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Articles by J. Pickova
Total Records ( 4 ) for J. Pickova
  T. Turner , A. Hessle , K. Lundstrom and J. Pickova
  Sixteen Swedish Red steers were fed a hempseed cake (HC) or soybean meal (SM) protein supplement. Lipid extract from fresh and cooked M. longissimus dorsi were analysed. Diet comparison showed that HC had increased monounsaturated fatty acid proportion, primarily 18:1 cis-9 in the meat (P<0.05). Additionally, HC steers had higher proportions of 18:1 trans-11 and 18:2 c-9, t-11 (P<0.05). Furthermore, HC steers had decreased n-6/n-3 fatty acid ratio compared to SM steers (P<0.05). Cooking increased polyunsaturated fatty acid (PUFA) and n-6 level, particularly 18:2n-6 and 20:4n-6 (P<0.05). Cooking decreased the proportion of 17:0 and 18:0 (P<0.05). In polar lipid, HC steers had lower saturated fatty acid (P<0.05) and higher PUFA levels (P<0.05). Warner-Bratzler shear force and lipid content were unaffected by dietary treatment. Cooking decreased the triacylglycerol level of both groups (P<0.01). We conclude that HC is a viable alternate supplement for beef diets which improves the meat FA profile.
  S. Sampels , T. Turner , Aring. Ostrom and J. Pickova
  In an earlier study, we concluded that pellet-fed reindeer could not elongate 18:3n - 3 (α-linolenic acid - ALA) sufficiently towards long-chain polyunsaturated fatty acids (LC PUFA) and need supplementation of LC PUFA. The present work investigated that the addition of n - 3 LC PUFA to feed in combination with ALA would increase the LC PUFA in the meat. Two groups of reindeer were fed pellets containing either linseed cake or linseed cake combined with algae (Nannochloropsis oculata) for 6 weeks before slaughter. Dietary n - 6/n - 3 ratio had a distinct influence on meat fatty acid (FA) composition when comparing linseed and linseed algae-fed animals with animals fed a conventional diet. Increased dietary proportions of ALA and eicosapentaenoic acid (EPA) increased these FA in muscle and docosapentaenoic acid (DPA) and docosahexaenoic acid in the polar lipid fraction compared to the conventional-fed animals. We concluded that an increased proportion of dietary EPA might lead to an increased elongation towards DPA in muscle. Algae and linseed are possible additives to reindeer feed in order to assure a similar valuable FA composition as in pasturing animals.
  Increased use of plant oils with different origins and quality in fish feed needs to be approached from a food safety and fish welfare point of view. Plant oils contain a number of bioactive minor lipid compounds that may affect the fish's metabolism and taste perception. This study focuses on the effect of replacing fish oil (FO) with different levels of cold-pressed rapeseed oil (RO) on the lipid composition in muscle and liver as well as on the preference by the fish. Rainbow trout (Oncorhynchus mykiss) were fed diets with a FO:RO ratio of 100:0, 75:25, 50:50 and 25:75 until twofold weight increase. In self-selecting feed trials of single rainbow trout, fish preferred the diet composed of only FO compared with the diets with RO but did not discriminate between different levels of RO. Plant sterols and their metabolites were found in liver of the fish fed RO diets, suggesting an effect on the sterol metabolism different from fish fed a 100% FO diet. The largest effects were seen in the fatty acid composition of the edible tissue of the fish with a decrease in 22:6n-3 and 20:5n-3 and an increase in 18:2n-6 and 18:1n-9.
  For the first time, pre- and post-hepatic plasma lipid profiles were monitored following a single meal in a free-swimming, non-anaesthetized fish. Rainbow trout (Oncorhynchus mykiss; 700–1500 g; 10 °C) were equipped with cannulae in the dorsal aorta (DA) and hepatic portal vein (HPV). Simultaneous blood samples, taken from both cannulae at 0, 3, 6, 12, 24 and 48 h postprandial, revealed the time course of the plasma lipid profiles following a single meal (1% of body mass). Primarily monounsaturated fatty acids with the exception of 18:1n − 9, increased significantly from baseline by 12 h postprandial without greatly affecting total plasma lipid concentrations. Total plasma lipids then showed a small peak at 24 h postprandial, coinciding with a peak in triacylglycerols. We conclude that assimilation of lipids from the digest into the plasma is slower than reported for proteins and carbohydrates in the same species. Furthermore, as there were no significant differences between the HPV and DA, no measurable effect of hepatic passage on plasma lipid levels was resolved. Therefore, we also conclude that, in contrast to that in higher vertebrates, hepatic passage does not seem to have a major role in rainbow trout for modulating the postprandial plasma profile of lipids.
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