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Articles by J.O. Ajele
Total Records ( 4 ) for J.O. Ajele
  A. Onasanya , M.M. Ekperigin , Y. Sere , F.E. Nwilene , J.O. Ajele and G. Oboh
  Isozyme fingerprinting and differentiation of Xanthomonas oryzae pv. oryzae (Xoo) isolates, causing rice Bacterial Leaf Blight (BLB) disease in West Africa, was carried out. Of 13 enzyme systems screened, Glucose 6-phosphate dehydrogenase (G6PH) showed adequate resolution and enzyme activity. Thus total proteins from all the 30 isolates were then analyzed using G6PH. This enzyme system was potentially useful as they differentiate all the 30 Xoo isolates studied. The study revealed 40-96.7% polymorphism in G6PH loci within the Xoo enzyme profile. These polymorphic isozyme loci were used to construct phylogenetic relationship cluster dendrogram among the 30 Xoo isolates. All the 30 Xoo isolates were classified into two major genetic groups (Xoo-A and Xoo-B) with five subgroups. Xoo-A possibly covers 46% and Xoo-B 54% of BLB population across West Africa. This study suggests the emergence of subgroup genotypes possibly the result of mutations and interactions among isolates and strains that originally made up Xoo-A and Xoo-B genotypes. The isozyme fingerprint defined for each race of Xoo could be useful for epidemiological surveys, disease diagnoses and in the identification of new virulent strains, isolates and their origin. This information could be useful in rice breeding programs aiming at development of durable Xoo resistant rice cultivars to different rice ecologies and localities in West Africa.
  B.O. Akanji , J.O. Ajele , A. Onasanya and O. Oyelakin
  Genetic fingerprinting of 30 Pseudomonas aeruginosa (Pa) isolates from three types of nosocomial infection cases from two Osun State Teaching Hospitals was compared using RAPD-PCR markers. Ten out of 50 operon primers tested showed polymorphism with reproducible results among the isolates and produced 131 bands of which 74 were polymorphic with sizes ranging between 200 and 3,000 bp. Cluster analysis using the 74 polymorphic markers classified the 30 Pseudomonas aeruginosa isolates into two (PgA and PgB) genetic groups. Comparing isolates proportion in each genotype based on their site of infection, antibiotics resistance pattern and geographical location, it was revealed that the proportion of urinary tract infection isolates in PgA genotype was significantly less than those in PgB genotype (z = -1.195, p<0.05) while the proportion of septicaemia isolates in PgA genotype was significantly higher than its proportion in PgB genotype (z = 1.348, p<0.05). However the proportion of wound infection isolates of PgA and PgB genotypes were significantly the same (z = -0.278, p>0.05). The PgA genotype contained few isolates with increased virulence and resistance to new antimicrobial modules and could possibly be new emerging P. aeruginosa strains from PgB genotype population. The study has critically revealed the genetic diversity and distribution among P. aeruginosa isolates in Osun State.
  A. Onasanya , M.M. Ekperigin , Y. Sere and J.O. Ajele
  Genetic diversityof 30 Xanthomonas oryzae pv. oryzae (Xoo) isolates, causing rice bacterial leaf blight disease in West Africa, was carried out using isozyme PAGE analysis. Of 13 enzyme systems evaluated, SKDH, EST and G6PH showed adequate resolution, enzyme activity and polymorphism and were used to analyze the total proteins from all the 30 isolates. The study revealed 23 isozyme loci in which SKDH produced 33.3-93.3% polymorphism, EST and G6PH equally gave 40-96.7% polymorphism within the Xoo isolates enzyme profile. These 23 isozyme loci were used to construct phylogenetic relationship cluster among 30 Xoo isolates, of which the Xoo isolates were classified into two major genetic groups (Xoo-A and Xoo-B) with two subgroups each (Xoo-A1 and Xoo-A2) and (Xoo-B1 and Xoo-B2). The 23 isozyme markers obtained clustered into 3 major groups (Gp-1, Gp-2 and Gp-3). Genetic study revealed that Gp-1 is genetically linked to the identification of Xoo-A1 genotype, Gp-2 to Xoo-A2 and Gp-3 characterized Xoo-B1 and Xoo-B2 genotypes. The distinct pattern of each isolate obtained suggests high level of genetic variation and frequent occurrence of mutants in Xoo isolates in different host cells. This information could be useful in rice breeding programs aiming at development of durable Xoo resistant rice cultivars to different rice ecologies and localities in West Africa.
  O.O. Ojo and J.O. Ajele
  Beta-amylase is a starch hydrolyzing enzyme which is fondly used in both foods, pharmaceutical and brewing industries to convert starch into maltose. Hence, this study was carried out to isolate, purify, characterize and determine the possible involvement of histidine and cysteine in the catalytic mechanism of beta-amylase sourced from cassava (Manihot esculenta, Crantz) peels. Beta Amylase was obtained from cassava peels and purified by gel filtration and ion exchange chromatography. The homogeneity of the enzyme was established by polyacrylamide gel electrophoresis and its molecular weight by Sodium Dodecyl Sulphate Polyacrylamide Electrophoresis (SDS-PAGE) on 10% gel. The Michealis-Menten constant, Km, maximum velocity, Vmax and Kcat were obtained from Line-Weaver Bulk plot. From the plot of logVmax/km Vs pH, the apparent pk values of 5.21 and 6.58 were obtained. Effects of temperature, pH, salts concentration and temperature on stability of beta amylase activity at pH ranging from 5-8 were determined. The polyacrylamide gel electrophoresis in the presence and absence of SDS produced a single bond. The enzyme was found to have an optimum activity at pH 5 and 60oC. The current work confirmed the presence of beta-amylase in cassava peels and was also found to be thermostable and thermoactive, good enough for some industrial applications.
 
 
 
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