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Articles
by
J. Mishra |
Total Records (
1 ) for
J. Mishra |
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Saurabh Singh
,
Asha Sinha
,
Shakshi Singh
,
Richa Raaj
and
J. Mishra
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Good quality seed is the prerequisite to agriculture and though
of such vital significance very less attention has been paid to their efficient
storage and storage losses. The present study was undertaken to screen for isolation
and percent incidence of seed borne mycoflora wherein two Mungbean varieties
viz. HUM-4 and HUM-12 were screened by standard blotter paper and agar plate
methods at the different period of storage. Seeds of Mungbean varieties were
obtained from agriculture farm, Banaras Hindu University, Varanasi. The surface
sterilization was done by 0.1% mercuric chloride (HgCl2) for 1 min
and washed thoroughly with distilled water. Both sterilized and unsterilized
seeds were used for detection of seed borne pathogen. A total of 22 different
fungi including one bacterial isolate belonging to 12 distinct genera were isolated
were from these seed samples. The percentage incidence of different fungi varied
with the storage periods. Aspergillus niger recorded at maximum level
in 22.33, 43.27, 49.95, 19.98, 22.98 and 39.00% in Agar plate method from fresh,
180 and 360 days period of storage in HUM-4 as well as HUM-12 genotype. Highest
incidence of seed borne fungi was recorded in genotype HUM-4 in Agar plate followed
by HUM-12 through blotter condition. In all storage period unsterilized seed
yielded most number of seed borne fungi as compared to sterilized seed. Result
revealed that in all storage period Aspergillus sp. Penicillium
sp. Curvularia lunata were recorded highest in HUM-4 through Agar plate
method, Fusarium sp. were recorded highest in HUM-4 blotter method and
lowest in HUM-12 agar plate methods, Rhizopus Cephaliofhora irregularis,
Chaetomium globosum and Cladosporium cladosporoids in HUM-12 under
Agar condition. Result showed that maximum number of fungi belonged to Deuteromycotina
(68. 98%) followed by Ascomycotina (13. 89%) and Zygomycotina (9. 09%). |
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