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Articles by J.O. Orji
Total Records ( 2 ) for J.O. Orji
  I.B. Moses , C.O. Esimone , I.R. Iroha , E.N. Ugbo , A.C. Nwuzo , J.O. Orji , E.A. Nwakaeze , N.B. Agumah , A.A. Emioye , E.G. Ukpai and L.N. Ogene
  Background and Objective: The increase in antibiotic-resistant Staphylococcus pseudintermedius among pets and the transfer of S. pseudintermedius from pets to humans threaten veterinary medicine and public health. The objective of this study was to determine the antibiotic resistance patterns and prevalence of virulence genes among Staphylococcus pseudintermedius isolates obtained from dogs and dog owners in Abakaliki, Nigeria. Materials and Methods: Swab samples from 112 shelter dogs and 97 dog owners were processed using standard microbiological procedures. Antibiotic susceptibility test was done by Kirby Bauer disc diffusion technique. Screening for virulence genes was done by Polymerase Chain Reaction (PCR). Results: Exactly 99 S. pseudintermedius isolates including 52 Methicillin Resistant Staphylococcus pseudintermedius (MRSP) strains were recovered from dogs and humans. Sec, exi, siet and lukD virulence genes were confirmed by PCR. Isolates were highly resistant (69.2-100%) to amoxicillin, cefepime and ceftazidime, but very susceptible (66.3-80.2%) to ofloxacin, doripenem, ertapenem and chloramphenicol. A total of 73 (73.7%), 2 (2%), 62 (62.6%) and 55 (55.6%) isolates harboured sec, exi, siet and lukD virulence genes, respectively. Conclusion: Isolates in this study harboured sec, siet, exi and lukD virulence genes and were also notably more resistant than those reported in literature. There was phenotypic homogeneity in the antibiotic resistance profiles of isolates from both humans and dogs in households that were sampled, thus depicting a possible zoonotic transmission event from dogs to their owners. Hence, monitoring of antimicrobial resistance, appropriate hygienic measures such as; hand washing after attending to dogs should be adopted so as to minimize the spread of resistance and virulence.
  I.R. Iroha , E.S Amadi , J.O. Orji , A.C. Ogabus , A.E. Oji and C.O. Esimone
  The present study evaluated the antimicrobial effect of different combinations of gentamicin and floroquinolones (ciprofloxacin, ofloxacin, perfloxacin and gatifloxacin) against extended spectrum beta lactamase (ESBL) positive E. coli isolates using checkerboard method. One hundred and four clinical isolates of E. coli obtained from urine (26), blood (24), stool (20), sputum (19) and semen (14) were investigated for ESBL production. Fifteen (14.4%) were positive for ESBL production in the following order: 4(3.8%) from urine, 3(2.8%) from blood, 3(2.8%) from stool, 3(2.8%) from sputum and 2(1.9%) from semen. Five representative ESBL positive isolates, one from each specimen, were investigated for their susceptibility patterns to different gentamicin and floroquinolone combinations. The combinations of gentamicin and ciprofloxacin, perfloxacin and gatifloxacin, respectively at different ratios were predominantly synergistic in activity while gentamicin and ofloxacin combinations were primarily indifference in activity. These results may have some therapeutic significance in the management of ESBL infections especially in areas of the world where ESBL organisms are either emerging or re-emerging.
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