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Articles by J.L. Han
Total Records ( 4 ) for J.L. Han
  H. Zhang , T.W. An , J.W. He , Y.Z. Luo and J.L. Han
  Domestic yak plays a critical role in supporting the livelihoods of nomads in the Central Asian Highlands. Tianzhu White yak is a unique breed developed from a very small number of mutant founders and its white hair has a special niche market value. In this study, the genetic polymorphisms in Tyrosinase (TYR) gene, which has been considered as a ‘albino locus’ in cattle, were identified and characterized to search for alleles associated with the white coat colour in yak. A total of 973 yak samples were collected, including 438 animals from five nucleus breeding herds and 365 individuals from four reproductive herds of the Tianzhu White yak. The reference TYR genomic DNA sequence derived from a Hereford bull was used to design all primers for screening and sequencing the exon 2 and the last partial 5’-untranslated region (5’-UTR) of the yak TYR gene. Both PCR-SSCP analysis and DNA sequences for their complete exon 2 in selected samples from the Tianzhu White yak herds and black yak populations identified a single conserved sequence identical to other cattle breeds. There were 14 genotypes and seven alleles defined by nucleotide polymorphisms present in the 215 bp long 5’-UTR of yak TYR gene among all yak samples, of which five alleles were specific to yak while the other two alleles were of a cattle origin. Although, current data suggested no association of these polymorphisms with the yak coat colour variations, they shed light on the potential function of the promoter on regulation of expression of yak TYR gene that is warranted to screen for additional polymorphisms in its extended 5’-UTR and other exons.
  X.X. Li , L.X. Han and J.L. Han
  Chicken BLB1 and BLB2 genes are duplicated within MHC-B region that plays a crucial role in disease resistance or susceptibility. To investigate the genetic polymorphism in chicken BLB genes, we analyzed the complete genomic DNA sequences of BLB1 and BLB2 genes from 14 published MHC-B haplotypes (59 kb). Two pairs of primers were chosen or designed to amplify the exon 2 fragments of both genes from six known MHC-B haplotypes. The PCR products were directly sequenced for a preliminary identification of specific variations that were further validated using cloning approach. We found that the specificity of the primers becomes ambiguous to nearly all of the MHC-B haplotypes. Therefore it is impossible to design specific primer according to the complete exon and intron sequences for an independent amplification of complete exon 2 of the BLB1 or BLB2 genes. This calls for an alternative strategy for the investigation of genetic variations in the BLB genes.
  H.Y. Zhang , W.J. Yang , Y.Z. Luo and J.L. Han
  The widely expressed chicken UBTD2 gene in different types of tissues from embryonic to adult developmental stages implies its important role in regulating protein ubiquitination and delivery of ubiquitinated substrates, however, there is no specific study on the genomic DNA structure and function of chicken UBTD2 gene except a few invalidated SNPs derived from ESTs. In this study, a 1.2 kb long fragment within intron 2 of chicken UBTD2 gene was amplified and directly sequenced from a flock of 11 White Leghorn chickens. A total 15 sequences were identified from these birds, of which seven were homozygous but the remaining four were heterozygous. These new sequences were analyzed together with the homologous region in Gallus gallus reference genome version 71.4 (Chromosome 13: 8104760 - 8105979 in Galgal4). There were 10 polymorphic sites including seven transitions and three transversions, which defined four haplotypes in these 16 sequences. The Gallus gallus reference sequence formed a specific and distinct haplotype while the White Leghorn chickens carried three haplotypes at very similar frequencies of four, five and six sequences each. These data were the first piece of evidence of genetic polymorphisms present in the chicken UBTD2 gene which is therefore warranted for further investigation on the functional diversity in its complete genomic DNA sequence of different genetic backgrounds.
  B.P. Wang , D. Zhang , Y.Y. Liu , F. Wang , S.Y. Wang , L.D. Han , C.Y. Liu , C.X. Liu , J.P. Liu , J. Pan , W.B. Zhang , Tuo Ya , Zhaori Getu , Daolema , C.H. Huang , J.L. Han , Suya , L.G. Zhang , H.M. Zhou and L. Zhang
  The current study investigated the ovarian response to gonadotropin for establishing a suitable protocol of superovulation in Bactrian camel. Fifteen female camels were randomly divided into 4 groups to compare 4 different superovulation protocols during the natural breeding season. Each camel in 4 groups was injected with FSH at 80, 80, 60, 60, 60, 60, 40 and 40 mg, respectively total dosage of 480 mg, for consecutive 4 days at 12 h intervals. The camels in group 2-4 were naturally mated and subsequently injected 300 IU LH 48 h after the last injection of FSH, the camels in group 1 received the same procedure exception of LH injection. Ovarian follicles and corpora luteas were observed through synchronistic laparotomy 7-9 days after natural mating. The results indicated that there is no significant difference in average ovulation rate (p>0.05) among group 1-3 nor among group 2-4. However, there is considerable difference between group 1 and 4 in average ovulation rate (p<0.05), among which the value is highest (81.38±6.44%) for group 4 but lowest (16.89±7.98%) for group 1. Furthermore, the average number of follicles has yet no obvious difference (p>0.05) among group 1-3 nor among group 2-4 but significant difference (p<0.05) between group 1 and 4. Comparatively the animals in group 1 yielded highest average number of follicles (9.80±1.50). Conclusively, the protocol 4 had a best superovulatory effect with an average corpora lutea 9.33±1.45 and therefore it can be used for superovulation in camels.
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