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Articles by J.H.P. Sidadolog
Total Records ( 4 ) for J.H.P. Sidadolog
  Sri- Sudaryati , J.H.P. Sidadolog , Wihandoyo , W.T. Artama and D. Maharani
  Insulin-like growth factor binding protein 2 regulates a broad spectrum of biological activities involved in growth, development and differentiation. The current study was designed to investigate the associations of IGFBP2 gene polymorphisms with the variance components and genetic parameters of four weekly intervals of growth rate (0-4, 4-8, 8-12 weeks) of the Kampung chicken that were evaluated with classical models of quantitative genetics. Thirty two females and 16 males Kampung chicken were genotyped with IGFBP2 gene using PCR-RFLP method. Retrieved 3 genotype (CC, CT and TT) and 2 alleles (C and T). The influence of average effect of the C allele was greater than T allele on a 4-8 weeks intervals of growth rate, meanwhile for the males turn into 0-4 and 8-12 weeks interval growth rate. Ratio component additive and dominant deviation to each genotype is always equal for each interval growth rate. Variance component from heterozygote nearly zero when frequency of homozygote nearly equal and ratio variance dominance were opposite to allele frequency. Narrow-sense heritability based on genetic variance component of 0-4, 4-8 and 8-12 weeks interval growth rate for female were 0.01, 0.47 and 0.87 while for male chicken were 0.94, 0.07 and 0.12, respectively. It was concluded that IGFBP 2 gene association with growth rate on 4-8 and 8-12 weeks on females but in males only effect on growth rate on 0-4 weeks.
  S. Johari , N. Setiati , J.H.P. Sidadolog , T. Hartatik and T. Yuwanta
  The aim of this study was to determine the gene effect of Growth Hormone (GH) on divergent selection of Japanese quail. Quails were grouped into high weight (Q-H), low weight (Q-L) and random weight (Q-R) females as a treatment for divergent selection. Parameter phenotype observed in each generation is the weight at four weeks of age and egg production at ten weeks of age for five generations. The results showed that the dominance level on body weight of Q-L was incomplete dominance, Q-R was over dominance and Q-H was lack of dominance. While the dominance level on egg production of Q-L and Q-H were over dominance and Q-R was lack of dominance. The gene effect of GH on body weight of Q-H is 1.53 times greater than the Q-L and 12.37 times greater when compared with Q-R. Whereas the gene effect of GH on egg production of Q-H is 1.53 times greater than the Q-L but only 4 times greater when compared with Q-R. Should be developed that to increase the low-weight (Q-L) is in the BB genotype and the high weight (Q-H) is in the AA genotype groups. Otherwise, to increase the low-weight (Q-L) and high-egg production are in the AA and BB genotypes and the high weight (Q-H) and low-egg production are in the AA and AB genotypes groups.
  B. Ariyadi , J.H.P. Sidadolog , S. Harimurti , S. Sudaryati and Wihandoyo
  This study was conducted to analyze the relationship between the condition of non-feather distributions and its genetically traits as a multiple allele of the feather condition in the Indonesian indigenous naked neck chickens. Eight males Indonesian naked neck chickens were paired with the five females that had the different condition of non-feathers distribution, namely non-feathers on the neck only (30%); non-feathers on the crop and breast (60%) and non-feathers on the neck, crop, breast and the back of body (90%). Assuming that the non-feathers distribution was caused by a multiple alleles of Na gene and developing of distribution were caused by Na+ as a multiple alleles of Na gene. This study revealed the effect of multiple allele Na+ on the distribution of non-feather areas in the chicken bodies. Analysis of inheritance was conducted by Mendelian heredity with chi-square (X2) analysis. The results, in the Indonesian naked neck chicken, showed that the distribution of non-feathers areas from the neck to the back of body might be caused by genetic of Na+Na+ and Na+Na. The non-feather distribution areas in the crop, breast and the thigh might be genetics effect of Na+na and NaNa (90%). The non-feather distribution areas in the neck or in the crops might be genetics effect of Nana. The normal feathers of chicken might be effect of recessive gene of nana. Moreover, the study showed that the Indonesian naked neck chicken had a lower productivity, higher embryonic mortality and lower hatchability.
  B. Ariyadi , J.H.P. Sidadolog , S. Harimurti , S. Sudaryati , Wihandoyo and H. Sasongko
  Background: The cecum of the chicken gut may be susceptible to pathogens because it is readily colonized by microbes. The lower segment of the gut is also the primary tissue that permits the invasion of microorganisms from the external environment and the cloaca. Mucins, which are composed of glycoproteins, play significant roles in forming the barrier against infection on the mucosal surface. Objective: The aim of this study was to determine the differences in the mucosal barrier of the lower segment of the gut between Indonesian naked neck chickens and normal feathered chickens. Methodology: The lower segments of the gut (rectum, colon and cecal tonsil) of Indonesian indigenous naked neck chickens and normal chickens were collected. The expression of the mucin 2 gene in the gut mucosa was analyzed by reverse-transcription-polymerase chain reaction (RT-PCR). The localization and molecular sizes of the mucosal glycoproteins were analyzed by Western blot. Wheat Germ Agglutinin (WGA) and jacalin lectins were used for Western blot analysis. Results: The mucin 2 gene was expressed in the mucosal gut of the rectum, colon and cecal tonsil in both naked neck chickens and normal chickens. Western blot analysis showed a single band for both WGA and jacalin from the mucosal gut of the rectum, colon and cecal tonsil in both naked neck chickens and normal chickens. Conclusion: These results suggest that the mucin 2 gene and glycoproteins containing WGA and jacalin positive sugars cover the surface of mucosal gut in both naked neck chickens and normal chickens, most likely to form a mucosa barrier.
 
 
 
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