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Articles by J. Safiah
Total Records ( 6 ) for J. Safiah
  A.J. Memon , A.D. Talpur , M.I. Khan , M.O. Fariddudin , J. Safiah , A.B. Abol-Munafi and M. Ikhwanuddin
  The objectives of this study were to determine the effects of different cryoprotectants on sperm viability and optimization of spermatophore cryopreservation protocol for durable storage of Banana shrimp (Penaeus merguiensis). Spermatophore suspended for 15 min in Calcium-Free saline (Ca-F saline), used CPA MgCl2 and with concentration (15%), thawing temperature was 27°C. Use 15 min equilibration in room temperature (25°C) overall. Exposure and cooling rate selected as 25, 20, 16, 4, 2, -4, -20, -80, -150°C/10 min. Examination of sperm viability used a modified eosin-nigrosin staining technique. The smallest reductions in apparent sperm viability occurred with MgCl2, however freezing protocol was developed using Ca-F saline containing 15% MgCl2. Spermatophores were cryopreserved using above exposure/cooling rate and -196°C in liquid nitrogen up to 180 days. Mean sperm viability for fresh (93.8±1.3%) and cryopreserved spermatophore held for 24 h and 60 days was 83.5±0.6 and 61±1.2 did not differ (p>0.05), however that for spermatophore stored in liquid nitrogen between 90 and 180 days were lower (p<0.05) and varied from 55.4±0.3-16.4±1.2. Spermatophores earlier held in liquid nitrogen for 60 and 90 days. However, storage beyond 90 days caused a significant decline (p<0.05) in sperm viability. Spermatophores kept for 120 and 150 days had viabilities of 48.9±0.9 and 32.4±0.9%, respectively. Cryopreserved spermatophore stored in liquid nitrogen from 150-180 days had low viabilities (<35%). Mean fertilization rate of P. merguiensis females artificially inseminated with cryopreserved spermatophore that had been stored in liquid nitrogen for 7-30 days and for 60-90 days were 73.9±1.5-66.7±3.1 and 67.3±3-64.1±2.1%, respectively whereas that of fresh spermatophore was 88.2±1.5%. Hatching rates of eggs fertilized with cryopreserved spermatophore kept for 7-30 days and for 60-90 days were 77.6±2.5-72.7±3.5 and 81.5±12.1-62.5±1.5 which were not different (p>0.05) from those of the control group 76.2±13.5%, respectively. In conclusion, Cryopreserved spermatophore held in liquid nitrogen l<90 days revealed high sperm viability although, for longer periods, sperm viability declined at 180 days.
  S.N. Fatihah , J. Safiah , A.B. Abol-Munafi and M. Ikhwanuddin
  The study was aimed to determine the biochemical changes of total protein, glucose, lactate dehydrogenase (LDH) and total lipid in the cryopreserved sperm of mud spiny lobster, Panulirus polyphagus. The mean sperm viability, total protein, glucose, LDH and lipid for control and cryopreserved sperm that stored in liquid nitrogen from control, 6, 12, 24 h, 7, 30 and 60 days were 94.91±2.17% (control), 80.58±1.33, 77.08±2.53, 76.49±3.10, 63.32±7.08, 62.93±3.72 and 60.91±10.78% (sperm viability), 21.44±3.52, 18.82±0.23, 17.79±0.80, 16.98±0.48, 16.65±0.07, 15.03±2.53 and 12.16±2.02 mg mL-1 (total protein), 0.28±0.012, 0.26±0.004, 0.20±0.035, 0.15±0.062, 0.11±0.036, 0.07±0.036 and 0.04±0.013 mg mL-1 (glucose), 72.65±7.88, 64.38±6.40, 64.24±4.80, 62.84±7.20, 62.11±3.84, 49.94±2.78, 38.31±3.29 IU L-1 (LDH) and 8.77±0.05, 4.87±0.25, 4.69±0.29, 4.37±0.02, 4.16±0.17, 2.87±0.29 and 2.83±0.26 mg mL-1 (total lipid). There were significant differences in sperm viability, total protein, glucose, LDH and total lipid between cryopreserved periods (p<0.05). As a conclusion, the sperm viability and biochemical changes of total protein, glucose, LDH and total lipid were determined in the cryopreserved sperm for control, 6, 12, 24 h, 7, 30 and 60 days. The storage duration in P. polyphagus cryopreserved sperm was also determined until 60 days and suitable for further breeding program.
  S.N. Fatihah , J. Safiah , A.B. Abol-Munafi and M. Ikhwanuddin
  The present study aimed to determine the effect of testosterone undecanoate hormone on sperm quality (sperm viability) and sperm quantity (sperm counts) and its levels in the hemolymph of male mud spiny lobster, Panulirus polyphagus. Male P. polyphagus was injected laterally in fifth abdominal segment of pure hormone, Testosterone Undecanoate (TU) and ethanol at days 1, 8, 15, 22 and 29. Hemolymph of P. polyphagus was taken every two weeks and checked with Enzyme-linked immunosorbent assay (ELISA) to measure hormone levels. The mean sperm quality and quantity were increased due to increase the TU dose and TU levels also increase. The sperm quality, quantity and hormone levels were relevance each others. These findings indicate that TU injection should be evaluated as a practical way of improving sperm quality and quantity in commercial operations.
  S.N. Fatihah , J. Safiah , A.B. Abol-Munafi and M. Ikhwanuddin
  The present study aimed to determine the effect of 17α-hydroxyprogesterone (17α-OHP) and 17α-hydroxypregnenolone (17α-OHPL) on sperm quality and sperm quantity in male mud spiny lobster (Panulirus polyphagus). The mean of sperm quality was increased in 17α-OHP and 17α-OHPL treated hormones. In 17α-OHP injected animals, the mean of sperm quantity of dose 0.01 μg g-1 b.wt. was increased than 17α-OHPL. Meanwhile, 17α-OHP and 17α-OHPL concentrations were lower when injected with the hormones but 17α-OHP was higher at only day 15 (dose 0.01 and 0.1 μg g-1 b.wt.). For 17α-OHPL, the hormone was a prohormone in the body of P. polyphagus and only required smaller to increase the sperm quantity. Besides, when the higher dose of 17α-OHPL (0.1 μg g-1 b.wt.) was used in P. polyphagus, the development of P. polyphagus was inhibited and decreased the sperm quantity and 17α-OHPL concentration in hemolymph was lower. Injection of 17α-OHP in P. polyphagus has increased the sperm quality and quantity for both 17α-OHP dosage of 0.01 and 0.1 μg g-1 b.wt. However, injection of 17α-OHPL in P. polyphagus has decreased the sperm quantity only, also for both dosage of 0.01 and 0.1 μg g-1 b.wt. and lower on hormone concentration.
  A.J. Memon , M. Ikhwanuddin , A.D. Talpur , M.I. Khan , M.O. Fariddudin , J. Safiah and A.B. Abol-Munafi
  The study was conducted to every hour assess the sperm viability of banana shrimp (Penaeus merguiensis) collected from Kedah water, West Malaysia (5°39'N; 100°19'E). Evaluations were done on three ways: group A: whole fresh specimens maintained at 2°C prior to extraction, group B: whole spermatophore maintained at 2°C and C: sperm suspension maintained at 2°C. Spermatophores counts were determined by sperm suspension using modified eosin-nigrosin staining method. Percentage of mean sperm viability for the fresh sample in group A at time zero was 93.96±3.74%, B was 98.8±0.7% and C was 99.02±0.7%. In group A, viability of the sperm considerably decreased after the first 60 min was 76.4±5.96% but in group B and C was gradually decreased at 91.4±0.9 and 97.6±1.2%. These were 67.9±5.13 and 62.5±5.1% in group A, 82.3±1.4% and 75.9±2.2% in group B and in group C was 93.3±1.9 and 88.2±3.1%, respectively after 1st-2nd h times elapsed. At 7th h in all groups the viability decreased significantly to <60% (p<0.05). Following, mean sperm viability were considerably decreased to group A was 50.7±4.79 and 37±6.52%, group B was 51.2±1.6 and 31.6±1.4% and group C was 56.2±1.9 and 41.5±2.3%, respectively after 7th and 9th h. However, for spermatophores in group A after 7th h fertilization and hatching rate was 44.3 and 64.4% in group B was 61.9 and 67.7% and group C was 42.9 and 61.3%, respectively at rates comparable to control 88.2 and 76.2%, respectively. There was no significant relationship was observed between biomass of the spermatophore to the body weight of the shrimp (p>0.05). The present study also revealed that specimens, spermatophore or sperm suspension maintained at 2°C could be utilized for fishery management through artificial insemination process till 7th h.
  A.J. Memon , N. Hidayati , A.D. Talpur , M.I. Khan , M.O. Fariddudin , J. Safiah , M. Ikhwanuddin and A.B. Abol-Munafi
  In aquaculture industry, spermatophore transfer technique such as artificial insemination in particular with reference to banana shrimp P. merguiensis is challenging. The aim of this study is to examine a novel technique for artificial insemination in P. merguiensis using SHDAI (Shrimp Holder Device for Artificial Insemination). In order to transfer the spermatophore properly into thelycum, an appropriate shrimp holder with continuous aeration system has been developed. During the process of manual spermatophore transfer (artificial insemination) also, a protective device to keep the female P. merguiensis shrimps alive and under minimum stress is necessary. The artificial insemination process carried out using SHDAI showed no signs of stress and/or mortality of the broodstock. During the experiments, female shrimps were 100% alive and active. Altogether, 78 female shrimps were tested of which 63 successfully accepted the spermatophore by using SHDAI. The accepted spermatophore percentage was significantly higher and achieved as 80.76%. Accepted spermatophore mass in the frozen, control and mean of both were recorded as 48.36±10.45, 45.0±15.28 and 46.68±2.38% which indicated no significant differences (p<0.05) However, frozen sperm at -196°C LN up to 90 days was 64.1±4.3% which indicated no significant differences between the SHDAI and control (p<0.05). Whereas frozen sperm at 196°C up to 90 days in LN was 62.5±2.9% there was no significant difference between the SHDAI and control (p<0.05). In the present study, overall quality of sperm in terms of the fertilization rate and hatching rate were almost similar between inseminated (using SHDAI) and control (natural mating) broodstock.
 
 
 
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