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by
J. Prastowo |
Total Records (
4 ) for
J. Prastowo |
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J. Prastowo
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A. Sahara
,
C. Marganingsih
and
B. Ariyadi
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In Indonesia, pigeon is one who is familiar with humans, beside can be consumed, usually maintained for hobby or pleasure. One of the parasitic worms that infect the pigeon's kidneys was Paratanaisia bragai (P. bragai). That worm can cause pathological changes in the renal pigeons. Evaluation of renal function can be done through checking of serum creatinine and urea. This study was conducted to identify the renal parasite and its blood urea-creatinine profile on the Indonesian indigenous pigeons. Fifty eight Indonesian indigenous pigeons were identified for renal parasite. Then all of the collected blood was evaluated for urea and creatinine levels. The difference values of urea and creatinine between infected and uninfected renal pigeon were analyzed by t-test. Fifty one percent (30/58) of them were infected by renal parasite. Identification of renal parasite showed the P. bragai. The creatinine levels in infected pigeons (2.650±1.7228) mg/dL were significantly higher than uninfected pigeons (1.732±1.2944) mg/dL. The blood urea levels showed non significant difference between the infected pigeons (7.7700±3.09775) mg/dL and the uninfected pigeons (6.4364±3.60415) mg/dL. The increase of urea and creatinine levels might be not correlated with the number of worms, since the Pearson correlation test showed no significant results. These results will be better if accompanied by histopathology of kidney, uric acid levels and other blood components. |
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J. Prastowo
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A. Sahara
,
I. Widayati
and
B. Ariyadi
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This study was conducted to identify the type of Acanthocephala that invaded in the small intestine of laying hens based on morphological examination with Semichon’s Carmine staining. A total of 157 laying hens aged over 50 weeks were used. Worms were collected from the intestine, stored in distilled water at 4°C for 2 days and then stained by Semichon’s Carmine for worm identification. Nineteen laying hens (12%) were infected by worms and 187 Acanthocephala were collected from posterior part of the small intestine. Morphological observation and measurement of worms were the proboscis, trunk, genital system and eggs. The identified Acanthocephala were creamy white without pseudo segmentation, proboscis shaped like a pear with hooks, spines and divided into anterior and posterior parts. The number of hooks on the anterior proboscis were 18-22 rows, each contains 5 hooks, long cylindrical trunk and tapered at both ends. The trunk had no spine with two long Lemniscus and it was not tied. The reproductive system of male worms comprised of testis, cement glands, vesicles gland and bursa of copulatory. The reproductive system of female worms comprised of the uterus and vagina. Eggs were oval shape with 3 layers of walls and contain embryos. Based on morphological examination of Acanthocephala worms, it was concluded that the species was Mediorhynchus gallinarum. |
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J. Prastowo
,
O. Herawati
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Bambang Ariyadi
and
Kurniasih
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Background and Objective: Ascaridia galli (A. galli) infection can depress the body weight and egg production of domestic chickens. Synthetic anthelmintics are usually used as a treatment but A. galli resistance to synthetic anthelmintics is rising. The aim of this research was to understand the effects of combinations of Areca catechu seed and Anredera cordifolia leaf on the number of eggs per gram (EPG) of feces, body weight, number of Ascaridia galli worms, histopathology and immunoexpression of CD4+lymphocytes in the intestine. Materials and Methods: Three groups of fifteen chickens infected with A. galli were used in this research. For seven days, group 1 was orally administered a combination of 2 mg kg–1 b.wt., of Areca catechu and 1 mg kg–1 b.wt., of Anredera cordifolia, group 2 was given a combination of 5 mg kg–1 b.wt., of Areca catechu and 2.5 mg kg–1 b.wt., of Anredera cordifolia and group 3 was not treated. This research had ethical clearance No. 00040/04/LPPT/IV/2017. On the 10th day, the chickens were euthanized and examined for the number of EPG of feces, body weight, number of A. galli, histopathology and immunoexpression of intestinal CD4+lymphocytes. The data were analyzed statistically and the histopathological changes were analyzed descriptively. Results: The results showed that the combination of Areca catechu and Anredera cordifolia significantly reduced EPG of feces and eliminated A. galli in 60% of the chickens in group 1 but there was no significant difference in body weight among groups. In group 2, the alkaloids and saponins contained in Areca catechu and Anredera cordifolia display antagonistic activity. Thus, high doses do not reduce EPG levels in group 2. Immunohistochemistry showed that wound healing is improved in the treated groups. Conclusion: Ascaridia galli infection might be reduced by a combination of Areca catechu and Anredera cordifolia. |
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J. Prastowo
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B. Ariyadi
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Background and Objective: Chickens infected with Ascaridia galli worms (A. galli ) suffer from slow growth and decreased weight gain. Infection with A. galli usually causes severe damage to the intestines due to migration of the worms in the tissue phase of the intestinal mucosa layer, which affects the proliferation of goblet cells in the duodenums of affected chickens. It is necessary to conduct a study that evaluates the effects of A. galli infestation on the number of goblet cells in the duodenal epithelium of infected chickens. Methodology: In this study, we used a total of 20day-old chicks (DOC). The A. galli worms used in this study were obtained from local chicken slaughterhouses. The worm eggs were embryonized to the L2 stage and inoculated with as many as 5000 eggs/chicken. Samples of the duodenum were taken after the chickens were 6 weeks old, at which time Periodic Acid Schiff (PAS) staining was performed. The elimination rate of the number of worm eggs after the infection was observed every once a week up to week 6 of the research period. Results were analyzed using Student’s t-test and descriptive analysis. Results: There tended to be a higher number of mucin-producing goblet cells in the treatment group than in the control group. The number of worm eggs found increased until the 21st day after infection. The peak of worm egg elimination occurred on the 21st day after infection, with a mean of 2000±250 eggs per gram (EPG); however, the number decreased again on the 28th day after infection. Conclusion: Based on the results of this study, it can be concluded that A. galli infection has a significant effect (p<0.05) on the increased number of goblet cells in the duodenal epithelium of broiler chickens. This worm infection also affects the length of the duodenum and the increase in chicken body weight on a weekly basis. |
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