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Articles by Ilker DURAK
Total Records ( 3 ) for Ilker DURAK
  Aslihan AVCI , Recep CETIN , Imge B. ERGUDER1 , Erdinc DEVRIM , Bulent KILICOGLU , Ozden CANDIR , H. Serdar OZTURK1 and Ilker DURAK

Aims: In this study, we aimed to investigate the possible molecular mechanism of cisplatin hepatotoxicity and to establish whether some natural antioxidant foods, namely dried black grape and tomato, may provide protection against cisplatin hepatotoxicity.

Materials and Methods: Twenty-eight rats were used throughout the study. Cisplatin was administered intraperitoneally (i.p.) in a single dose (10 mg/kg). Antioxidant food supplementation was started three days before cisplatin treatment. There were 7 animals in each group (control, cisplatin, cisplatin plus dried black grape and cisplatin plus tomato juice). Rats were sacrificed 72 h after the treatment. The livers were removed and prepared for the biochemical and histopathological investigations. Oxidant and antioxidant parameters were measured in liver tissues of the groups.

Results: Malondialdehyde (MDA) level and xanthine oxidase (XO) activities were higher in the cisplatin group compared with the control values. Catalase (CAT) and glutathione peroxidase (GSH-Px) activities were higher but MDA level lower in the grape-supplemented group compared with the cisplatin group. In the histopathological examination, sinusoidal congestion, hydropic and vacuolar degeneration, extensive disorganization in hepatocytes, and significant fibrosis around central venules and expanded periportal areas were observed in the liver tissues from cisplatin-treated animals. In rats treated with cisplatin and fed with tomato juice, sinusoidal congestion was less in comparison to the cisplatin-treated group and no hepatocyte disorganization or hydropic degeneration was seen. In rats treated with cisplatin and dried black grape, disorganization of hepatocytes was mild in comparison to cisplatin-treated animals. Perivenular and periportal fibrosis was mild.

Conclusions: Results suggest that cisplatin treatment causes significant oxidant load to the liver through both XO activation and impaired antioxidant defense system, which result in accelerated oxidation reactions in the liver tissue. Additionally, cisplatin treatment resulted in significant harmful effects on hepatocytes. We propose that supplementation with some antioxidant foods with high antioxidant power may ameliorate this toxicity.

  Aslıhan AVCI , Teslime ATLI , B. Imge ERGUDER , Murat VARLI , Erdinc DEVRIM , Ozgur DEMIR , Ilker DURAK and Murat TURGAY
  To investigate the effects of ingesting Fructus vitis minuta (black grape) on plasma and erythrocyte antioxidant parameters in elderly subjects. Materials and methods: Thirteen subjects (mean age 74.67 0.58) participated in the study. They consumed 1 g/kg body weight per day of dried black Maras, grapes for 1 month. Before and after these periods, fasting blood samples were obtained, and oxidant (as MDA) and antioxidant (as SOD) parameters were studied in erythrocytes. Also, plasma samples were obtained from the subjects. Results: In the erythrocyte hemolysate, MDA (malondialdehydes) levels and SOD (superoxide dismutase) activities were found to be lower (P < 0.01) in the second samples compared to the first ones. Conclusion: We think that because free radical production was decreased SOD activities were not increased. Our results show that reduced peroxidation processes caused by Maras, grape consumption suggest that grape consumption has beneficial effects in elderly subjects.
  Aslıhan AVCI , Asuman SUNGUROGLU , İmge B. ERGUDER , Guvem GUMUS AKAY , Erdinc DEVRIM , Pınar OZKAL BAYDIN , Nuray VAROL and Ilker DURAK
  Aims: To investigate the possible effects of aqueous garlic extract on the oxidant/antioxidant status and apoptosis in 32D (wild type mouse myeloid cell = normal) and 32Dp210 (BCR-ABL fusion gene (+) mouse myeloid cell = Chronic Myelocytic Leukemia cells) cell lines. Materials and methods: Aqueous garlic extract (10% w/v) was added into the cell line media with 2 different final concentrations (0.4% and 1%). At 0 h and at 24, 48, and 72 h later, the oxidant (malondialdehyde (MDA) level, and xanthine oxidase (XO) enzyme activity) and antioxidant (superoxide dismutase (SOD), glutathione peroxidase (GSH-Px), and catalase (CAT) enzyme activities) parameters were measured in the cell lines. Results: It was observed that the garlic extract caused no change in the XO and antioxidant enzyme activities, but it increased the MDA level in the 32D cell line. However, significant increases were found in the MDA level, XO, and antioxidant enzyme activities in the 32Dp210 cell line treated by the garlic extract. Additionally, it was shown that garlic extract had antiproliferative and apoptotic effects on both cell lines. The most effective apoptotic dose was found to be 0.4% (w/v), and at this concentration the death risk of the 32Dp210 cell line was calculated at 2.08 times higher than that of the 32D cell line. Conclusion: It has been suggested that garlic directly causes oxidant stress in the 32D cell line owing to its own oxidant ingredients, and that the oxidant stress created by garlic in the 32Dp210 cell line might occur through increased XO activity and/or its oxidant ingredients. Additionally, antioxidant enzyme activities were found to increase in the 32Dp210 cell line; it would seem that this compensatory change could not prevent the oxidant stress created. We think that the oxidant potential of garlic extract might play a part in its possible anticancer potential, previously supposed by several investigators.
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