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Articles by Immaculate Jeyasanta
Total Records ( 3 ) for Immaculate Jeyasanta
  Immaculate Jeyasanta , K. Hermina Giftson and Jamila Patterson
  Background and Objective: Study evaluates the suitable chemical method for the determination of the freshness of refrigerated seafood. Hypoxanthine content (Hx) of the refrigerated seafood was analysed and it was compared with the level of volatile amines production due to bacterial spoilage. Materials and Methods: Fresh seafood such as Stolephorus commersonii, Scomberomorus koreanus and Portunus sanguinolentus were collected from the fishing harbour of Tuticorin. The fishes were analyzed initially for TMA-N, TVB-N, hypoxanthine and pH content and it was stored at -4°C for 30 days. Sampling was done for every 10 days interval and it was checked for all the above quality indicators. Results: The results showed that volatile amines (TVB-N) and (TMA-N) were not found in stored seafood till the end of 10 days storage. Changes in pH and hypoxanthine content were noticed at the initial storage period itself. Refrigeration of sea foods are common at homes and at this condition even the superior quality stored fish has hypoxanthine formation and it gradually increase during the storage period. Hx assay reflects ATP break down and ATP-related compounds and the degradation of ATP to Hx has been attributed to muscle endogenous autolytic enzymes. The enzymatic reaction occurs in the initial stage of storage at low temperature but the contents of trimethylamine-nitrogen and total volatile base-nitrogen increase progressively during the later period of spoilage. Thus, hypoxanthine is regarded as the major catabolite of adenosine triphosphate while it was formed in refrigerated situation and it is a valuable freshness marker in stored fish. Conclusion: Thus, the study reveals that Hx measurements have some advantage over TMA-N and TVB-N analyses and Hx could be used as a superior spoilage indicator in the refrigerated sea foods. This study proves that the Hx could be used as a spoilage indicator and the sea foods stored at -4°C, should be consumed within 10 days of storage period to avoid Hx accumulation.
  Immaculate Jeyasanta , Narmatha Sathish and Jamila Patterson
  Background and Objective: Anti-bacterial activity was evaluated in the whole body crude extracts of the 3 edible marine bivalves, namely Perna perna (brown mussel), Perna viridis (green mussel) and the parrot mussel collected from the coastal villages of Kanyakumari district. Materials and Methods: Six different solvents, namely ethanol, methanol, acetone, ethyl acetate, hexane and butanol were used to prepare the extracts. The efficacies of the whole body extract of different solvents were assessed for the antimicrobial activity against 13 different pathogenic bacteria viz., Salmonella typhi, Escherichia coli, Pseudomonas aeruginosa, Staphylococcus aureus, Proteus vulgaris, Staphylococcus epidermis, Klebsiella pneumoniae, Bacillus subtilis, Salmonella paratyphi, Streptococcus pyogenes, Vibrio sp., Shigella sp. and Haemophilus influenza. Results: Of the 6 different solvents methanol exhibits the highest activity followed by ethanol and acetone. The methanolic extracts of P. viridis exhibit the highest activity against E. coli, S. typhi and P. aeruginosa. The ethanolic extract of P. viridis shows the highest activity against E. coli, P. aeruginosa, S. typhi, K. pneumoniae and acetone extract showed good activity against E. coli. The methanolic extracts of P. perna exhibit the highest activity against E. coli, S. aureus, S. typhi, P. vulgaris. The ethanolic extract of P. perna is active against P. vulgaris and B. subtilis and the ethanolic extract showed good activity against P. aeruginosa and Shigella species. The methanolic extracts of the parrot mussel exhibit the highest activity against E. coli and Streptococcus pyogenes, ethanol extract shows good activity against E. coli and Staphylococcus aureus and the acetone extract shows maximum antibacterial activity against P. aeruginosa. Conclusion: The findings of the present study confirm that the mussel species have antimicrobial activity and that this activity appears to be dependent on the solvent used for the extraction process. FTIR analysis reveals the presence of bioactive compounds signals at different ranges.
  Immaculate Jeyasanta and Jamila Patterson
  Background and Objective: Indian squid Loligo duvauceli is common major sources of seafood available in Tuticorin. However, the ink of these cephalopods is considered as the waste product. In the present study, the L. duvauceli ink was taken as a raw material which is generated as a byproduct in the processing industry, has low market value and has the potential to cause environmental pollution. Materials and Methods: The ink samples were screened for phytochemical, functional properties, proximate, antibacterial and antioxidant activities by standard methods. Results: Large numbers of compounds are present in the L. duvauceli ink are amino acids, carbohydrates, phenols, flavonoids, alkaloids and saponins whereas, tannins, steroid, glycosides are present in a small amount and anthraquinones, fixed oils and fats are absent. It had moisture, protein, lipid, ash and carbohydrate content were 79.25±0.051, 10.67±0.005, 0.19±0.01, 0.15±1.78 and 0.91±0.29%, respectively and this study showed that squid ink had the highest proximate composition and nutritionally preferred. The functional property of ink had pH (6.52±0.03) and water solubility index (16.8±0.004%) and the study revealed that ink can be used as a natural emulsifier in food applications. This investigation showed the methanol extract of ink exhibited significant activity against pathogens Escherichia coli, Klebsiella pneumoniae and Pseudomonas aeroginosa. The partially purified form of L. duvauceli ink also tested for antioxidant activity such as enzymatic antioxidants, nitric oxide scavenging activity and total antioxidant activity. Conclusion: This study suggests that L. duvauceli ink extract exhibit great potential for antioxidant activity and may be useful for their nutritional and medicinal functions. These results concluded that the L. duvauceli ink is a novel potential product and it has to further characterize to improve the pharmacological active marine natural products.
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