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Articles by J.M. Kataria
Total Records ( 2 ) for J.M. Kataria
  A.S. Yadav , G.K. Saxena , V.K. Saxena and J.M. Kataria
  Salmonella is an important food-borne pathogen associated with poultry. Prevalence of Salmonella was assessed in fecal swab samples collected from broiler birds of native farms in which 5% samples were found positive by conventional and PCR based methods. Out of 200 fecal swab samples 10 samples were Salmonella. Out of these Salmonella isolates, 4 were serotyped as Salmonella Typhimurium, 5 as untypable and 1 as rough strain. Survival of Salmonella Typhimurium and Salmonella Enteritidis was assessed at 30, 42 and 50°C for 24 h under laboratory conditions and results revealed that both serotypes survived at 50°C up to 24 h. Expression profiling of genes conferring survival and thermotolerance to these serotypes was assessed at high temperature using real-time PCR with transcripted RNA, after exposure at 42 and 50°C for 10 and 20 min in a water bath. Transcriptional profiling analyzed to study the relative expression of thermotolerance genes (rpoE, rpoH, rpoS, htrA, uspA and uspB) showed over expression of rpoE, rpoH and htrA genes at 5°C in both serotypes. Since S. Typhimurium exhibited higher expression of various genes when exposed to 50°C for 20 min, it may have better ability to respond to higher temperatures stress compared to Salmonella Enteritidis. Both serovars showed higher attachment to skin after exposure to temperature, which may lead to cross-contamination and foodborne illness. This study will be helpful for the poultry processors to design new intervention strategies for the effective destruction of such type of Salmonella cells on dressed poultry carcasses.
  K.A. Naveen , S.D. Singh , J.M. Kataria , R. Barathidasan and K. Dhama
  Newcastle Disease (ND) is a highly contagious infection of poultry which manifest in a wide range of severity from subclinical infection to lethal disease. In the past, a number of Newcastle disease outbreaks in poultry and other bird species have been ascribed to pigeon paramyxovirus type-1 (PPMV-1) infection. The conventional in vivo pathogenicity tests to assess the pathogenicity of PPMV-1 viruses have provided equivocal results. Lately, restriction enzyme analysis technique has been employed for unequivocal identification of individual strains of Newcastle Disease Virus (NDV) in poultry. In this study, sequence analysis of the F1/F2 cleavage site of the F gene of APMV-1 isolated from pigeons in India was attempted for pathotype prediction and determination of molecular epidemiology. Six pigeon origin NDV isolated in India between 1991 and 2001 were selected for this study. A portion of NDV F gene including the cleavage site was amplified by Polymerase Chain Reaction (PCR) and sequenced directly. The total number of nucleotide substitution among all six isolates ranged from 6 to 20; whereas, only four amino acid substitutions were observed. Nucleotides at position 304 and 357 were unique to all the pigeon isolates. The cleavage-activation site (380-397) had no nucleotide substitution and all the six pigeon isolates shared the amino acid sequence 112RRQKRF117 as that of velogenic viruses. The results of this molecular characterization study of Indian PPMV-1 isolates would help design better prevention and control measures for this important pathogen.
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