Asian Science Citation Index is committed to provide an authoritative, trusted and significant information by the coverage of the most important and influential journals to meet the needs of the global scientific community.  
ASCI Database
308-Lasani Town,
Sargodha Road,
Faisalabad, Pakistan
Fax: +92-41-8815544
Contact Via Web
Suggest a Journal
Articles by J.F. Kennedy
Total Records ( 4 ) for J.F. Kennedy
  M.C.B. Pimentel , A.B.F. Leao , E.H.M. Melo , W.M. Ledingham , J.L. Lima Filho and J.F. Kennedy
  The immobilization of Penicillium citrinum lipase by covalent bond onto ferromagnetic azide-Dacron showed 73% of specific lipase activity retention with 7.3 mg protein/g support. There was no change in either optimal temperature (37°C) or pH (8.0-8.5) after immobilization. The thermal stability of ferromagnetic azide-Dacron-Penicillium citrinum lipase derivative was significant, retaining 86.8% of lipolytic activity while the soluble enzyme retained only 34.2% after 1 h at 45°C, this represented an improvement of 154% in the thermal stability. This lipase did not show inhibition by isopropanol. Both soluble and immobilized Penicillium citrinum lipases showed a kinetic of Michaellis-Menten to hydrolysis of 4-nitrophenyl palmitate of (4NPP) at 37°C and pH 8.0. The values of KM were for soluble enzyme of 233 μM and immobilized derivative of 276 μM. This immobilized derivative showed a good operational stability keeping about 75% of initial activity after its reuse for 5 times. Also, it was stable to storage at 4°C in Tris-HCl buffer pH 7.2 with a half-life of 25 days. Immobilized Penicillium citrinum lipase was able to catalyse the synthesis of triolein using glycerol and olive oil as substrates free of organic solvent, with 80% of conversion after 20 h at 40°C.
  R. Panesar , P.S. Panesar , R.S. Singh , J.F. Kennedy and M.B. Bera
  The use of Kluyveromyces marxianus NCIM 3465 for the production of ß-D-galactosidase is reported. Experimentation was carried out to optimize the process parameters for maximum enzyme production by varying the parameters such as pH of the medium, temperature, inoculum size, age of inoculum, agitation and incubation time. The maximum enzyme activity was observed with pH 5.0, temperature 30°C, inoculum size 6% (v/v) having 20 h age, under shaking conditions (100 rpm) after 24 h of incubation.
  M.M. Camargo de Morais , S.A.F. Ramos , M.C.B. Pimentel , E.H.M. Melo , M.A. Morais Jr , J.F. Kennedy and J.L. Lima Filho
  A Brazilian strain of Penicillium citrinum produced a lipopolysaccharide with emulsifier properties during cultivation on mineral medium, containing ammonium sulfate as nitrogen source, with 1% (v/v) olive oil as the carbon source. The maximal emulsifier production (1.6 U mL-1) was obtained after 60 h of cultivation and the biomass reached 7.5 g L-1 at the end of fermentation. The production yield i.e. the amount the carbon source utilized for product synthesis was 54% and the best emulsifying activity was observed for xylene and diesel oil when compared to other carbohydrates tested. The emulsifier was shown to be stable to a wide range of pH and temperature values and was shown to contain D-galactose, D-glucose and D-xylose (8.2:1.0:5.3) with a total carbohydrate content of 43%. The presence of salts stimulated the emulsification activity, suggesting potential for its application in industrial waste or marine remediation.
  R.S. Singh , B.S. Sooch , Kamaljit Kaur and J.F. Kennedy
  The present study was carried out at flask level for the fermentative production of citric acid from cheddar cheese whey using a yeast culture Metschnikowia pulcherrima NCIM 3108. Clarified and unclarified whey with and without nutrient supplementation was screened for citric acid production. The order of citric acid production was supplemented unclarified whey>supplemented clarified whey>unclarified whey>clarified whey. The effect of various process parameters was investigated to maximize the citric acid production from supplemented unclarified whey. The lactose concentration of 6.0% (w/v) at pH 6.0 with an inoculum size 10% (v/v) of 48 h old culture at 30°C supported the maximum concentration of citric acid production after 5 days of incubation period. The agitation of the yeast culture suppressed the citric acid production to a little extent as compared to stationary conditions.
Copyright   |   Desclaimer   |    Privacy Policy   |   Browsers   |   Accessibility