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Articles by J.M. Al-Khayri
Total Records ( 2 ) for J.M. Al-Khayri
  J.M. Al-Khayri
  Micropropagation systems often require optimization for different genotypes by modifying culture medium. Improved regeneration in numerous plant species was attained using Coconut Water (CW) as a medium additive. The objective of the study was to evaluate callus growth and somatic embryogenesis of two commercial cultivars of date palm (Phoenix dactylifera L.) in response to CW concentrations and sterilization methods, coautoclaving or microfiltration. Calli induced from shoot tip explants, were grown on multiplication medium supplemented with CW at 0 to 20% (v/v). To induce somatic embryogenesis, callus was transferred to a hormone-free regeneration medium containing the corresponding CW concentrations. Results have shown improved callus growth and enhanced somatic embryogenesis in response to CW. Callus fresh weight and numbers of resultant embryos were directly proportional to CW concentration reaching optimum levels at 10 and 15% CW for cv. Khasab and cv. Nabout Saif, respectively. Both coautoclaving and microfiltering CW were equally effective in promoting culture growth and differentiation; however, coautoclaved CW generally gave increased responses. Furthermore, CW enrichment expedited the induction somatic embryos and increased germination percentage. Potentially this may contribute to reducing the cost of commercial micropropagation of date palm.
  W.F. Shehata and J.M. Al-Khayri
  The objective of this study was conducted to establish a micropropagation system suitable for Hassawi peach (Prunus persica L.), an endangered landrace variety adapted to the hot arid climatic conditions of Al-Hassa, Saudi Arabia. This study examined the effect of some plant growth regulators on the response of node explants. The best shoot multiplication and growth was obtained using MS medium supplemented with 0.5 mg L-1 6-benzylaminopurine (BA) and 0.1 mg L-1 Indole-3-butyric acid (IBA). This treatment gave the highest shoot multiplication (5.8 shoots per explant), shoot elongation (4.7 cm) and leaf number (19.3 leaves per shoot). During rooting stage, the best rooting was associated with shoots cultured on ½ MS medium containing 0.5 mg L-1 IBA. This treatment gave 92% rooting with maximum root length of 6.8 cm and highest average number of root (5.4 roots per shoot). Plantlets exhibited normal growth and phenotype upon transfer to soil with 95% survival. This micropropagation system could contribute to the conservation and restoration of this threatened peach germplasm.
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