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Articles by J. Singh
Total Records ( 5 ) for J. Singh
  J. Singh and K.N. Tiwari
  The objective of this study was to establish mass multiplication protocol for Clitoria ternatea. An efficient protocol for in vitro propagation of the valuable medicinal and forage plant aparajita (Clitoria ternatea L.) was developed through axillary shoot proliferation. Cotyledonary node having cotyledons or devoid of cotyledons were evaluated for axillary shoot development. The effect of cytokinins, sucrose concentrations and light-dark condition on axillary shoot regeneration was investigated. Cotyledonary node with cotyledons cultured on MS medium supplemented with 1.5 mg L-1 BAP yielded the maximum number (10.8) of shoots per explant. All media supplemented with 3% sucrose and incubated under 16 h light condition found most suitable for high frequency axillary shoot proliferation. Elongated shoots produced profuse rooting after transfer to half-strength MS medium supplemented with 0.2 mg L-1 IBA. The rooted plantlets were successfully acclimatized and exhibited 95% survival after transfer into the field. This protocol is suitable for large scale production of plantlets of C. ternatea.
  V.K. Gupta , J. Singh , R. Kumar and A. Bhanot
  The aim of this research was to study the pharmacognostic parameters of leaves of Ocimum gratissimum L. (family: Lamiaceae). Macroscopic study showed that the leaves of this plant possess dark green color with characteristic aromatic odour and bitter taste. Powder microscopy showed the presence of numerous glandular simple trichomes of average length 101 μm and anomocytic stomata were also present, whereas, T.S of leaf showed that the leaf has a pot shape midrib and a thin lamina with uneven lower epidermis attached at the lateral sides of its upper side leaving a concave central dorsal depression. Both upper and lower epidermis showed simple, covering, uniseriate trichomes as well as sessile short stalked glandular trichomes. Quantitative microscopy viz., veinlet termination number, vein islet number, stomatal number, stomatal index; physico-chemical parameters viz., ash values, extractive values, Thin layer chromatography (TLC); florescence analysis of Ocimum gratissimum L. were also been determined. TLC studies showed the presence of various phytochemicals in the extracts. Qualitative phytochemical analysis revealed the presence of alkaloids, tannins, flavonoids, terpenoids (methanolic, ethanolic extracts); alkaloids, flavonoids and terpenoids (petroleum ether, chloroform extracts) and carbohydrates in alcoholic extract. This is the first such study on standardization of Occimum gratissimum L. leaf which would serve as a standard reference for identification and distinguishing the plant from its adulterants.
  K.N. Gupta , V.K. Baranwal , B.K. Prasanna , J. Singh , Q.M.R. Haq and K. Gopal
  A study was undertaken to find out the genomic variability in Citrus yellow mosaic virus (CMBV), a bacilliform virus under the genus Badnavirus, infecting different citrus species in India and their phylogenetic relationship with other badnaviruses. Comparison of genome sequences of four isolates of CMBV infecting different species of citrus with previously sequenced three CMBV isolates indicated variability in coding region of ORFs 1, 2 and 3 of all the CMBV isolates infecting same or different citrus species with highest variability in coding region of ORF 3. Coding region of ORF 4, 5, were also highly variable in CMBV isolates but they were highly conserved in CMBV isolates infecting Acid lime. ORF 6 was comparatively conserved and was identical in CMBV isolate infecting Acid lime. All the CMBV isolates shared maximum identity with Cacao swollen shoot virus (CSSV) in ORF 1 and 3 indicating that CMBV isolates are more closely related to CSSV than other badnaviruses. This study has implication in determining the diversity and diagnosis of CMBV.
  J. Singh , S. Gupta and H. Kaur
  Drug release from polymers may be erosion or diffusion based or combination of these processes. Various equations and models are available for relating drug release. Most of the decisions are made on basis of similarity of original and predicted release profiles (predicted by fitting release data in various models or equations). In this study, decision for release pattern has been made on basis of SSR/R2 for model drug ofloxacin from laboratory developed Extended Release (ER) matrix tablets. Three batches A, B, C having HPMC, sodium alginate and 50:50% mixture of HPMC:sodium alginate were prepared. The USP Dissolution apparatus 2 was used for ofloxacin release and swelling-erosion studies of different batches in SGF, buffer of pH 6.2 and SIF. The batch C, ofloxacin matrix tablet composition was finalised on basis of <30% release within 1 h in SGF, >85% release upto 8 h in SIF and conformity to shape of profile within 1-7 h dissolution in buffer of pH 6.2. Instead of use of SSR and R2 on individual basis, SSR/R2 expressed ofloxacin release mechanisms in good agreement to physically observed swelling-erosion behaviors of matrix tablets in all pH conditions.
  H.P. Singh , S. Singh and J. Singh
  The speech signal is degraded due to delay, jitter and packet losses, when passed through different network layers of internet protocol based Voice over Internet Protocol (VoIP) system. The speech quality of the VoIP system can be improved by reducing the background noise, codec distortions and various network impairments such as packet loss, delay and jitter. The work in this paper proposes the Finite Impulse Response filter (FIR) based scheme for speech quality improvement of the VoIP signal. The lab experiment is performed to realize the VoIP system and to obtain the degraded database. For various noisy conditions, the performance of the enhanced VoIP signal is evaluated using Perceptual Evaluation of Speech Quality (PESQ) measurement for narrowband signal. The results show much improvement in speech quality with proposed method.
 
 
 
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