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Articles by Ismanizan Ismail
Total Records ( 3 ) for Ismanizan Ismail
  Norzihan Abdullah , Ismanizan Ismail , Vilasini Pillai , Ruslan Abdullah and Shaiful Adzni Sharifudin
  Problem statement: In this study, we identified the full length Coat Protein (CP) gene of the Malaysian Passiflora Virus (MPV) and its 3' non-coding region. The CP gene of the MPV contained 285 amino acid residues. Approach: Pairwise comparison of the MPV CP region with four other potyviruses, namely East Asian Passiflora Virus (EAPV), Passionfruit Woodiness Virus (PWV), Bean Common Mosaic Virus (BCMV) and Soyabean Mosaic Virus (SMV) revealed amino acid sequence similarities ranging from 72-95%. Results: The 3' non-coding region of the MPV, which consists of 255 nucleotides, showed 69-95% nucleotide sequence identity when compared with the four potyviruses. The highest (95%) sequence similarities were detected with PWV and EAPV. An analysis of the deduced amino acid sequences revealed the presence of consensus motifs (DAG tripeptides) characteristic of potyviruses. DAG tripeptides had been reported to be essential for aphid transmission. Conclusion: From the amino acid sequence alignment and identity level observed among the four other potyviruses, we concluded that MPV is a member of the genus Potyvirus and was closely related to both PWV and EAPV.
  Ismanizan Ismail , Norazreen Abdul Rahman , Chan Kok Fei , Zamri Zainal , Nik Marzuki Sidik and Che Radziah Che Mohd Zain
  This research aimed to evaluate the specificity of sesquiterpene synthase promoter (SesqPro) activity in the oil palm tissues and tomato hairy roots and to determine the functional region of the promoter. The effect of jasmonic acid (JA) on the promoter activation and gene expression was also analyzed. A series of 5’ sequence deletions on the full-length SesqPro were generated and individually cloned into the pCAMBIA 1301 vector. Functional analysis was carried out on leaves, mesocarp slices and Immature Embryos (IE) of oil palm and tomato hairy roots that had been transformed with full-length SesqPro (PSPr-VF6). GUS expression was found in all the tissues and a higher activity was detected in IE and mesocarp slices. All the constructed derivatives of SesqPro were transformed into IE and mesocarp slices in order to determine the promoter regions which are responsible for gene expression. The reduction of GUS activity was found to be related to the removal of DNA sequences within the promoter region. The promoter was induced by the elicitor molecule JA, thus suggesting the presence of JA responsive elements within the promoter. Incubation with 100 μM of JA showed higher GUS activity in IE and mesocarp slices that had been transformed with PSPr-VF4 to PSPr-VF6. Nevertheless, the GUS activity was drastically reduced in IE and mesocarp slices containing the PSPr-VF3 promoter, suggesting that the presence of the G/A hybrid box located at -622 to -617 act as a specific element in response to elicitors. This study has shown that the action of SesqPro is non-specific and was influenced by JA induction.
  Alina Wagiran , Ismanizan Ismail , Che Radziah Che Mohd Zain and Ruslan Abdullah
  Improvement on plant regeneration system from suspension cells culture of different japonica rice cultivar was attempted in the present study. The present study shows that callus induction varied depend on genotype. Nipponbare, Hayahishiki and Fujisaka 5 variety showed 100, 93 and 53% of callus induction respectively on LS medium supplemented with 2,4-D after 4 weeks in culture. Callus proliferation was observed at the scutellum and yellowish in colour. Suspension cell culture was initiated from 4 weeks old callus in N6 liquid medium contained with 3 mg L-1 2,4-D and 1 mg L-1 kinetin for one month with regular subculture. The embryogenic cells were characterized with 1% (w/v) Evans Blue. Primary study shows that shoot regeneration was highest on Nipponbare at 60%, while Hayahishiki and Fujisaka were at 30 and 20%, respectively when culture on MS basal medium contained 3 mg L-1 Kinetin and 0.5 mg L-1 NAA (SRM3). After 6 weeks, the callus covered with green shoot bud and later formed shoot with an average of 3-8 shoot per callus. Increase of shoot regeneration was observed when callus treated with partial desiccation in all variety tested. The regeneration frequency was highest at 76, 70 and 33% when Nipponbare, Hayahishiki and Fujisaka 5, respectively were treated with 48 h desiccation. However, shoot multiplication was low in 0 h desiccation ranging from 10-16%. After 8 weeks in optimal SRM medium, the plantlets was hardened in growth chamber for 2 weeks and later transferred into soil with 100% survival in all variety. The present study shows that rice regeneration system from suspension cells can be improved with modification of plant growth regulators and desiccation.
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