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Articles by Imam Megantara
Total Records ( 2 ) for Imam Megantara
  Keri Lestari , Haig Babikian , Yusef Babikyan , Imam Megantara , Rajeev Kumar Jha , Arif S.W. Kusuma , Margaretha Prayudhi Novantiana and Fransiskus Xaverius Sudirman
  Background and Objective: Avian Infectious Bronchitis Virus (AIBV) is a highly infectious pathogen of chicken. There is no medication and vaccine developed yet due to its highly mutable nature. The objective was to formulate a blend that can be effective against the viral infection in chicken. Material and Methods: Several natural essential oils, like, Gardenia jasminoides, Commiphora myrrha, Boswellia serrata, Foeniculum vulgare and Daucus carota with anti-viral properties were blended and tested against Avian Infectious Bronchitis Virus H120 (AIBV H120) strain using in vitro medium. The trial was conducted in an allantoic fluid medium. Different concentration of NOB was inoculated into the allantoic-fluid to challenge with the lethal dose of virus. Results: The Natural Oil Blend (NOB), at its minimum concentration up to 0.01 mL of 0.1%, was effective against the AIBV H120 strain. The NBO concentration lower than 0.1% was not sufficient to deactivate the AIBV H120 strain. The embryos up to 0.1% of NBO treated groups and negative control embryos were alive and tested negative to AIBV H120 strain, whereas the embryos in positive control died and tested positive. Conclusion: The in vitro trial proven that the essential oil Blend can deactivate the Avian Infectious Bronchitis Virus H120 (AIBV H120) in in vitro medium.
  Imam Megantara , Ronny Lesmana , Nova Sylviana , Adi I. Cahyadi and Sunarjati Sudigdoadi
  Background and Objective: Detection of methicillin-resistant S. aureus have become a challenge in the presence of oxacillin-susceptible and mecA-positive S. aureus (OS-MRSA), concerning the misidentification events and therapeutic implications. This study aims to identify the OS-MRSA in clinical isolates of Post-viral acute rhinosinusitis, which, hopefully, can interfere with the therapeutic strategy. Materials and Methods: There were 60 patients diagnosed with Post-viral acute rhinosinusitis, recruited from an Ear, Nose and Throat (ENT) outpatient clinic. Staphylococcus aureus isolates were identified from the culture and were then tested for antibiotics susceptibility using a Kirby-Bauer disc diffusion test. The mecA, mecC and blaZ genes were determined using the Polymerase Chain Reaction (PCR) method. Results: Staphylococcus aureus was identified in 20 of the 60 samples from the patients (33.3%; 95% CI: 21.0-45.6). Of the 20 isolates, 19 isolates (95%) had a positive mecA gene, 19 (95%) had a positive mecC gene and 20 (100.0%) had a positive blaZ gene. The majority of the mecA-positive S. aureus showed an oxacillin-susceptible (85%) and 3 isolates (15.0%) were oxacillin-resistant toward the S. aureus. Conclusion: There was a high proportion of Oxacillin/cefoxitin-Susceptible mecA-positive S. aureus in the study population that indicate phenotypic susceptibility to antibiotics does not always indicate the absence of genes that carry resistant traits, thus allowing misidentification if the only phenotypic examination is carried out.
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