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Articles by Ismaya
Total Records ( 4 ) for Ismaya
  Rochijan , Budi Prasetyo Widyobroto and Ismaya
  This study was designed to determine the effect of High Rumen Undegraded Protein (HRUP) supplementation on estrous response and profile progesterone hormone in early lactation dairy cows under Indonesia tropical environment. The experiment was conducted with twelve early lactation Friesian Holstein cows divided into two groups. Six cows were used as a control group and other six cows as HURP group. Both control and HRUP group obtained basal diet with forage to concentrate ratio 60:40 (DM basis) but different concentrate composition for each groups. Rumen undegraded protein for control group was 27.47% and HRUP group was 32.78%. Feeding twice daily, morning and afternoon. Water was given by ad libitum. The observed variable were feed intake, estrous response and concentration of progesterone hormone. Data obtained were examined by t-test analysis, except estrous response analyzed descriptively. The result showed that 100% estrous response value was normal and the comparison of progesterone hormone on 1st, 5th, 10th, 15th, 17th, 21st and 26th day between control and HURP group was 0.13±0.02 vs. 0.13±0.04, 0.26±0.24 vs. 0.20±0.08, 0.13±0.03 vs. 1.72±2.23, 0.10±0.04 vs. 1.72±3.77, 0.09±0.02 vs. 3.66±3.76, 0.09±0.01 vs. 0.66±0.80 and 0.12±0.03 vs. 1.20±1.08 ng mL–1. It was concluded that HRUP supplementation on early lactation dairy cows could not increase the concentration of profile progesterone hormone. However estrous response value as well as estrous indication and behavior was normal.
  Rochijan , Budi Prasetyo Widyobroto and Ismaya
  The objective of this study was to determine the impact of High Rumen Undegraded Protein (HRUP) supplementation to blood urea nitrogen and reproduction performance in early lactation dairy cows. Twelve early lactation Friesian Holstein cows were divided into two groups. Six cows were used as a control group and other 6 cows as HRUP group. Both control and HRUP group obtained basal diet with forage to concentrate ratio 60:40 (DM basis), but different concentrate composition for each groups. Rumen undegraded protein for control group was 27.47% and HRUP group was 32.78%. Feeding twice daily, morning and afternoon. Drink water was given by ad libitum. The observed variable were feed intake, performance reproduction (postpartum mating, service per conception, days open) and Blood Urea Nitrogen (BUN). The results indicated that postpartum mating (91.83±31.16 vs. 81.33±19.83 day), service per conception (1.50±0.55 vs. 1.17±0.41 time) and days open (142.33±66.87 vs. 97.33±41.52 day) between controls and HRUP group were not significant (p>0.05). The average BUN value between controls (15.58±1.99 mg dL–1) and HRUP group (18.09±1.50 mg dL–1) were significant (p<0.05). The conclusion is HRUP supplementation in early lactation dairy cows would be impact on BUN value, but no impact on reproduction performance of dairy cows.
  Novia Dimar Dwitarizki , Ismaya , Budi Prasetyo Widyobroto , Sigit Bintara and Tety Hartatik
  This research was aimed to examine the effect of Rumen Undegradable Protein (RUP) supplementation on estradiol profile and estrous behaviour in heifers maintained in tropical climate. A total of 36 Brahman Cross (BX) beef heifers were divided into 2 groups, control group (ration without supplemented RUP) and treatment group (ration with supplemented RUP). The heifers were inseminated on third days after ovaries checking with USG and synchronized with PGF . Blood sampling was conducted to measure the estradiol concentration in 5 points: during the estrous (0); 5; 17; 19 and 21 days. Estrous behaviour were recorded 4 times a day. Results showed no significant effect (p>0.05) of feeding RUP between control and treatment group to estradiol concentration (130.96±40.96; 134.75±39.00; 137.31±47.24; 133.48±36.90; 143.89±55.83 pg mL-1 vs. 126.59±34.86; 124.93±38.82; 123.20±42.39; 126.11±46.45; 126.25±32.66 pg mL-1) and estrous behaviour but there was a significant correlation (p<0.01) between estradiol concentration to estrous behaviour. In conclusion, the level of estradiol concentration and estrous behaviour in heifers were not effected by undegradable protein supplementation.
  Y. Laura , Sri Harimurti and Ismaya
  Objective: The objective of this study was to determine the effect of different dimethylacetamide (DMA) levels on sperm quality of Bangkok rooster chicken and sperm survivability in reproductive tract of hen. Materials and Methods: Sperm was collected from 4 Bangkok rooster chicken aged 1 year. Thirty native hens were used for insemination. Sperm from roosters was collected once a week and cryopreserved 24 h in liquid nitrogen containers at -196°C using 3% (P1), 5% (P2), 10% (P3), 14% (P4) and 18% (P5) DMA levels. Frozen thawed sperm quality was observed after thawing at 4° for 60 sec. The hens were slaughtered and sperm viability was recorded in their reproductive tract on 3, 7, 14 and 21 days after insemination. Results: The results showed that freezing decreased the sperm motility, viability and increased abnormalities. The P2 was observed to be the best DMA concentration for chicken sperm cryopreservation. Different DMA concentrations significantly (p<0.01) affected the motility, viability and abnormalities of frozen thawed sperm. Sperm motility (%) after thawing in P1, P2, P3, P4 and P5 were 38.00±9.08, 46.00±8.94, 16.00±5.48, 8.00±2.24 and 1.00±2.74, respectively. Sperm viability (%) after thawing was 40.10±7.21, 53.50±12.53, 22.00±4.43, 15.30±11.40 and 12.00±3.98 in P1, P2, P3, P4 and P5, respectively. Sperm abnormalities (%) after thawing in P1, P2, P3, P4 and P5 were 60.40±7.40, 41.00±3.32, 67.20±4.09, 68.20±8.58 and 71.00±11.64, respectively. In P2, spermatozoa from the hen’s reproductive tract (vagina, uterus, infundibulum and fimbria) were found motile up to 21 days after insemination. Conclusion: It can be concluded that the best DMA concentration for chicken sperm cryopreservation was 5% level in terms of sperm quality and its survivability in the reproductive tract of hen.
 
 
 
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