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Articles by Israr Khan
Total Records ( 2 ) for Israr Khan
  Muhammad Amir Zia , Fazal Hadi , Haji Akbar , Fazal Akbar , Zakir Ullah and Israr Khan
  This research was conducted to characterize B. napus seeds and to compare seeds of different varieties for their compositions. The seeds of ten different Brassica napus varieties namely Cyclon, Star, Vangard, MLCP-048, Bullet, Altex, Con-III, Deffender, Hyola and Dunkled were analyzed for parameters like, pH, electrical conductivity and thousand seed mass. Minerals (Na+ and K+), vitamin C and protein contents were also analyzed. Na+ and K+ were determined by Flame photometer and proteins were analyzed by SDS-PAGE (Sodium dodecyl sulfate polyacrylamide gel electrophoresis). The amount of sodium ranges from 0.020-0.039%. The lowest value of sodium was found in Vangard and the highest in Cyclone. While potassium ranges from 1.14-1.42% with the lowest value for Bullet and the highest for Star. Vitamin C was found in all the varieties in range of 30-96 mg/100 g. The maximum amount of vitamin C was found in Bullet variety while minimum concentration in MLCP-048. Maximum value of pH (6.933) was recorded for MLCP-048, while minimum value for Altex 6.632. The thousand seed mass for cyclone was 5.52 g that was maximum and the lowest value was for bullet variety (3.63 g). Electrical conductivity for different varieties was estimated after different time intervals (20, 40, 60, 80, 100 and 120 min). The overall electrical conductivity determined by conductometer ranged from 0.9-10.4 μ sec. Seeds proteins were analyzed by SDS-PAGE and different numbers of bands showed that variation existed among the different varieties.
  Sikandar , Waqar Ali , Israr Khan and Iqbal Munir
  Tissue culture conditions for two varieties of wheat (Triticum aestivum L.), Fakhr-e-Sarhad and Gandam-2002 were optimized using mature seeds as source of explant. Both of these varieties were tested for their callus production, callus proliferation and regeneration abilities. Different concentrations of 2,4-D (2,4-Dichlorophenoxy acetic acid) were tested for callus induction. Both of these varieties showed maximum callus induction (46.87% for Fakhr-e-Sarhad and 62.5% for gandam-2002) on MS medium supplemented with 3.5 mg L-1 2,4-D. Excellent callus proliferation (87.5% for Fakhr-e-Sarhad and 95.31% for Gandam-2002) was obtained on MS medium containing 4 mg L-1 2,4-D, also callus proliferation (81.25% for Fakhr-e-Sarhad and 93.75% for Gandam-2002) was found on 3 mg L-1 BAP (Benzyl aminopurine). Both varieties were regenerated into whole plant on medium containing 1 mg L-1 IAA (Indol, 3-acetic acid) in combination with 2 mg L-1 BAP after a culture period of 3 weeks. The regeneration was 50 and 60% for Fakhr-e-Sarhad and Gandam-2002, respectively on medium containing 3 mg L-1 Kinetin. The Gandam-2002 produced an average of 4 plantlets per callus, while the Fakhr-e-Sarhad produced an average of 2 plantlets per callus on medium fortified with 1 mg L-1 IAA along with 2 mg L-1 BAP.
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