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Articles by Iman Rusmana
Total Records ( 4 ) for Iman Rusmana
  Nisa Rachmania Mubarik , Irni Mahagiani , Amaryllis Anindyaputri , Sugeng Santoso and Iman Rusmana
  Problem statement: Chitin, a common constituent of insect exoskeleton, could be hydrolyzed by chitinase. The research was conducted to screen chitinolytic rhizobacteria isolated from rhizosphere of chilli pepper and to determine their chitinase activity in degrading chitin of whitefly, Bemisia tabaci Genn. (Hemiptera: Aleyrodidae). Whitefly is recognized as an important pest on many crops including chilli pepper. Approach: Screening and molecular identification based on 16S rRNA sequence of chitinolytic isolates, chitinase productions, measurement of chitinase activity, characterization of chitinase and effect of the chitinase treatment on whitefly were studied. Results: A total of 25 isolates of rhizobacteria formed a clear zone on solid chitin media. Two isolates, i.e., I.5 and I.21 isolates had the highest chitinolytic index. Based on sequence of 16S rRNA gene, the isolates of I.5 and I.21 were identified as Bacillus sp. and Bacillus cereus, respectively. The highest chitinolytic index and specific activity of I.5 isolate was 0.94 and 0.11 U mg-1 proteins, respectively. Maximum production of I.5 chitinase was occured after 36 h cultivation at 30°C and pH 7.0. The highest chitinolytic index and specific activity of I.21isolate was 0.75 and 0.114 U mg-1 proteins, respectively. Maximum production of I.21 chitinase was occured after 36 h cultivation at 55°C and pH 7.0. Cell culture and crude enzyme of the isolates were tested on chitin of B. tabaci and the effect was observed using a microscope and sterile water was used as a negative control. Hydrolytic observation showed that crude enzyme of I.21 isolate could degrade chitin of B. tabaci exoskeleton and the activity was better than that of I.5 isolate. Conclusion: Chitinase produced by Bacillus cereus I.21 strain has potential application as biocontrol agents for B. tabaci.
  Hessy Novita , Iman Rusmana , Munti Yuhana and Fachriyan Hasmi Pasaribu
  Motile Aeromonad Septicaemia (MAS) disease in catfish (Clarias gariepinus) is caused by infection with Aeromonas hydrophila. One alternative way to control infection by this bacteria is applying probiotics, which inhibit quorum sensing, also known as Anti-Quorum Sensing (AQS), using Bacillus sp. administered by feeding. Bacillus sp. product AHL (Acyl Homoserine Lactone) Lactonase, which is speculated to be able to inhibit quorum sensing in pathogenic bacteria. In vivo test of probiotics was set up in triplicate and consisted of five treatments. Effect of AQS probiotic bacteria as feed supplement was set up in improving survival rate, Feed Conversion Ratio (FCR), Specific Growth Rate (SGR) and non specific immune response of catfish (Clarias gariepinus). The result showed that the fish feed with AQS had a high percentage of survival rate. The AQS mix of treatment (TS1RifR, TS2RifR and TA23RifR) had the highest survival rate (93%). The AQS treatments were also had values of FCR and SGR better than that of control. However, the control treatments had survival rate only 31%. The AQS isolates could also improve a non specific immune response. Result indicated that AHLs degrading bacteria could be considered as better alternative to replace application of antibiotics in aquaculture as biocontrol of bacterial fish disease and in reducing the pathogenicity of A. hydrophila in catfish (Clarias gariepinus).
  Susan Soka , Antonius Suwanto , Dondin Sajuthi and Iman Rusmana
  Tempeh is a popular Indonesian fermented food made from soybean that can be a good source of dietary fibers for human health. Dietary fibers have the ability to modulate gut microbiota composition in order to improve human health. In this study, Sprague-Dawley (SD) rats were fed a standard diet supplemented with cooked and de-hulled soybean or tempeh for 28 days. The specific bacterial groups in fecal samples were quantified using real-time polymerase chain reaction with 16S rRNA gene-targeted, group-specific primers. Populations of Bacteroidetes, Bacteroides fragilis, Firmicutes and Clostridium leptum increased when supplemented with tempeh. In addition, the ratio of Firmicutes/Bacteroidetes was lower in groups fed with either raw or cooked tempeh compared to the soybean group. Previous studies showed that obese hosts have higher ratios of Firmicutes/Bacteroidetes compared to the lean hosts. Increased Bacteroidetes populations after tempeh supplementation indicated that tempeh might modulate the composition of gut microbiota toward a healthier gut.
  Yuni Puji Hastuti , Iman Rusmana , Kukuh Nirmala and Ridwan Affandi
  Backgorund: The activities of NH4+ and NO2 oxidizing bacteria in aquaculture recirculating system can influence to environmental N-inorganic, physiologic response and growth performance of mud crab Scylla serrata. The culture of mud crab in a Recirculating Aquaculture System (RAS) involves the microbiological nitrogen removal including aerobic and anaerobic nitrogen conversion such as nitrification and denitrification. In nitrification process activities of ammonium (NH4+) and nitrite (NO2) oxidizing bacteria can influence for a Dissolved Inorganic Nitrogen (DIN) like as ammonia (NH3), nitrite (NO2) and nitrate (NO3) in the RAS environmental. Objective: The objective of this study aimed to isolate and determine the activities of ammonium (NH4+) and nitrite (NO2) oxidizing bacteria in crab culture using a recirculating aquaculture system. Materials and Methods: With different quantities of shelters (4 and 6 shelters) in box cultivation of mud crab, the activity oxidizing bacteria be count by amount of NH4+, NO2 and NO3. The concentrations of ammonia, nitrite and nitrate be measured with spectrophotometers in the Environmental and Microbiology Laboratory, Bogor Agricultural University. Ammonium (NH4+) and nitrite (NO2) oxidizing activity tests were used to determine the enzyme activity and affinity to the culture substrate. Results: Based on the Michaelis-Menten kinetics analysis for ammonia oxidizer, it was found that treatment P4 showed a Vmax value (Maximum speed) of 0.073 mM h–1 and a Km (Michaelis-Menten) constant was 0.1909 mM. Whereas treatment P6 showed lower values, i.e., Vmax of 0.0673 mM h–1 and Km of 0.0788 mM, indicating that the affinity of enzymes related to ammonium oxidation by the bacteria in this treatment was higher. Conclusion: This study showed P6 give the best of NH4+ oxidizer activity and this was supported by the higher ammonium oxidation rate (12.72±5.89 μM h–1).
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