was shorter in the FGA group than the CIDR group. Retention rate was lower in group A (60%) than B (90%). Drawstring breakage observed in FGA sponges was absent in CIDR devices (20% versus 0) while vaginal discharge rate was higher in group A. These results show that although FGA and CIDR devices are equally efficient in synchronizing estrus in prepartum Yankasa ewes, CIDR provides higher estrus response rate, shorter time to estrus, longer duration of estrus, higher retention rate and ease of application. Consequently, the use of CIDR is recommended.
  J.S. Ruwaan , P.I. Rekwot , P.A. Abdu , B.O. Omontese , J.A. Obidi and N.P. Chiezey
  About fifty 20 weeks old Shika Brown (SB) cocks were used in this study. Five cocks consisting of three Red Shika-Brown (RSB) and two White Shika-Brown (WSB) were bled for serum samples for testosterone assay at weeks 1, 3 and 6 pre and post-infection with a Velogenic Newcastle disease virus. Blood samples were collected at 30 min interval for 3 h from each cock on the days of sampling. The blood samples were centrifuged in a Hermle Z364 centrifuge at 251.6x g for 15 min with the sera obtained stored in serum vials and kept in a deep freezer at -20°C until analysis using the Enzyme Linked Immunosorbent Assay (ELISA) technique. At the end of the study, twenty control (n = 20) and twenty infected (n = 20) cocks were slaughtered. Their testicles were removed, measured, minced and ground for the determination of gonadal sperm reserves. The mean testosterone concentration of both the control SB cocks and the pre-infected SB cocks had no particular pattern. The mean testosterone concentration post infection showed a decrease from week 1-6. The mean testosterone concentration peak for the control red SB cocks was 1, 2 and 2.5 nmols mL-1 at weeks 1, 3 and 6, respectively while the white SB cocks had 12.5, 5.5, 3 nmols mL-1 at weeks 1, 3 and 6, respectively. The infected red SB cocks had mean testosterone concentration peaks of 9.7, 6.3 and 2.7 nmols mL-1 at weeks 1, 3 and 6 post-infection, respectively while the white SB cocks had a mean testosterone concentration peak of 6.5, 14.5 and 6.5 nmols mL-1 at weeks 1, 3 and 6 post-infection, respectively. The gonadal sperm reserves of the control red and white SB cocks were not significantly different but the gonadal sperm reserves of the control white SB cocks was significantly (p>0.05) higher than the gonadal sperm reserves of the infected red and white SB cocks. The total gonadal sperm reserve of the control white cocks was significantly (p>0.05) higher than the total gonadal sperm reserves of the infected red and white SB cocks.
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