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Articles by J. Koudou
Total Records ( 5 ) for J. Koudou
  C. Zongo , A. Savadogo , L. Ouattara , I.H.N. Bassole , C.A.T. Ouattara , A.S. Ouattara , N. Barro , J. Koudou and A.S. Traore
  The main goal of this study was to determine the phenol content, the antimicrobial and the antioxidant activities of acetone and water extracts from the rhizomes of Ampelocissus grantii (Baker) Planch (Vitaceae). The polyphenol content of extracts was determined colorimetrically using Folin-Ciocalteu method. The antioxidant activity of the same extracts was also screened using 1, 1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging method. The antimicrobial activity was performed by disc diffusion and broth microdilution assays against 9 reference bacterial strains including gram-negative and gram-positive bacteria and 1 fungal strain. The acetone extract exhibited the higher level of Total Polyphenol Contents (TPC) which correlated with strong antioxidant activity (IC50 = 10.87 μg mL-1 and AAI = 5.6) and antimicrobial activity. The polyphenol content of the acetone extract was 124.26 gallic acid equivalent/100 g (GAE/100 g), that of water extract was 74.25 GAE/100 g. The largest diameter of inhibition zone (DIZ) (15 mm) and the lowest Minimal Inhibitory Concentration (MIC) value (0.625 mg mL-1) were recorded with Enterococcus faecalis 10907 CIP and Bacillus subtilus ATCC 21332. This study is a scientific base on the use of this plant in traditional health cure system.
  J. Koudou , P. Edou , L.C. Obame , I.H. Bassole , G. Figueredo , H. Agnaniet , J.C. Chalchat and A.S. Traore
  The resin oil obtained by hydrodistillation from Dacryodes edulis G. Don growing in Gabon was analyzed by GC and GC/MS. The major constituents in the essential oil were sabinene (21.76%), terpinene-4-ol (19.79%), α-pinene (17.47%) and p-cymene (11.29%). The in vitro antioxidant activity was investigated with two methods: 2, 2-diphenylpicrylhydrazyl radical (DPPH) scavenging essay and β-carotene bleaching test. Butylated hydroxyltoluene was employed as positive control. The essential oil showed antioxidant and DPPH radical scavenging activities and it displayed the inhibition of lipid peroxidation. Furthermore, the antimicrobial activity of essential oil was evaluated using a broth microdilution method. Dacryodes edulis essential oil exhibited antibacterial activity but it was unable to inhibit the growth of fungal species tested.
  L. Ouattara , J. Koudou , C. Zongo , N. Barro , A. Savadogo , I.H.N. Bassole , A.S. Ouattara and Alfred S. Traore
  The main goal of this study was to determine the phenol content, the antibacterial and the antioxidant activities of the three species of Lannea largely use in traditional medicine in Burkina Faso. The total phenolic and flavonoid contents of hydro alcoholic extract (70%V/V ethanol/distilled water) from the barks of Lannea acida, Lannea microcarpa and Lannea velutina (Anacardiaceae) were determined by the method of Folin Ciocalteu and AlCl3 by spectrophotometry. These extracts were tested for their antioxidant and antibacterial activities. Antioxidant activity was determined by the method of DPPH and compared with quercetin. Antibacterial activity was performed by disk diffusion and broth microdilution essays against nine reference bacterial strains including gram-positive and gram-negative bacteria. L.acida exhibited the highest total phenolic contents (40.55±0.26 g GAE/100 g) which correlated with better antioxidant activity (IC50 = 345.72±7.76 μg mL-1). Furthermore the highest content of total flavonoids (11.02±0.04 g QE/100 g) and the largest anti bacterial spectrum (7.82 μg mL-1≤MIC≤ 62.5 μg mL-1) were recorded with L.velutina. These results show that the barks of L. acida and L.velutina could be used respectively as a potential natural antioxidant and antibacterial agent.
  J. Koudou , A.W. Etou Ossibi , K. Aklikokou , A.A. Abena , M. Gbeassor and J.M. Bessiere
  The composition of the essential oil from the fruit seeds of Monodora myristica Gaertn. (Annonaceae) was studied by capillary gas chromatography. The analysis using a combination of retention indices and combined Gas Chromatography/Mass Spectrometry (GC/MS) led to the identification of 30 components. The oil contains mainly monoterpenoids (93.2%) out of which 77.4% are monoterpene hydrocarbons and some sesquiterpenoids (5.8%) The major constituents were α-phellandrene (34.4%) and p-cymene (22.2%). The essential oil effects on the cardiovascular system were studied by recording the amplitude and frequency of the frog isolated heart contractions and by recording arterial blood pressure variations of the guinea pig. At the dose of 40, 80 and 120 μL kg-1 the essential oil induced a hypotensive effect on the blood pressure and at the dose of 0.01-0.05%. It reduced significantly the cardiac contractions of the isolated heart; however at 0.06% it totally stopped the cardiac contractions. Both these effects conducted in the present study could contribute to the anti hypertensive activity of the Monodora myristica essential oil.
  L. Ouattara , J. Koudou , D.S. Karou , L. Giaco , G. Capelli , J. Simpore , M. Fraziano , V. Colizzi and A.S. Traore
  The cytotoxic and anti-Mycobacterium tuberculosis H37Rv activities of hydro-alcoholic extract of Lannea acida A. Rich (Anacardiaceae) were assessed. The cytoxicity evaluation was carried out on THP1 monocytoid cell line (after 24 h at 1; 5 and 10 μg mL-1) and showed only a slight modification of lactate dehydrogenase (LDH) release. The rate of monocytes in different stages of mitosis had been amended in absence and presence of extract as follows: Go/ G1 58.83 - 59.83%; synthesis 21.95 - 18.64%; mitosis 16.67 - 15.77%; necrosis 2.65 - 5.64 %. The percentage of inhibition of Mycobacterium tuberculosis proliferation was respectively 77.6 and 36.8% at 1.2 and 0.6 mg mL-1 of extract. This is an interesting experimental study on antimicrobial and immune-stimulating properties of Lannea acida ethanol-water (70%v/v) extract which may contain potential antibacterial and immune-stimulating agents for clinical use.
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