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Articles by J.M. Panandam
Total Records ( 7 ) for J.M. Panandam
  A.Q. Sazili , M.Z. Noor Azihan , M. Shuhaimi , M. Hilmi and J.M. Panandam
  The present study was conducted in an attempt to study the effects of the type, pH and molarity of extraction buffer on protein extractability from beef Longissimus dorsi (LD), Supraspinatus (SS) and Semitendinosus (ST) muscles. All muscle samples were dissected out from carcass immediately after slaughter and subjected to extractions using freshly prepared buffers of different buffer type (Tris Base and Tris HCl), pH (8.3 and 7.5) and molarity (100 and 20 mM). Following extraction, the total extractable protein concentration was determined by Bradford assay. The results exhibited significant (p<0.05) effects of muscle type X buffer interaction on extracted total protein concentration. The statistical analysis also revealed interaction between type of buffer, pH and molarity significantly (p<0.01), affected the extracted protein concentration. Based on the results, optimal buffer suggested to use for muscle protein extraction is Tris-Base with pH 8.3 and 100 mM. The present study demonstrated that the extractability of skeletal muscle protein was significantly influenced by the type, molarities and pH of the extraction buffers used.
  T.K. Leo , D.E. Leslie , S.S. Loo , M. Ebrahimi , Z.A. Aghwan , J.M. Panandam , A.R. Alimon , S.A. Karsani and A.Q. Sazili
  The objective of this study was to evaluate the differences in growth performance and carcass characteristics of Bali cattle subjected to oil palm integration and two different feedlot finishing systems (basal vs. high energy). Eighteen, 24-30 months old male Bali cattle were involved in this study. The animals were randomly allotted into 3 feeding groups: Integration (INT), (n = 6 animals), Feedlot A (FA) with basal energy (n = 6 animals) and Feedlot B (FB) with high energy (n = 6 animals). The animals assigned to the integration system were allowed to graze on the native forages and legumes available under the oil palm plantation. The basal energy diet consisted of 5 kg Palm Kernel Cake (PKC) pellets + ad libitum corn stover and the high energy diet which consisted of 5 kg Palm Kernel Cake (PKC) pellets + ad libitum corn stover + 400 g calcium soap of palm oil fatty acids (Megalac®, Volac International Limited, UK) were fed to the animals in FA and FB, respectively. The feeding trial was conducted for 120 consecutive days excluding 3 weeks of adaptation period. The present data suggest that some of the growth parameters and carcass traits in Bali cattle can be enhanced through the feedlot finishing system.
  D.E. Leslie , T.K. Leo , S.S. Loo , Z.A. Aghwan , A.R. Alimon , J.M. Panandam , S.A. Karsani and A.Q. Sazili
  The study was carried out in an attempt to assess meat quality of Bali cattle finished in 3 different systems. Twenty one bulls were selected from an existing herd under an oil palm plantation and randomly assigned to 120 days of feeding in; Integration-INT (n = 8), basal energy Feedlot-F (n = 6) and high energy Feedlot-FB (n = 7) System. All animals were humanely slaughtered at a commercial abattoir. Samples of Longissimus Dorsi (LD), Supra Spinatus (SS) and Semi Membranosus (SM) muscles were collected and prepared accordingly for the determinations of pH, cooking loss, shear force and color (L* and a*) values. The animals finished on Integration (INT) demonstrated higher L* values (p<0.05) in SS muscle and lower L* values (p<0.05) in both LD and SM muscles. Lower cooking losses (p<0.05) were observed in LD and SM muscles from the INT group. However, there was no difference in pH, a* (redness) and shear force values among the treatments and these were consistently shown in all three muscles. The results from this study demonstrate the influence of finishing system on Bali cattle meat quality.
  D. Aziz , S.S. Siraj , S.K. Daud , J.M. Panandam and M.F. Othman
  Information on the genetic diversity of Penaeus monodon throughout its natural range in Malaysia is still limited even though it is a highly exploited species, thus this study was undertaken to genetically characterize the prawn populations. The P. monodon samples were randomly collected from Malaysian waters and were characterized using thirty polymorphic primer pairs which showed high level of polymorphism. The total number of alleles per locus ranged from 3 to 36 with allele size ranging from 100 to 275 base pairs. The mean observed heterozygosity (0.5166) was less than the expected (0.5552), highly significant deficiencies in heterozygotes were detected in total inbreeding (FIS = 0.5500) and pair-wise genetic differentiation (FST = 0.6308) among the populations. Both the (χ2) chi-square and (G2) likelihood ratio tests detected significant differences (p<0.05) which showed a deviation from the Hardy-Weinberg equilibrium, indicating a probable inbreeding might have occurred in the populations. A Cluster analysis based on genetic distance revealed a fair genetic relationship among all the populations and the pattern was in accordance to the populations’ geographical origins. The highest genetic distance (0.7588) was observed between Lawas and Pulau Sayak populations while the lowest genetic distance (0.1191) was recorded between the Endau Rompin and Sedili populations. Various levels of genetic diversity of the P. monodon reported in this study indicated their genetic status in Malaysian waters and suitability for breeding and culture purposes. This information provides a basis for improvement through selective breeding and in the design of suitable management guidelines for this genetic material.
  B. P. Keong , S.S. Siraj , S.K. Daud , J.M. Panandam and S.A. Harmin
  Inheritance of background body colour in molly, Poecilia is not well documented despite being an economically important aquarium fish. This study was attempted to understand the inheritance of background body colour between crosses of Poecilia latipinna (non-black) and Poecilia sphenops (black) through controlled breeding. A total of 13 breeding crosses which consist of two pure bred crosses, six parental hybrid crosses, two backcrosses, two test crosses and one full-sib cross were successfully initiated. In molly, Poecilia, non-black body colour is completely dominant over black and is not sex-linked. Multiple genes interaction which acted nonadditively was also found to be influencing this phenotypic trait. However, such interaction effect was restricted in crosses generated between genetically related fish (backcross and F2 full-sib) and was absent in crosses between distantly related fish (testcross). Perhaps, such occurrence was a natural mechanism to maintain the vibrancy of non-black colouration.
  M. Ramin , A.R. Alimon , J.M. Panandam , K. Sijam , A. Javanmard and N. Abdullah
  The digestion and Volatile Fatty Acid (VFA) production from rice straw and oil palm fronds by cellulolytic bacteria isolated from the termite Coptotermes curvignathus were investigated. The bacteria were Acinetobacter strain Raminalimon, Enterobacter aerogenes strain Razmin C, Enterobacter cloacae strain Razmin B, Bacillus cereus strain Razmin A and Chryseobacterium kwangyangense strain Cb. Acinetobacter strain Raminalimon is an aerobic bacterium, while the other species are facultative anaerobes. There were significant differences (p<0.05) among the bacteria for Dry Matter (DM) lost and acetic acid production from rice straw and Acinetobacter strain Raminalimon showed the highest activity. The facultative bacteria C. kwangyangense strain Cb (cfu mL-1 231x10-6, OD: 0.5), E. cloacae (cfu mL-1 68x10-7, OD: 0.5) and E. aerogenes (cfu mL-1 33x10-7, OD: 0.5) were used for digestion study with the rumen fluid microflora. The in vitro gas production technique was applied for the comparative study and the parameters measured were pH, gas (volume), dry matter lost, acetic acid, propionic acid and butyric acid concentrations. pH was not significantly (p<0.05) different among the five treatments. The bacterium C. kwangyangense strain Cb showed the highest activity (p<0.05) for DM lost, acetic acid, propionic acid and butyric acid production from rice straw when compared to the other bacterial activities. There was no significance (p<0.05) difference between the three bacteria for the dry matter lost of oil palm fronds but the production of Volatile Fatty Acids (VFA) was significantly (p<0.05) high in the treatment which was inoculated with C. kwangyangense strain Cb. The Gen Bank NCBI/EMBL accession numbers for the bacterial strains are EU332791, EU305608, EU305609, EU294508 and EU169201.
  M. Ramin , A.R. Alimon , N. Abdullah , J.M. Panandam and K. Sijam
  In this study the lower termite Coptotermes curvignathus (Holmgren) and higher termite Macrotermes gilvus (Hagen) were identified from different parts in the vicinity of University Putra Malaysia (UPM). We isolated three enteric bacteria from the hindgut of Coptotermes curvignathus (Holmgren). All isolates were facultative anaerobes. The isolates were identified as Enterobacter aerogenes, Enterobacter cloacae and Clavibacter agropyri (Corynebacterium) by using BIOLOG assay and Bergey’s manual. The bacteria were able to assimilate carboxymethylcellulose (CMC) and cellobiose.

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