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Articles by J Nolte
Total Records ( 3 ) for J Nolte
  T Perl , E Carstens , A Hirn , M Quintel , W Vautz , J Nolte and M. Junger
  Background

We aimed to measure propofol concentrations in exhaled air with an ion mobility spectrometer coupled to a multicapillary column for pre-separation (MCC–IMS). In addition, we aimed to compare the values of these measurements with serum propofol concentrations, as determined by gas chromatography–mass spectrometry (GC–MS).

Methods

Thirteen patients, ASA I or II, undergoing elective ENT surgery were studied. Anaesthesia was induced with propofol 2.1 (0.7) mg kg–1, rocuronium 0.5 (0.1) mg kg–1, and remifentanil 0.5 µg kg–1 min–1. After tracheal intubation, anaesthesia was maintained with a continuous infusion of propofol 3.9 (1.8) mg kg–1 h–1 and remifentanil 0.5 µg kg–1 min–1. Simultaneously, a venous blood sample was obtained. Propofol concentrations in serum were determined by GC–MS and compared with the height of the respective propofol signals achieved by MCC–IMS.

Results

Twenty-four pairs of samples were obtained. The comparison of propofol concentrations in exhaled air and serum presented a bias of –10.5% and a precision of ± 12.3%. With these values, the 95% limits of agreement were 14.1% and –35.1%.

Conclusions

MCC–IMS may be a suitable method to determine propofol concentrations in exhaled air, and may be used to predict propofol concentrations in serum.

  U Zechner , J Nolte , M Wolf , K Shirneshan , N. E Hajj , D Weise , B Kaltwasser , A Zovoilis , T Haaf and W. Engel
 

Recently, several groups described the isolation of mouse spermatogonial stem cells (SSCs) and their potential to develop to embryonic stem cell (ESC)-like cells, so-called multipotent germline stem cells (mGSCs). We were the first to derive such mGSCs from SSCs isolated from adult mouse testis and, therefore, called these mGSCs multipotent adult germline stem cells (maGSCs). Here, we comparatively analyzed gene-specific and global DNA methylation profiles as well as the telomerase biology of several maGSC and male ESC lines. We show that undifferentiated maGSCs are very similar to undifferentiated male ESCs with regard to global DNA methylation, methylation of pluripotency marker gene loci, telomerase activity and telomere length. Imprinted gene methylation levels were generally lower in undifferentiated maGSCs than in undifferentiated male ESCs, but, compared with undifferentiated mGSCs derived by other groups, more similar to those of male ESCs. Differentiation of maGSCs increased the methylation of three of the four analyzed imprinted genes to almost somatic methylation patterns, but dramatically decreased global DNA methylation. Our findings further substantiate the pluripotency of maGSCs and their potential for regenerative medicine.

  S Meyer , J Nolte , L Opitz , G Salinas Riester and W. Engel
 

DNA microarray analysis was performed with mouse multipotent adult germline stem cells (maGSCs) and embryonic stem cells (ESCs) from different genetic backgrounds cultured under standard ESC-culture conditions and under differentiation-promoting conditions by the withdrawal of the leukemia inhibitory factor (LIF) and treatment with retinoic acid (RA). The analyzed undifferentiated cell lines are very similar based on their global gene expression pattern and show 97–99% identity dependent on the analyzed background. Only 621 genes are differentially expressed in cells derived from mouse 129SV-background and 72 genes show differences in expression in cells generated from transgenic Stra8-EGFP/Rosa26-LacZ-background. Both maGSCs and ESCs express the same genes involved in the regulation of pluripotency and even show no differences in the expression level of these genes. When comparing maGSCs with previously published signature genes of other pluripotent cell lines, we found that maGSCs shared a very similar gene expression pattern with embryonic germ cells (EGCs). Also after differentiation of maGSCs and ESCs the transcriptomes of the cell lines are nearly identical which suggests that both cell types differentiate spontaneously in a very similar way. This is the first study, at transcriptome level, to compare ESCs and a pluripotent cell line derived from an adult organism (maGSCs).

 
 
 
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