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Articles by Islam Nour
Total Records ( 2 ) for Islam Nour
  Hala El- Adawi , Islam Nour , Faiza Fattouh and Nehal El- Deeb
  This study was designated to investigate the antiviral activity of the extracellular extract of Lactobacillus bulgaricus 761N against Hepatitis C virus. The cytotoxicity of the extracellular extract of L. bulgaricus 761N assay was conducted on two cell lines involving the Human Liver Hepatocellular Carcinoma (HepG2) cell line and the human Peripheral Blood Mononuclear Cells (PBMC). The antiviral capacity of the extracellular extract of the candidate strain against Hepatitis C Virus (HCV) was tested using real time quantitative polymerase chain reaction (RTqPCR). The pre-treatment trial was found to be of a higher a ntiviral activity than the post-treatment trial. It was found that it possesses also a moderate antioxidant activity that was observed to be higher on HepG2 cell line than on PBMC. Purification by the fractionation process using size exclusion chromatography revealed the presence of two fractions (8 and 15) that were confirmed by SDS-PAGE and were of molecular weights 14.4 and 45 kDa, respectively. Fraction 8 was found to be of a higher antiviral bioactivity in case of pretreatment trial than fraction 15. In case of post-treatment trial, fraction 8 was also observed to be of a higher antiviral capacity than fraction 15.
  Islam Nour , Faiza Fattouh and Hala El-Adawi
  Bacteriocin producing lactobacilli were obtained from different sources and were previously characterized outdoors. Lactobacillus bulgaricus 761N, Lactobacillus fermentum DSMZ 20049, Lactobacillus delbrueckii subsp. bulgaricus NCTC 12197 T and Lactobacillus delbrueckii subsp. bulgaricus DSMZ 20080T were the strains under study. Subcellular fractions were investigated for their proteolytic activity that results in production of bioactive peptides in the cell-free supernatant (bacteriocin). A single strain bacteriocin was selected depending upon cytotoxicity of the strain’s bacteriocin and antibacterial capacity. The cell-free supernatants of the tested strains were tested for their antibacterial capacity against a broad spectrum of pathogens involving Staphylococcus aureus, Pseudomonas aeruginosa, Burkholderia cepacia, Salmonella spp., Escherichia coli and Shigella spp. The selected candidate was Lactobacillus bulgaricus 761N. The bacteriocin was partially purified and assured via applying the semi-purified samples on SDS-PAGE. Advance to the purification process, the fractions selected were tested for their antibacterial capacity as well. Both fractions have been found to possess antibacterial activity but the lower molecular weight was over succeeded.
 
 
 
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